Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PWO56_RS01985 Genome accession   NZ_CP118699
Coordinates   410594..412300 (-) Length   568 a.a.
NCBI ID   WP_029302067.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain K522     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 405594..417300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWO56_RS01955 (PWO56_01955) mutT 406271..406684 (+) 414 WP_179101298.1 8-oxo-dGTP diphosphatase MutT -
  PWO56_RS01960 (PWO56_01960) yacG 406745..406939 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  PWO56_RS01965 (PWO56_01965) zapD 406949..407671 (-) 723 WP_024945431.1 cell division protein ZapD -
  PWO56_RS01970 (PWO56_01970) coaE 407709..408323 (-) 615 WP_077095241.1 dephospho-CoA kinase -
  PWO56_RS01975 (PWO56_01975) pilD 408342..409214 (-) 873 WP_077095240.1 A24 family peptidase Machinery gene
  PWO56_RS01980 (PWO56_01980) pilC 409237..410478 (-) 1242 WP_043126919.1 type II secretion system F family protein Machinery gene
  PWO56_RS01985 (PWO56_01985) pilB 410594..412300 (-) 1707 WP_029302067.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  PWO56_RS01990 (PWO56_01990) - 412304..412744 (-) 441 WP_077095239.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PWO56_RS01995 (PWO56_01995) nadC 413067..413924 (-) 858 WP_077095238.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PWO56_RS02000 (PWO56_02000) - 413930..414400 (-) 471 WP_024944772.1 retropepsin-like aspartic protease -
  PWO56_RS02005 (PWO56_02005) ampD 414548..415120 (+) 573 WP_077095237.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PWO56_RS02010 (PWO56_02010) pdhR 415396..416160 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62511.90 Da        Isoelectric Point: 5.4342

>NTDB_id=794444 PWO56_RS01985 WP_029302067.1 410594..412300(-) (pilB) [Aeromonas hydrophila strain K522]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=794444 PWO56_RS01985 WP_029302067.1 410594..412300(-) (pilB) [Aeromonas hydrophila strain K522]
ATGACTTCTAGTCCCAATAGCGGCTTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
TCTGAGCCAAGCCAAGGCACAGCGCAAACCTTTTGTGACCTTTCTGATCGAAAACGAAATCCTCGACAGCAAAGCATTGG
CGGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAAAAACATCATGTCCTGCCCATTTACACCCAGGGGCACACCCTCTATATCGCCATGTCGGA
TCCCACCAATGTCTCTGCACTGGAGGATTTTGGTTTCAGCTTCGGGTTGCATACCGAAGCGCTGCTGGTAGAGGAGAACA
AACTCACCACAGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAACTGGAAGTGTCTGACGAGGGATCGCGGCTCGACGAGAGCGTCAATACGGCCGACGACGACGCCCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGAGCCTCCGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAAATAGCGACTCCACCAGTCAATCTGGCCAACCGCTTCTCGGCTCGC
CTCAAGGTGATGGCACGGCTCGATATCGCCGAGCGGCGACTGCCCCAGGATGGTCGCATCAAACTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCGACTCCTCCG
CGGCACGTCTCAATATCGAGCAGCTCGGTTTCGACGATCGGCAGAAGGCGCAATATCTGCGTGCTCTGTCGAAGCCCCAG
GGCATGATCCTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTTTCCCTCTATACCGGTCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAGGTCAATCCCAAGG
CAGGGCTGACCTTCGCGAGCGCCCTGCGCTCTTTCTTGCGCCAAGACCCCGATGTGGTCATGGTGGGTGAAATCCGGGAT
CTGGAAACAGCCGAAATTGCCATCAAGGCCGCCCAGACAGGTCACCTGGTGCTATCCACCCTGCACACCAACTCGGCGGC
GGAAACGCTGACCCGGATGATGAACATGGGGGTCCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTGGCGCGTAAATTGTGCGATCACTGCAAAGCCCCCGAAGTGGTACCCGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCTGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCGGGTGGCTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAAGGGGCCAACTCTCTGCAGATTGCCG
CCATCGCCCAGAAGGAAGGCATGCGTACCTTGCGTACCTCCGGCCTTGAGAAGGCCCGCCTGGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Legionella pneumophila strain ERS1305867

54.336

99.472

0.54
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

51.943

99.648

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373