Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PUN47_RS13620 Genome accession   NZ_CP118599
Coordinates   2910294..2910884 (-) Length   196 a.a.
NCBI ID   WP_222768516.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 10M-VF     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2905294..2915884
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN47_RS13600 (PUN47_13600) aroB 2906212..2907297 (-) 1086 WP_075988075.1 3-dehydroquinate synthase -
  PUN47_RS13605 (PUN47_13605) aroK 2907325..2907843 (-) 519 WP_004728569.1 shikimate kinase AroK -
  PUN47_RS13610 (PUN47_13610) pilQ 2908031..2909767 (-) 1737 WP_274888693.1 type IV pilus secretin PilQ Machinery gene
  PUN47_RS13615 (PUN47_13615) pilP 2909792..2910304 (-) 513 WP_274888694.1 pilus assembly protein PilP Machinery gene
  PUN47_RS13620 (PUN47_13620) pilO 2910294..2910884 (-) 591 WP_222768516.1 type 4a pilus biogenesis protein PilO Machinery gene
  PUN47_RS13625 (PUN47_13625) pilN 2910877..2911467 (-) 591 WP_274888698.1 PilN domain-containing protein Machinery gene
  PUN47_RS13630 (PUN47_13630) pilM 2911457..2912461 (-) 1005 WP_274888699.1 type IV pilus assembly protein PilM Machinery gene
  PUN47_RS13635 (PUN47_13635) - 2912604..2915084 (+) 2481 WP_032081685.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22681.10 Da        Isoelectric Point: 4.8135

>NTDB_id=793861 PUN47_RS13620 WP_222768516.1 2910294..2910884(-) (pilO) [Vibrio fluvialis strain 10M-VF]
MANLQELELDEIAEWPLLPQLAVLLLLVLALQGAGYWLHMLPKQDALEGLKQQEQTLKSTIRIKASKVATLPKLKIQLDE
LAERYDFLLRQLPVQKELASMLASVNELGLENTLTFTRIDWGEKQNQEFLYRLPLNIELTGNYHSIGDFSEAIARLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKPEVSDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=793861 PUN47_RS13620 WP_222768516.1 2910294..2910884(-) (pilO) [Vibrio fluvialis strain 10M-VF]
ATGGCTAACTTGCAGGAACTGGAACTGGATGAAATCGCTGAATGGCCTTTGTTGCCTCAGTTGGCCGTGTTGTTACTGCT
GGTTCTGGCACTGCAAGGCGCGGGCTATTGGTTACATATGTTGCCCAAGCAGGATGCGCTCGAAGGTTTAAAACAGCAGG
AACAAACGCTCAAATCGACGATACGTATCAAAGCCAGCAAAGTGGCCACGTTACCCAAACTCAAAATCCAACTGGATGAA
CTGGCAGAACGGTATGACTTCCTGCTGCGTCAACTGCCGGTGCAAAAAGAGCTTGCCAGTATGCTGGCGTCGGTTAACGA
GCTGGGGCTGGAAAATACGCTGACGTTTACGCGGATTGACTGGGGGGAGAAGCAGAATCAGGAATTCCTCTATCGTCTGC
CACTCAATATCGAACTGACCGGCAATTATCACAGCATCGGCGACTTTTCCGAAGCGATTGCGCGCTTGCCACGCATCATC
AACTTTGACGATGTCGATTGGCAGCGCGTCAGTCAGGAGAGCAGCACGCTGCACTTTCGAGTTCGCGCTTACACCTATCA
GTTTAAACCGGAGGTGAGTGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

79.082

100

0.791
  pilO Vibrio campbellii strain DS40M4

68.421

96.939

0.663