Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PVK35_RS03330 Genome accession   NZ_CP118442
Coordinates   482970..484676 (-) Length   568 a.a.
NCBI ID   WP_274799055.1    Uniprot ID   -
Organism   Aeromonas caviae strain CYEY0630     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 477970..489676
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVK35_RS03300 (PVK35_03300) mutT 478588..478992 (+) 405 WP_191967205.1 8-oxo-dGTP diphosphatase MutT -
  PVK35_RS03305 (PVK35_03305) yacG 479050..479244 (-) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  PVK35_RS03310 (PVK35_03310) zapD 479255..479977 (-) 723 WP_010675825.1 cell division protein ZapD -
  PVK35_RS03315 (PVK35_03315) coaE 480015..480629 (-) 615 WP_239054132.1 dephospho-CoA kinase -
  PVK35_RS03320 (PVK35_03320) pilD 480648..481523 (-) 876 WP_239054131.1 A24 family peptidase Machinery gene
  PVK35_RS03325 (PVK35_03325) pilC 481601..482842 (-) 1242 WP_274799054.1 type II secretion system F family protein Machinery gene
  PVK35_RS03330 (PVK35_03330) pilB 482970..484676 (-) 1707 WP_274799055.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  PVK35_RS03335 (PVK35_03335) - 484680..485120 (-) 441 WP_274799056.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PVK35_RS03340 (PVK35_03340) nadC 485431..486294 (-) 864 WP_128296228.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PVK35_RS03345 (PVK35_03345) - 486298..486768 (-) 471 WP_010675818.1 retropepsin-like aspartic protease -
  PVK35_RS03350 (PVK35_03350) ampD 486897..487469 (+) 573 WP_239054130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PVK35_RS03355 (PVK35_03355) pdhR 487746..488510 (+) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62566.95 Da        Isoelectric Point: 5.8214

>NTDB_id=792592 PVK35_RS03330 WP_274799055.1 482970..484676(-) (pilB) [Aeromonas caviae strain CYEY0630]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQAKAQRKPFVTFLIENDIIDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRVDGILHEVATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKTQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=792592 PVK35_RS03330 WP_274799055.1 482970..484676(-) (pilB) [Aeromonas caviae strain CYEY0630]
ATGACCTCTAGCCTCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCTTCCCTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCCAAGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATTGAAAATGACATCATCGACAGCAAAGCCCTCG
CCGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTGGCGGCCTTCGATCTCGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAGCACCATGTCCTGCCCATCTACACCCAGGGGCATACCCTCTATATCGCCATGTCCGA
TCCGACCAATGTGTCGGCTCTGGAGGATTTTGGCTTCAGTTTTGGCCTACACACGGAAGCCCTGCTGGTCGAAGAGAGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGAGCAGGATACCCTAGGCATGGATCATATCGACGAGGCCGAGATC
TCCGAACTCGAGGTCTCCGACGAGAGTTCCCGCCTCGACGAGAGCGTCAACACCGCGGACGATGACGCCCCCATCGTCAA
ATACATCCACAAGATCATGATGGATGCCATCAAGCGCGGGGCCTCCGACCTGCATTTCGAACCCTACGAGACCAAGTACC
GGATCCGCTTTCGGGTAGACGGCATACTGCACGAAGTCGCCACGCCGCCGGTCAACCTGGCCAACCGCTTCGCGGCTCGT
CTCAAGGTGATGGCGCGGCTCGACATTGCCGAACGGCGCCTGCCCCAGGATGGCCGGATCAAGCTCAAGATCTCACGCAA
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTGATCCGACTGCTGGACTCCTCGG
CCGCCCGGCTCAATATCGATCAGCTCGGCTTTGACGACCGGCAGAAGACGCAATACCTGCACGCCCTCTCCAAACCTCAG
GGCATGATCCTGGTGACCGGCCCGACGGGGTCAGGCAAGACGGTCTCACTCTATACCGGCCTCAACATCCTCAACACCAG
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTCGAGATAAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTACGCCAGGATCCCGACATCGTCATGGTGGGGGAGATACGGGAT
TTGGAAACCGCCGAGATTGCTATCAAGGCCGCCCAGACCGGTCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTCACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCTTCGGTCACCCTCATCATGGCCCAGC
GCCTTGCCCGCAAACTCTGCGATCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTGGGCTTCACC
CAGCAGCAACTGGCCGCCGGGTTGCGGCTGTTCAAGCCCGTGGGATGCAAGGAGTGCTCGGGAGGCTACAGAGGCCGGGT
CGGCATCTATGAGATCATGCTGATGTCCGACAACATCGCCAAACTCATCATGCAGGGAGCCAACTCGCTGCAGATTGCCG
CGATAGCCCAAAAAGAAGGGATGCGCACCCTGCGTACATCCGGCCTCGAGAAAGCGCGTCTTGGCGTCACCAGCCTGGCC
GAAATCAACAGGATCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.24

99.12

0.548
  pilB Acinetobacter baumannii D1279779

54.85

99.824

0.548
  pilB Legionella pneumophila strain ERS1305867

54.69

99.472

0.544
  pilB Vibrio cholerae strain A1552

53.415

100

0.537
  pilB Vibrio campbellii strain DS40M4

50.53

99.648

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

50.089

99.12

0.496
  pilF Neisseria gonorrhoeae MS11

46.737

99.824

0.467
  pilF Thermus thermophilus HB27

39.962

92.958

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

37.975

97.359

0.37