Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PVT67_RS02295 Genome accession   NZ_CP118480
Coordinates   481556..483271 (+) Length   571 a.a.
NCBI ID   WP_301497400.1    Uniprot ID   -
Organism   Gallaecimonas kandeliae strain Q10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 476556..488271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVT67_RS02270 (PVT67_02270) - 476854..477378 (+) 525 WP_301497390.1 cytochrome b -
  PVT67_RS02275 (PVT67_02275) - 477462..478934 (+) 1473 WP_301497392.1 SulP family inorganic anion transporter -
  PVT67_RS02280 (PVT67_02280) - 478945..479793 (+) 849 WP_301497394.1 universal stress protein -
  PVT67_RS02285 (PVT67_02285) nadC 479964..480839 (-) 876 WP_336407793.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PVT67_RS02290 (PVT67_02290) - 481094..481498 (+) 405 WP_301497398.1 pilin -
  PVT67_RS02295 (PVT67_02295) pilB 481556..483271 (+) 1716 WP_301497400.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PVT67_RS02300 (PVT67_02300) pilC 483281..484522 (+) 1242 WP_301497402.1 type II secretion system F family protein Machinery gene
  PVT67_RS02305 (PVT67_02305) pilD 484528..485391 (+) 864 WP_301497404.1 prepilin peptidase Machinery gene
  PVT67_RS02310 (PVT67_02310) coaE 485388..485990 (+) 603 WP_301497406.1 dephospho-CoA kinase -
  PVT67_RS02315 (PVT67_02315) zapD 486022..486759 (+) 738 WP_301497408.1 cell division protein ZapD -
  PVT67_RS02320 (PVT67_02320) yacG 486769..486942 (+) 174 WP_301497410.1 DNA gyrase inhibitor YacG -
  PVT67_RS02325 (PVT67_02325) ampD 487002..487544 (+) 543 WP_301497412.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62999.59 Da        Isoelectric Point: 6.6265

>NTDB_id=792617 PVT67_RS02295 WP_301497400.1 481556..483271(+) (pilB) [Gallaecimonas kandeliae strain Q10]
MSGFLPVAKGTLALLLRRGKITQAKAEELLKENHSGLLPLFDVLKKSELSSEAFAGFLSEQYGLPIFDLSKFDLDTIPST
LLNEQLIQKHSVLPLFKRQNRIFIATADPSNQSALEDFRFNLRLPTEPVLVNADELAKAIDKLLERTGGNMLDVGDVKDS
ELADLVVDDSEDRDQDLSATGKEDDAPIVIYVNKLLMDAIRKGASDLHFEPYEKSYRVRFRIDGILHEVAAPPVSVANRL
AARIKVMSKLDIAERRIPQDGRIKLRLSRKKAIDFRVSTLPTLWGEKIVMRILDSSSAQLGIDMLGYEPDQKKMYLEALA
KPQGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLPGVNQVQIHAKAGMTFASALRAFLRQDPDVIMVGE
IRDLETAEISIKAAQTGHLVLSTLHTNSASETLTRLMNMGVPPYNIASSVTLVIAQRLGRRLCPVCKQAEQLPKDELFRQ
GFSPKQIEEGFTLFRPVGCSECTGGYKGRVGIYETMPMTEAIAKIIMEGGNSLDIAAQAQREGVRNLRQAGLEKARQGVT
SLAEVNRITSY

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=792617 PVT67_RS02295 WP_301497400.1 481556..483271(+) (pilB) [Gallaecimonas kandeliae strain Q10]
ATGTCCGGATTCTTACCTGTTGCCAAAGGCACCCTTGCCCTGCTGCTTAGGCGAGGCAAGATCACCCAGGCCAAGGCCGA
GGAACTGCTTAAGGAAAACCATTCCGGGCTGCTTCCCCTCTTCGACGTCCTCAAGAAATCCGAGCTGAGCAGCGAGGCCT
TTGCCGGTTTCCTCTCAGAGCAGTACGGCTTGCCCATCTTCGACTTGAGCAAGTTCGACCTCGACACCATCCCCAGTACG
CTTTTGAATGAGCAGCTTATTCAAAAGCATTCTGTGCTGCCGCTCTTCAAGCGCCAAAACCGTATCTTTATCGCTACCGC
CGATCCCAGCAACCAGTCGGCCCTGGAAGATTTCCGTTTCAACCTACGTCTGCCAACAGAGCCCGTGTTGGTCAATGCCG
ACGAACTGGCAAAGGCCATCGACAAGCTCTTGGAGCGGACCGGCGGCAACATGCTGGACGTGGGGGACGTCAAGGACTCC
GAGCTGGCGGACCTGGTGGTGGATGACAGCGAGGACAGGGACCAGGACCTCAGCGCCACAGGCAAGGAAGACGATGCCCC
TATCGTCATCTATGTCAATAAATTGCTGATGGATGCCATCCGCAAAGGCGCCTCTGACCTTCACTTCGAACCCTACGAGA
AGAGCTACCGGGTCCGCTTTCGCATCGACGGTATCTTGCATGAAGTGGCGGCGCCTCCCGTATCCGTTGCCAACCGGCTG
GCAGCCCGCATCAAGGTCATGTCCAAGCTCGACATCGCCGAGCGGCGTATTCCACAGGATGGCCGTATCAAACTGCGCCT
GAGTCGCAAGAAGGCCATCGATTTCCGCGTCTCGACCCTACCCACCCTCTGGGGTGAGAAGATCGTCATGCGTATCCTCG
ACTCCAGCTCGGCACAGCTCGGTATCGACATGCTGGGCTACGAGCCGGACCAAAAGAAAATGTACCTCGAGGCCCTTGCC
AAGCCCCAGGGCATGATACTGGTGACGGGCCCTACAGGGTCAGGTAAGACGGTCTCCCTTTATACGGGCCTCAATATCCT
CAACACCGAGGAACGCAACATCTCCACGGCCGAAGATCCGGTGGAGATCAACCTGCCCGGGGTCAACCAAGTGCAGATCC
ACGCCAAGGCCGGTATGACCTTCGCCTCTGCCCTACGGGCTTTTTTGCGCCAGGATCCTGACGTCATCATGGTAGGGGAA
ATAAGGGACCTGGAAACGGCGGAGATCTCCATCAAGGCGGCCCAGACAGGCCACCTGGTGCTCTCGACCCTGCATACCAA
CTCGGCATCAGAGACCCTGACCCGTTTGATGAACATGGGGGTGCCGCCATACAACATCGCCTCCAGCGTGACCCTGGTCA
TAGCCCAGCGCCTTGGACGCCGGCTCTGTCCCGTTTGCAAGCAGGCGGAGCAACTACCGAAAGATGAGCTGTTCCGCCAA
GGCTTCAGCCCCAAGCAGATCGAAGAAGGCTTTACCCTCTTCCGTCCTGTAGGCTGCAGCGAGTGCACAGGCGGCTACAA
GGGAAGGGTGGGTATCTACGAGACCATGCCCATGACCGAAGCCATCGCCAAGATCATCATGGAGGGAGGCAACTCCCTGG
ACATCGCCGCCCAGGCCCAGCGGGAAGGGGTCAGGAACCTGCGCCAGGCCGGTTTGGAAAAGGCCCGGCAGGGGGTGACT
TCCCTGGCCGAGGTTAATCGCATCACCAGCTACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

56.131

100

0.569
  pilB Acinetobacter baumannii D1279779

59.811

92.82

0.555
  pilB Legionella pneumophila strain ERS1305867

54.514

100

0.55
  pilB Vibrio cholerae strain A1552

53.086

99.299

0.527
  pilB Vibrio parahaemolyticus RIMD 2210633

51.957

98.424

0.511
  pilB Vibrio campbellii strain DS40M4

51.418

98.774

0.508
  pilF Neisseria gonorrhoeae MS11

48.148

99.299

0.478
  pilF Thermus thermophilus HB27

39.683

99.299

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.293

95.622

0.385
  pilB/pilB1 Synechocystis sp. PCC 6803

43.093

84.939

0.366