Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PWW32_RS16645 Genome accession   NZ_CP118580
Coordinates   3529497..3530720 (+) Length   407 a.a.
NCBI ID   WP_005446256.1    Uniprot ID   K5TX94
Organism   Vibrio harveyi strain fish3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3524497..3535720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW32_RS16625 ampD 3525515..3526066 (-) 552 WP_154203416.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PWW32_RS16630 nadC 3526159..3527046 (+) 888 WP_199449848.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PWW32_RS16635 - 3527311..3527778 (+) 468 WP_050933475.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PWW32_RS16640 pilB 3527778..3529463 (+) 1686 WP_005446257.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PWW32_RS16645 pilC 3529497..3530720 (+) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  PWW32_RS16650 pilD 3530793..3531662 (+) 870 WP_045488712.1 A24 family peptidase Machinery gene
  PWW32_RS16655 coaE 3531663..3532277 (+) 615 WP_050922069.1 dephospho-CoA kinase -
  PWW32_RS16660 zapD 3532305..3533045 (+) 741 WP_038897986.1 cell division protein ZapD -
  PWW32_RS16665 yacG 3533102..3533302 (+) 201 WP_026000212.1 DNA gyrase inhibitor YacG -
  PWW32_RS16670 rplS 3533868..3534221 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  PWW32_RS16675 trmD 3534263..3535006 (-) 744 WP_005446247.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  PWW32_RS16680 rimM 3535034..3535582 (-) 549 WP_005446245.1 ribosome maturation factor RimM -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44945.20 Da        Isoelectric Point: 10.3829

>NTDB_id=793580 PWW32_RS16645 WP_005446256.1 3529497..3530720(+) (pilC) [Vibrio harveyi strain fish3]
MKTIAPQLKNYRWKGINSSGKKTSGNVLAMTEVEVRERLDAQHIKIKKLKKGSISFITKISHRVKGKDITVFTRQIATML
MTGVPIVQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASTHFDPLYTDLIATGEQSGNLSQVFERLATYREK
SEQLRAKVIKALIYPAMVVLVALGVSYLMLTKVIPEFEKMFTGFGAELPAFTQMVLNLSAWTQNWGPFIGLSFVSLLISG
KIMSQRSDSFRLLTARSSLRVPILGPVLSKAAIAKFSRTLATSFSAGIPILTALKTTSKTSGNMHYQLAIEEVYRDTAAG
MPMYIAMRNCNVFPELVLQMVMIGEESGRLDDMLNKIAAIYEFEVDNTVDNLSKILEPLIIVFLGVVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=793580 PWW32_RS16645 WP_005446256.1 3529497..3530720(+) (pilC) [Vibrio harveyi strain fish3]
ATGAAAACCATCGCTCCACAACTTAAGAACTACCGTTGGAAAGGCATTAATAGCTCGGGTAAAAAAACCTCTGGTAATGT
GCTCGCAATGACAGAAGTCGAGGTAAGAGAGCGCCTTGATGCTCAGCACATCAAGATAAAAAAACTCAAAAAAGGCAGTA
TTTCTTTTATCACCAAGATAAGTCATCGAGTAAAAGGCAAAGACATTACGGTGTTTACTCGTCAAATCGCCACCATGTTG
ATGACTGGCGTCCCTATTGTTCAGGCACTCAAGTTAGTTTCAGAGAACCATAAAAAAGCTGAAATGAAGTCGATTCTAAT
GAGTGTGACGCGAGCGGTAGAAGCAGGGACACCGATGTCGAAAGCCATGCGTACCGCTAGCACGCACTTTGATCCACTTT
ACACCGACTTAATAGCAACAGGTGAGCAGTCCGGTAACTTATCCCAAGTCTTTGAGCGTCTGGCAACCTACCGAGAAAAA
AGCGAGCAGTTGCGTGCAAAGGTTATTAAAGCGCTCATTTACCCGGCGATGGTGGTATTAGTAGCACTTGGGGTTTCTTA
TTTAATGCTGACAAAGGTTATTCCTGAGTTTGAAAAAATGTTTACAGGTTTTGGTGCCGAATTACCCGCCTTCACCCAGA
TGGTACTCAACCTTTCTGCTTGGACCCAAAATTGGGGTCCCTTCATTGGGCTGAGCTTTGTGAGCTTACTCATTTCTGGC
AAAATCATGTCACAACGCTCCGACTCATTTCGTCTTTTGACTGCACGGTCGAGTTTAAGAGTACCTATTTTAGGACCAGT
TTTATCCAAAGCTGCCATTGCGAAGTTTAGCCGTACTTTGGCGACCAGTTTTAGCGCAGGTATTCCTATCCTAACCGCCT
TGAAAACTACCTCAAAAACCTCTGGTAACATGCACTATCAATTGGCAATCGAAGAGGTATATCGAGATACTGCCGCTGGT
ATGCCAATGTACATTGCCATGCGTAACTGTAACGTCTTCCCAGAATTGGTATTGCAAATGGTCATGATTGGTGAAGAGTC
AGGTCGCTTAGATGACATGCTCAACAAGATAGCCGCTATTTATGAGTTCGAAGTCGATAACACCGTCGACAACCTAAGTA
AGATTTTGGAACCGTTGATCATTGTTTTTCTGGGTGTCGTTGTCGGTGGTTTGGTGACAGCAATGTACTTACCAATCTTT
AACTTGATGAGCGTATTGGGATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K5TX94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

98.28

100

0.983
  pilC Vibrio cholerae strain A1552

74

98.28

0.727
  pilC Acinetobacter baylyi ADP1

42.462

97.789

0.415
  pilC Pseudomonas stutzeri DSM 10701

41.414

97.297

0.403
  pilC Acinetobacter baumannii D1279779

40.447

99.017

0.4
  pilG Neisseria gonorrhoeae MS11

39.558

100

0.396
  pilC Legionella pneumophila strain ERS1305867

39.163

99.754

0.391
  pilG Neisseria meningitidis 44/76-A

38.725

100

0.388