Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PUP50_RS28980 Genome accession   NZ_CP118153
Coordinates   6341741..6342958 (+) Length   405 a.a.
NCBI ID   WP_274304663.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain ATCC 17811     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6336741..6347958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUP50_RS28965 (PUP50_28965) - 6337196..6340114 (+) 2919 WP_274304662.1 DUF748 domain-containing protein -
  PUP50_RS28970 (PUP50_28970) - 6340318..6340674 (-) 357 WP_009045666.1 BON domain-containing protein -
  PUP50_RS28975 (PUP50_28975) - 6340961..6341377 (-) 417 WP_007924673.1 pilin -
  PUP50_RS28980 (PUP50_28980) pilC 6341741..6342958 (+) 1218 WP_274304663.1 type II secretion system F family protein Machinery gene
  PUP50_RS28985 (PUP50_28985) pilD 6342961..6343833 (+) 873 WP_274304664.1 prepilin peptidase Machinery gene
  PUP50_RS28990 (PUP50_28990) coaE 6343830..6344453 (+) 624 WP_009045669.1 dephospho-CoA kinase -
  PUP50_RS28995 (PUP50_28995) yacG 6344450..6344653 (+) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  PUP50_RS29000 (PUP50_29000) - 6344658..6344873 (-) 216 WP_053262705.1 hypothetical protein -
  PUP50_RS29005 (PUP50_29005) - 6344939..6345628 (-) 690 WP_009045672.1 energy-coupling factor ABC transporter permease -
  PUP50_RS29010 (PUP50_29010) - 6345625..6346092 (-) 468 WP_274304665.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44507.41 Da        Isoelectric Point: 10.4761

>NTDB_id=790760 PUP50_RS28980 WP_274304663.1 6341741..6342958(+) (pilC) [Pseudomonas chlororaphis strain ATCC 17811]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHTPSLIRAQLRKQGINPARVRKKPSSLLQRTPPIKARDISLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLMGELKQDIAAGSSFTTALRKRPRYFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKTRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPVFTQLVIALSEFVQHWWWLLAGALLLSVFGLR
HARRRSPRFRHWLDVGLLKLPLVAPLMYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKQAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDGMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=790760 PUP50_RS28980 WP_274304663.1 6341741..6342958(+) (pilC) [Pseudomonas chlororaphis strain ATCC 17811]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATACCCCCTCGCTGATCAGGGCCCAGTTGCGCAAGCAAGGCATCAACCCGGCCAGGGTCAGGAAGAAACCCA
GCTCGCTGTTGCAGCGCACGCCCCCGATCAAGGCCCGCGACATCAGCCTGTTCACCCGGCAACTGGCGACCCTGATCAAG
GCCGGCGTGCCACTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGACAACCCGAACATGCGCAAACTGATGGGCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGCTTCACCACCGCGCTGCGCAAGAGGCCGCGGTACTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAACAGGCCGGGGCCCTGGAAACCCTGCTGGAGCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACGCGGATCCGCAAGGCCATGACTTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTCACAGGGAT
CCTGCTGGTCAAGGTGGTGCCGCAATTCCAGTCGATGTTCCAGGGCTTCGGCGCGCAATTGCCGGTGTTCACGCAACTGG
TCATCGCCCTCTCGGAATTCGTGCAGCACTGGTGGTGGCTGCTGGCGGGCGCACTGTTGCTCAGTGTGTTCGGCCTGCGC
CATGCCCGCCGGCGCTCACCACGCTTTCGCCACTGGCTGGACGTGGGCCTGCTGAAACTGCCGTTGGTAGCGCCACTGAT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACTTTCGCCGCCGGAGTGCCGCTGGTGGAGGCCCTGG
GCTCGGTGGCCGGGGCCACCGGCAACCAGGTGTTCAAGCAAGCAGTGAACCGCATCAGGCAGGACGTGGCGACCGGCATG
CAGTTGCATTTTTCCATGCGCGCCTCCGGCATCTTTCCCGGCATGGCCATCCAGATGACCGCCATCGGTGAAGAGTCCGG
GGCCCTGGACGGCATGCTGGAAAAGGTCGCGATCCATTATGAGGAAGAGGTCGATACCCTGGTCGACACCCTGAGCAGTC
TCATGGAACCCGTGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGCACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

64.938

100

0.649
  pilC Acinetobacter baylyi ADP1

56.127

100

0.565
  pilC Acinetobacter baumannii D1279779

55.147

100

0.556
  pilC Legionella pneumophila strain ERS1305867

52.273

97.778

0.511
  pilG Neisseria gonorrhoeae MS11

41.791

99.259

0.415
  pilG Neisseria meningitidis 44/76-A

41.045

99.259

0.407
  pilC Vibrio cholerae strain A1552

42.092

96.79

0.407
  pilC Vibrio campbellii strain DS40M4

38.806

99.259

0.385