NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	PWF74_RS10175	Genome accession	NZ_CP118627
Coordinates	2037233..2038177 (+)	Length	314 a.a.
NCBI ID	WP_165705597.1	Uniprot ID	A0AAX3NG78
Organism	Lactococcus garvieae strain Pa-2
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Prophage	2019644..2036746	2037233..2038177	flank	487

Gene organization within MGE regions

Location: 2019644..2038177

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PWF74_RS10095 (PWF74_10095)	-	2019653..2020519 (+)	867	WP_165705498.1	glycosyltransferase	-
PWF74_RS10100 (PWF74_10100)	-	2020522..2021253 (+)	732	WP_003133698.1	glycosyltransferase family 2 protein	-
PWF74_RS10105 (PWF74_10105)	-	2021253..2021618 (+)	366	WP_017370495.1	DUF2304 domain-containing protein	-
PWF74_RS10110 (PWF74_10110)	-	2021602..2022546 (+)	945	WP_274978357.1	NAD-dependent epimerase/dehydratase family protein	-
PWF74_RS10115 (PWF74_10115)	-	2022998..2024110 (+)	1113	WP_165705500.1	polysaccharide pyruvyl transferase family protein	-
PWF74_RS10120 (PWF74_10120)	-	2024148..2025677 (+)	1530	WP_165705501.1	lipopolysaccharide biosynthesis protein	-
PWF74_RS10125 (PWF74_10125)	-	2026033..2027172 (+)	1140	WP_165705502.1	glycosyltransferase family 4 protein	-
PWF74_RS10130 (PWF74_10130)	-	2027169..2028002 (+)	834	WP_165705503.1	LicD family protein	-
PWF74_RS10135 (PWF74_10135)	-	2028016..2029008 (+)	993	WP_165705504.1	glycosyltransferase family 2 protein	-
PWF74_RS10140 (PWF74_10140)	-	2029010..2029897 (+)	888	WP_165705505.1	glycosyltransferase family 2 protein	-
PWF74_RS10145 (PWF74_10145)	tagD	2029922..2030338 (+)	417	WP_165705506.1	glycerol-3-phosphate cytidylyltransferase	-
PWF74_RS10150 (PWF74_10150)	-	2030363..2032087 (+)	1725	WP_165705507.1	surface protein	-
PWF74_RS10155 (PWF74_10155)	-	2032097..2033464 (+)	1368	WP_165705508.1	DUF2142 domain-containing protein	-
PWF74_RS10160 (PWF74_10160)	-	2033495..2035096 (-)	1602	WP_165705509.1	GH25 family lysozyme	-
PWF74_RS10165 (PWF74_10165)	guaB	2035265..2036746 (+)	1482	WP_165705510.1	IMP dehydrogenase	-
PWF74_RS10170 (PWF74_10170)	rpsU	2036869..2037045 (+)	177	WP_003134539.1	30S ribosomal protein S21	-
PWF74_RS10175 (PWF74_10175)	comGA	2037233..2038177 (+)	945	WP_165705597.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

Download Length: 314 a.a. Molecular weight: 35862.11 Da Isoelectric Point: 6.7731

                                >NTDB_id=794068 PWF74_RS10175 WP_165705597.1 2037233..2038177(+) (comGA) [Lactococcus garvieae strain Pa-2]

MIQVLAKTLLQKAVDQMVHDLYFVALEGRYSLYFRTATERRFERDLTLEQGQALIAHMKFLSGMNLGESRRVQLGACTYV

LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFKSFIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI

CIEDPVELVETEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR

LKELGLTDWELQSSLQRVIYQRLIAGKGLLVYEKEKFEEWQPDEWNGQIDQLVADGFISAVTAAYEKIEFSEAD

Nucleotide

Download Length: 945 bp

                                >NTDB_id=794068 PWF74_RS10175 WP_165705597.1 2037233..2038177(+) (comGA) [Lactococcus garvieae strain Pa-2]

ATGATACAGGTTTTGGCAAAAACCCTTTTACAAAAAGCAGTAGATCAGATGGTGCATGATCTTTATTTCGTAGCATTAGA

AGGAAGGTATTCTCTCTATTTTCGCACAGCAACAGAAAGGAGGTTTGAAAGAGATCTCACCCTTGAACAAGGGCAAGCAC

TGATTGCTCATATGAAATTTTTATCTGGTATGAATTTAGGAGAAAGTAGGCGGGTACAGTTGGGGGCTTGCACTTATGTT

TTAGATAAAGGTGAGCAGCGTTTACGTTTATCTACAGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATCCGACTTCT

GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATTTTTAAATCTTTTATTGGTGGGAGAGGGCTTTACCTGT

TTTCCGGTCCCGTAGGCTCAGGTAAGACCAGCCTTATGTACAAGTTTGCAAGGGAACATTTTAAAAATCAACAAGTTATT

TGTATAGAAGATCCTGTGGAGTTAGTGGAAACTGAGTTTTTACAACTTCAAGTCAATAAAGTAATAGGGAATGATTACGA

TGCCTTGATTAAGCTCTCTCTTCGCCACAGACCTGATTTACTCATTGTTGGTGAAATTAGGGACAGTCAAACGGCAAAAG

CTGTTCTCCGCGCAAGTTTGACGGGCTATACCGTGTTTTCTACTGTTCATGCACGCTCAATATCGGGTGTAATTGCACGG

CTAAAGGAACTTGGGTTAACTGATTGGGAGCTACAATCTAGCCTTCAGCGGGTGATTTATCAGCGCTTAATTGCAGGAAA

GGGGTTGCTTGTTTATGAAAAAGAAAAGTTTGAAGAATGGCAACCAGACGAATGGAATGGCCAGATTGATCAGTTGGTTG

CAGATGGATTTATCTCAGCTGTTACAGCTGCGTACGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-264)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Lactococcus lactis subsp. cremoris KW2	59.236	100	0.592
comYA	Streptococcus gordonii str. Challis substr. CH1	53.333	100	0.535
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	53.035	99.682	0.529
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	52.716	99.682	0.525
comGA/cglA/cilD	Streptococcus pneumoniae D39	52.716	99.682	0.525
comGA/cglA/cilD	Streptococcus pneumoniae R6	52.716	99.682	0.525
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	52.716	99.682	0.525
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	52.396	99.682	0.522
comYA	Streptococcus mutans UA159	51.282	99.363	0.51
comYA	Streptococcus mutans UA140	51.282	99.363	0.51

Detailed information NTG0794068