Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   PWA59_RS13250 Genome accession   NZ_CP118541
Coordinates   2664571..2665608 (-) Length   345 a.a.
NCBI ID   WP_014418377.1    Uniprot ID   -
Organism   Bacillus velezensis strain HC-5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2659571..2670608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWA59_RS13200 (PWA59_13200) - 2659804..2660589 (-) 786 WP_007408329.1 TasA family protein -
  PWA59_RS13205 (PWA59_13205) - 2660654..2661238 (-) 585 WP_014418370.1 signal peptidase I -
  PWA59_RS13210 (PWA59_13210) tapA 2661210..2661881 (-) 672 WP_014418371.1 amyloid fiber anchoring/assembly protein TapA -
  PWA59_RS13215 (PWA59_13215) - 2662140..2662469 (+) 330 WP_014418372.1 DUF3889 domain-containing protein -
  PWA59_RS13220 (PWA59_13220) - 2662510..2662689 (-) 180 WP_003153093.1 YqzE family protein -
  PWA59_RS13225 (PWA59_13225) comGG 2662746..2663123 (-) 378 WP_014418373.1 competence type IV pilus minor pilin ComGG Machinery gene
  PWA59_RS13230 (PWA59_13230) comGF 2663124..2663519 (-) 396 WP_014721501.1 competence type IV pilus minor pilin ComGF -
  PWA59_RS13235 (PWA59_13235) comGE 2663533..2663847 (-) 315 WP_032863566.1 competence type IV pilus minor pilin ComGE Machinery gene
  PWA59_RS13240 (PWA59_13240) comGD 2663831..2664268 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  PWA59_RS13245 (PWA59_13245) comGC 2664258..2664566 (-) 309 WP_014418376.1 competence type IV pilus major pilin ComGC Machinery gene
  PWA59_RS13250 (PWA59_13250) comGB 2664571..2665608 (-) 1038 WP_014418377.1 competence type IV pilus assembly protein ComGB Machinery gene
  PWA59_RS13255 (PWA59_13255) comGA 2665595..2666665 (-) 1071 WP_014418378.1 competence type IV pilus ATPase ComGA Machinery gene
  PWA59_RS13260 (PWA59_13260) - 2666858..2667808 (-) 951 WP_014418379.1 magnesium transporter CorA family protein -
  PWA59_RS13265 (PWA59_13265) - 2667953..2669254 (+) 1302 WP_029973873.1 hemolysin family protein -
  PWA59_RS13270 (PWA59_13270) - 2669292..2670122 (-) 831 WP_014418381.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39600.91 Da        Isoelectric Point: 9.6787

>NTDB_id=793113 PWA59_RS13250 WP_014418377.1 2664571..2665608(-) (comGB) [Bacillus velezensis strain HC-5]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLKLQLHKRQLAELTDGIRRLREGDAFYQVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGELLQRKLMQTNQIKKMLRYPMFLISSVCVMFYILQSLIIPQFSGIYQSMNMNTSGATAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPCYEKDLAAAVSHGQANGRLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=793113 PWA59_RS13250 WP_014418377.1 2664571..2665608(-) (comGB) [Bacillus velezensis strain HC-5]
ATGAAGCGGATTAAACGAACCTGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGTCTGATTGAAGGGCTAAGACTGCTGAAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGACGGACGGAATCC
GCCGGTTGAGGGAAGGTGATGCGTTTTACCAAGTGCTTGAGGCACTGTCTTTTCACAAAGAAGCCGTCTCTATCTGCTAT
TTCGCTGAGAAACACGGGGAATTGCCGGGGGCGATGAAACAAAGCGGCGAATTATTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATTTCTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TCATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACCTCCGGTGCGACTGCTTTCATCTTTGCATTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTGTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAGAA
AAAATCCCCGCAAGATAAAATGCTGATTGTTGTGAAAATACCACTATTAGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTTTCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGTGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
CGGGCATCCGTGCTATGAAAAAGACCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCGGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

66.254

93.623

0.62