Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   Q9L40_RS14005 Genome accession   NZ_CP132190
Coordinates   1904052..1904480 (-) Length   142 a.a.
NCBI ID   WP_305798924.1    Uniprot ID   -
Organism   Vibrio cholerae strain DRC187     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1899052..1909480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9L40_RS13985 (Q9L40_13985) coaE 1899550..1900158 (-) 609 WP_162891880.1 dephospho-CoA kinase -
  Q9L40_RS13990 (Q9L40_13990) pilD 1900155..1901030 (-) 876 WP_000418747.1 A24 family peptidase Machinery gene
  Q9L40_RS13995 (Q9L40_13995) pilC 1901088..1902314 (-) 1227 WP_305798925.1 type II secretion system F family protein Machinery gene
  Q9L40_RS14000 (Q9L40_14000) pilB 1902357..1904045 (-) 1689 WP_044127953.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q9L40_RS14005 (Q9L40_14005) pilA 1904052..1904480 (-) 429 WP_305798924.1 pilin Machinery gene
  Q9L40_RS14010 (Q9L40_14010) nadC 1904725..1905615 (-) 891 WP_305798923.1 carboxylating nicotinate-nucleotide diphosphorylase -
  Q9L40_RS14015 (Q9L40_14015) ampD 1905913..1906458 (+) 546 WP_088128396.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  Q9L40_RS14020 (Q9L40_14020) - 1906577..1907965 (-) 1389 WP_305798922.1 IS4-like element ISVa18 family transposase -
  Q9L40_RS14025 (Q9L40_14025) fldB 1908249..1908767 (-) 519 WP_000690112.1 flavodoxin FldB -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15127.33 Da        Isoelectric Point: 7.7758

>NTDB_id=795627 Q9L40_RS14005 WP_305798924.1 1904052..1904480(-) (pilA) [Vibrio cholerae strain DRC187]
MNAYKNKQQQGFTLIELMIVVAIIGVLAAIAVPAYKDYVTKSEASSAIATLKALQTPAELLYQENGNLTVSNALAALGTS
ENANPLGKLEVEGTDGLKFTFTQSGALKDQTITLQRSGTDGWKCNASDKIEKLKIKSCTTAQ

Nucleotide


Download         Length: 429 bp        

>NTDB_id=795627 Q9L40_RS14005 WP_305798924.1 1904052..1904480(-) (pilA) [Vibrio cholerae strain DRC187]
ATGAACGCGTATAAAAACAAACAACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTAGCGATTATTGGTGTGTT
AGCAGCGATTGCTGTGCCTGCTTATAAGGATTATGTCACTAAGAGTGAAGCATCCTCAGCTATCGCAACACTGAAAGCAC
TGCAGACCCCTGCGGAGCTACTCTATCAAGAGAATGGTAATTTAACCGTTAGTAATGCCCTAGCAGCATTAGGTACATCA
GAAAACGCCAACCCGCTGGGAAAATTGGAGGTAGAGGGTACTGATGGGTTAAAGTTTACCTTCACACAATCAGGAGCCTT
GAAAGATCAAACCATAACCTTACAGAGAAGTGGTACTGACGGTTGGAAATGTAATGCTTCTGACAAAATTGAAAAACTCA
AGATAAAATCTTGTACAACAGCACAATAG

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

52.756

89.437

0.472
  pilA Pseudomonas aeruginosa PAK

42.282

100

0.444
  pilA Vibrio cholerae strain A1552

40.26

100

0.437
  pilA Vibrio cholerae C6706

40.26

100

0.437
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.26

100

0.437
  pilA Acinetobacter baumannii strain A118

38.356

100

0.394
  pilA2 Legionella pneumophila strain ERS1305867

38.129

97.887

0.373
  pilA2 Legionella pneumophila str. Paris

37.41

97.887

0.366