Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PUV55_RS07390 Genome accession   NZ_CP118165
Coordinates   1494478..1495863 (+) Length   461 a.a.
NCBI ID   WP_160429046.1    Uniprot ID   -
Organism   Glaesserella parasuis strain XP11     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1489478..1500863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUV55_RS07370 (PUV55_07370) - 1490683..1491654 (+) 972 WP_012621529.1 IS110 family transposase -
  PUV55_RS07375 (PUV55_07375) gmk 1491723..1492349 (+) 627 WP_035491622.1 guanylate kinase -
  PUV55_RS07380 (PUV55_07380) radA 1492402..1493778 (-) 1377 WP_274358630.1 DNA repair protein RadA -
  PUV55_RS07385 (PUV55_07385) pilA 1493966..1494430 (+) 465 WP_005711781.1 pilin Machinery gene
  PUV55_RS07390 (PUV55_07390) pilB 1494478..1495863 (+) 1386 WP_160429046.1 GspE/PulE family protein Machinery gene
  PUV55_RS07395 (PUV55_07395) pilC 1495856..1497052 (+) 1197 WP_274358631.1 type II secretion system F family protein Machinery gene
  PUV55_RS07400 (PUV55_07400) - 1497049..1497717 (+) 669 WP_005711775.1 prepilin peptidase -
  PUV55_RS07405 (PUV55_07405) dapE 1497801..1498934 (+) 1134 WP_005711772.1 succinyl-diaminopimelate desuccinylase -
  PUV55_RS07410 (PUV55_07410) clpX 1499008..1500258 (-) 1251 WP_160429045.1 ATP-dependent protease ATP-binding subunit ClpX -
  PUV55_RS07415 (PUV55_07415) clpP 1500265..1500846 (-) 582 WP_010787003.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52663.43 Da        Isoelectric Point: 5.1467

>NTDB_id=790908 PUV55_RS07390 WP_160429046.1 1494478..1495863(+) (pilB) [Glaesserella parasuis strain XP11]
MSQYTICEQQTERIFDISPSRWQKNCEEKQLLLRYLAVPVQETEHKLWLAVDDENNLTACEIFAFMTHKQIEPVVIASDE
LKYLLNALSPEQQPLYEESELAFAEQEQEQLNLSDPIIQLLDNLFKFCLAQNASDIHIEPRKQKLIIRLRIDGVLHLYKS
LSIQLASRLISRIKLLAKLDISELRQPQDGQFSFTTALADTLDFRVSSLPTIYGEKLVLRLQKNKPTSFDFLQLGFNPQQ
QTTLLNALKQPQGLILVTGPTGSGKSITLYSALNYLNQMDKHILTAEDPVEIEIDGLIQTQVNQSINLDFSQLLRTFLRQ
DPDIIMLGEIRDEESAKMALRASQTGHLVLSTLHTNDALSAVERLLQLGIQEYEIQNSLLLVIAQRLVRKLCKKCRGTGC
DECYQGYKGRIGIYQCLSRTAKKFDKQTAYLDFSSLRESAKQKIEQQLTNEIEVDRVLGDE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=790908 PUV55_RS07390 WP_160429046.1 1494478..1495863(+) (pilB) [Glaesserella parasuis strain XP11]
ATGTCCCAATACACTATCTGCGAACAACAAACCGAACGCATTTTTGATATATCGCCTAGCCGTTGGCAAAAAAATTGCGA
GGAGAAACAGCTTTTATTGCGTTACCTTGCCGTTCCTGTGCAAGAAACTGAGCATAAATTATGGCTTGCCGTTGATGATG
AAAACAATCTCACCGCTTGCGAAATCTTTGCTTTTATGACCCATAAGCAGATTGAGCCTGTGGTGATTGCTTCTGATGAA
CTTAAATACTTGCTTAACGCACTCTCGCCTGAGCAACAACCCCTTTATGAAGAGAGCGAACTGGCTTTCGCAGAACAAGA
GCAAGAACAGCTCAACTTAAGCGATCCGATTATTCAACTGCTCGATAATCTTTTTAAATTCTGTTTAGCCCAAAATGCGT
CCGACATTCATATTGAACCACGCAAGCAAAAATTGATTATTCGCCTACGCATTGACGGTGTTTTGCACCTGTATAAAAGC
CTGTCTATTCAACTGGCAAGCCGACTTATTTCACGGATAAAACTGCTGGCGAAGCTAGATATTAGTGAATTACGCCAGCC
ACAAGATGGGCAATTTAGCTTTACTACCGCCTTGGCGGACACCCTTGATTTTCGGGTATCTAGCCTGCCGACGATCTACG
GCGAAAAACTGGTATTGCGCCTACAAAAAAACAAACCAACCAGTTTTGATTTTCTGCAACTTGGCTTTAATCCACAACAG
CAGACCACACTACTCAACGCCTTGAAACAACCGCAAGGGCTGATTTTAGTGACAGGACCAACGGGCAGTGGCAAAAGTAT
CACCCTTTACAGCGCCTTAAACTATCTCAACCAAATGGATAAACATATTCTCACCGCCGAAGATCCCGTGGAAATTGAGA
TTGACGGCTTAATTCAAACCCAAGTCAATCAAAGTATTAACCTTGATTTTAGCCAGCTGTTACGCACCTTTCTACGCCAA
GATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAGAGTGCAAAAATGGCATTAAGAGCAAGCCAGACGGGGCA
TTTAGTCCTTTCAACCCTGCATACCAATGACGCACTATCAGCGGTCGAACGTTTGTTACAACTAGGTATTCAAGAGTATG
AAATCCAAAATTCACTACTGTTAGTCATTGCACAACGCCTTGTGCGTAAGCTCTGCAAAAAGTGTCGTGGCACAGGCTGT
GATGAGTGCTATCAAGGCTATAAAGGGCGGATCGGGATTTACCAATGTTTAAGTAGAACTGCAAAAAAATTCGATAAACA
GACCGCTTACTTAGATTTTTCCAGCTTACGAGAGAGTGCTAAACAAAAAATTGAACAACAACTAACCAACGAAATCGAAG
TGGATAGGGTATTAGGCGATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(30-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

97.831

100

0.978
  pilB Haemophilus influenzae 86-028NP

56.957

99.783

0.568
  pilB Haemophilus influenzae Rd KW20

57.582

98.698

0.568
  pilB Vibrio cholerae strain A1552

38.554

100

0.416
  pilB Vibrio campbellii strain DS40M4

39.501

100

0.412
  pilB Vibrio parahaemolyticus RIMD 2210633

39.293

100

0.41
  pilB Acinetobacter baumannii D1279779

36.381

100

0.406
  pilB Acinetobacter baylyi ADP1

36.832

100

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.298

100

0.39
  pilB Legionella pneumophila strain ERS1305867

40.682

95.445

0.388
  pilF Neisseria gonorrhoeae MS11

36.864

100

0.377