Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PWA59_RS13255 Genome accession   NZ_CP118541
Coordinates   2665595..2666665 (-) Length   356 a.a.
NCBI ID   WP_014418378.1    Uniprot ID   A0AAP4DGV9
Organism   Bacillus velezensis strain HC-5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2660595..2671665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWA59_RS13205 (PWA59_13205) - 2660654..2661238 (-) 585 WP_014418370.1 signal peptidase I -
  PWA59_RS13210 (PWA59_13210) tapA 2661210..2661881 (-) 672 WP_014418371.1 amyloid fiber anchoring/assembly protein TapA -
  PWA59_RS13215 (PWA59_13215) - 2662140..2662469 (+) 330 WP_014418372.1 DUF3889 domain-containing protein -
  PWA59_RS13220 (PWA59_13220) - 2662510..2662689 (-) 180 WP_003153093.1 YqzE family protein -
  PWA59_RS13225 (PWA59_13225) comGG 2662746..2663123 (-) 378 WP_014418373.1 competence type IV pilus minor pilin ComGG Machinery gene
  PWA59_RS13230 (PWA59_13230) comGF 2663124..2663519 (-) 396 WP_014721501.1 competence type IV pilus minor pilin ComGF -
  PWA59_RS13235 (PWA59_13235) comGE 2663533..2663847 (-) 315 WP_032863566.1 competence type IV pilus minor pilin ComGE Machinery gene
  PWA59_RS13240 (PWA59_13240) comGD 2663831..2664268 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  PWA59_RS13245 (PWA59_13245) comGC 2664258..2664566 (-) 309 WP_014418376.1 competence type IV pilus major pilin ComGC Machinery gene
  PWA59_RS13250 (PWA59_13250) comGB 2664571..2665608 (-) 1038 WP_014418377.1 competence type IV pilus assembly protein ComGB Machinery gene
  PWA59_RS13255 (PWA59_13255) comGA 2665595..2666665 (-) 1071 WP_014418378.1 competence type IV pilus ATPase ComGA Machinery gene
  PWA59_RS13260 (PWA59_13260) - 2666858..2667808 (-) 951 WP_014418379.1 magnesium transporter CorA family protein -
  PWA59_RS13265 (PWA59_13265) - 2667953..2669254 (+) 1302 WP_029973873.1 hemolysin family protein -
  PWA59_RS13270 (PWA59_13270) - 2669292..2670122 (-) 831 WP_014418381.1 STAS domain-containing protein -
  PWA59_RS13280 (PWA59_13280) - 2670329..2670709 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  PWA59_RS13285 (PWA59_13285) - 2670945..2671190 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.81 Da        Isoelectric Point: 9.3485

>NTDB_id=793114 PWA59_RS13255 WP_014418378.1 2665595..2666665(-) (comGA) [Bacillus velezensis strain HC-5]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKQQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=793114 PWA59_RS13255 WP_014418378.1 2665595..2666665(-) (comGA) [Bacillus velezensis strain HC-5]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCAGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCAATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374