Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PUN47_RS13610 Genome accession   NZ_CP118599
Coordinates   2908031..2909767 (-) Length   578 a.a.
NCBI ID   WP_274888693.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 10M-VF     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2903031..2914767
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN47_RS13590 (PUN47_13590) - 2903733..2904563 (-) 831 WP_020329450.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  PUN47_RS13595 (PUN47_13595) - 2904658..2906166 (-) 1509 WP_274888691.1 AAA family ATPase -
  PUN47_RS13600 (PUN47_13600) aroB 2906212..2907297 (-) 1086 WP_075988075.1 3-dehydroquinate synthase -
  PUN47_RS13605 (PUN47_13605) aroK 2907325..2907843 (-) 519 WP_004728569.1 shikimate kinase AroK -
  PUN47_RS13610 (PUN47_13610) pilQ 2908031..2909767 (-) 1737 WP_274888693.1 type IV pilus secretin PilQ Machinery gene
  PUN47_RS13615 (PUN47_13615) pilP 2909792..2910304 (-) 513 WP_274888694.1 pilus assembly protein PilP Machinery gene
  PUN47_RS13620 (PUN47_13620) pilO 2910294..2910884 (-) 591 WP_222768516.1 type 4a pilus biogenesis protein PilO Machinery gene
  PUN47_RS13625 (PUN47_13625) pilN 2910877..2911467 (-) 591 WP_274888698.1 PilN domain-containing protein Machinery gene
  PUN47_RS13630 (PUN47_13630) pilM 2911457..2912461 (-) 1005 WP_274888699.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62482.41 Da        Isoelectric Point: 4.5110

>NTDB_id=793859 PUN47_RS13610 WP_274888693.1 2908031..2909767(-) (pilQ) [Vibrio fluvialis strain 10M-VF]
MIKGLNASTAWGIRCLFVGVCLLLSAATLAQEAAANQLKNIDFRINKDKAAVLIVELDSASAVVDVQKTEQGLNIELLKT
DVSDDKLYLLDVRDFATAVENIEVFRQQPSTLLVATVQGEFNYDYSLKGNYLEVVIRKLKAEEKAKPKSVLEKEGKLISI
NFQDIPVRNVLQLIADYNEFNLVVSDSVRGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDLREKQQLE
KARMAEELGDLQSDILIINFAKASDIAQMIGGDGAVNMLSERGSISIDERTNALLIRELPENIAVIRDIVASLDIPVKQV
QIEARIVTVNEGNLDELGVRWGFSSTNGSTTTGGSIESNLGAIGLYDGGNNSGGSGDGVAIDDFLNVNLAATSANASSIA
FQVAKLGADTLLDLELSAMQRESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPD
NRLVLDLSVTQDRPGEVVKTGTGEAVAINTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPVLGALFRRSYE
QMGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=793859 PUN47_RS13610 WP_274888693.1 2908031..2909767(-) (pilQ) [Vibrio fluvialis strain 10M-VF]
ATGATTAAAGGACTAAATGCCTCCACGGCGTGGGGAATACGTTGTCTGTTTGTGGGCGTATGCCTGCTGTTATCTGCCGC
CACACTTGCCCAGGAAGCGGCGGCCAATCAGCTGAAAAATATCGATTTTCGCATCAATAAAGATAAAGCGGCGGTATTGA
TTGTCGAACTGGATTCCGCCTCGGCCGTGGTGGATGTGCAGAAAACCGAGCAAGGCCTGAATATCGAACTGCTGAAAACC
GATGTCAGCGACGACAAACTCTACCTGCTTGATGTGCGCGACTTTGCCACCGCAGTGGAAAACATCGAAGTGTTCCGCCA
GCAGCCCAGCACCTTGTTGGTCGCAACGGTGCAGGGGGAGTTCAATTATGATTACAGCCTGAAGGGTAACTACCTGGAAG
TGGTGATCCGCAAGCTAAAAGCCGAGGAGAAAGCCAAACCAAAAAGCGTATTAGAAAAAGAAGGCAAGCTGATTTCGATT
AACTTTCAGGATATTCCGGTGCGCAATGTGCTGCAGTTGATCGCAGACTACAATGAGTTCAATTTGGTAGTTTCCGATTC
AGTGCGCGGTAATCTGACGCTGCGACTTGATGGTGTGCCCTGGCAGCAGGTGCTGGACATTATTCTGCAAGTCAAAGGTC
TGGATAAACGCGTCGACGGCAATGTGATTCTGGTGGCGCCCAAAGAAGAGCTGGATCTGCGTGAAAAGCAGCAGTTGGAA
AAAGCCCGCATGGCGGAAGAACTCGGCGATCTGCAATCGGATATTCTCATCATCAACTTTGCCAAAGCGTCTGATATTGC
ACAGATGATTGGCGGCGATGGGGCGGTCAATATGTTGTCGGAGCGTGGCTCGATCAGCATTGATGAACGCACCAACGCGC
TGCTGATTCGTGAGCTGCCGGAGAACATTGCTGTGATTCGCGATATCGTGGCGTCGCTCGATATTCCGGTCAAGCAAGTA
CAGATTGAAGCACGTATTGTGACGGTCAATGAAGGCAACTTGGATGAACTGGGAGTGCGCTGGGGTTTCAGTTCCACCAA
TGGCAGCACGACCACGGGTGGCTCGATTGAAAGCAATCTGGGGGCGATTGGTTTGTACGATGGCGGTAATAATAGTGGCG
GTTCTGGTGATGGCGTTGCGATTGATGATTTCCTTAACGTTAATCTCGCAGCCACCTCAGCTAATGCGTCCAGCATTGCG
TTTCAGGTCGCCAAGCTCGGCGCCGATACCTTGCTCGATCTTGAACTGTCAGCCATGCAACGTGAGTCGAAAGCGGAAAT
CATCTCCAGTCCGAGGCTGATCACTACCAACAAGCAGCCGGCGTATATTGAGCAGGGTACTGAGATTCCGTACCTGGAAT
CCTCATCCAGTGGCGCCACCAGTGTGGCATTCAAGAAGGCAGTACTCAGCCTCAAAGTGACGCCGCAAATCACCCCCGAT
AACCGTCTGGTGTTGGATTTGAGCGTCACTCAGGATCGGCCGGGTGAAGTGGTGAAAACCGGTACTGGCGAGGCGGTGGC
GATCAACACGCAGCGCATTGGTACACAGGTGCTGGTCAACAACGGTGAAACCGTGGTTCTGGGCGGCATTTTCCAGCACA
GCATTACGAATTCAGTCGACAAGGTGCCGCTGCTGGGTGACTTGCCCGTGTTGGGCGCGCTGTTCCGTCGCAGCTACGAA
CAAATGGGCAAAAGTGAGCTGCTGATCTTCGTAACACCGAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(418-576)

Bacterial type II/III secretion system short domain (Secretin_N)

(258-319)

Secretin and TonB N terminus short domain (STN)

(180-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

81.52

100

0.817
  pilQ Vibrio cholerae strain A1552

81.52

100

0.817
  pilQ Vibrio campbellii strain DS40M4

79.235

94.983

0.753
  pilQ Legionella pneumophila strain ERS1305867

42.364

95.156

0.403
  pilQ Legionella pneumophila strain Lp02

42.364

95.156

0.403
  pilQ Pseudomonas aeruginosa PAK

40.721

96.021

0.391
  comQ Acinetobacter baylyi ADP1

37.973

100

0.382