Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PUP49_RS28355 Genome accession   NZ_CP118150
Coordinates   6290841..6292058 (+) Length   405 a.a.
NCBI ID   WP_053280960.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain DSM 21509     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6285841..6297058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUP49_RS28340 (PUP49_28340) - 6286284..6289211 (+) 2928 WP_081001595.1 DUF748 domain-containing protein -
  PUP49_RS28345 (PUP49_28345) - 6289413..6289769 (-) 357 WP_053280958.1 BON domain-containing protein -
  PUP49_RS28350 (PUP49_28350) - 6290059..6290475 (-) 417 WP_053280959.1 pilin -
  PUP49_RS28355 (PUP49_28355) pilC 6290841..6292058 (+) 1218 WP_053280960.1 type II secretion system F family protein Machinery gene
  PUP49_RS28360 (PUP49_28360) pilD 6292061..6292933 (+) 873 WP_025807493.1 prepilin peptidase Machinery gene
  PUP49_RS28365 (PUP49_28365) coaE 6292930..6293553 (+) 624 WP_053280961.1 dephospho-CoA kinase -
  PUP49_RS28370 (PUP49_28370) yacG 6293550..6293753 (+) 204 WP_025807495.1 DNA gyrase inhibitor YacG -
  PUP49_RS28375 (PUP49_28375) - 6293758..6293973 (-) 216 WP_053280962.1 hypothetical protein -
  PUP49_RS28380 (PUP49_28380) - 6294039..6294728 (-) 690 WP_025807497.1 energy-coupling factor ABC transporter permease -
  PUP49_RS28385 (PUP49_28385) - 6294725..6295192 (-) 468 WP_053280963.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44470.37 Da        Isoelectric Point: 10.4767

>NTDB_id=790682 PUP49_RS28355 WP_053280960.1 6290841..6292058(+) (pilC) [Pseudomonas chlororaphis strain DSM 21509]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHTPSLIRAQLRKQGISPARVRKKSGSLFQRTPPIKARDISLFTRQLATLLK
AGVPLLQSFNIIAEGFDNPNMRKLMAELKQDIAAGSSFTTALRKRPRYFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKMRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPVFTQLVIALSEFVQRWWWLLAGGLVLSVFGLR
HAHRHSPRFRQWLDVGLLKLPLVAPLMYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKHAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDGMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=790682 PUP49_RS28355 WP_053280960.1 6290841..6292058(+) (pilC) [Pseudomonas chlororaphis strain DSM 21509]
ATGGCGGTCAAGGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATACCCCCTCGCTGATCAGGGCCCAATTGCGCAAGCAAGGCATCAGCCCGGCCAGGGTCAGGAAGAAATCCG
GCTCGCTGTTCCAGCGCACGCCCCCGATCAAGGCCCGCGACATCAGCCTGTTCACCCGGCAGCTGGCGACCCTGCTCAAG
GCCGGCGTACCGCTGCTGCAATCGTTCAACATCATTGCCGAGGGCTTCGACAACCCGAACATGCGCAAACTGATGGCCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGCTTCACCACAGCGTTGCGCAAGAGGCCGCGGTATTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAACAGGCCGGGGCCCTGGAAACCCTGCTGGAGCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGATGCGAATCCGCAAGGCCATGACCTATCCCCTGGCGGTGATCTGTGTGGCCCTGGTGGTGACGGGGAT
CCTGCTGGTCAAGGTGGTGCCGCAATTTCAGTCGATGTTCCAAGGCTTCGGCGCGCAGCTGCCAGTGTTCACCCAACTGG
TCATTGCCCTCTCGGAATTCGTGCAGCGCTGGTGGTGGCTGCTGGCGGGTGGTCTGGTGCTCAGCGTGTTCGGCCTGCGC
CATGCCCACCGGCACTCACCGCGCTTTCGCCAGTGGCTGGACGTGGGCCTGCTGAAACTGCCGTTGGTAGCGCCGCTGAT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACCTTCGCCGCCGGGGTACCGCTGGTGGAGGCCCTGG
GCTCGGTGGCCGGGGCCACCGGCAATCAGGTGTTCAAGCATGCGGTGAACCGCATCAGGCAGGACGTGGCGACCGGCATG
CAGTTGCATTTTTCCATGCGTGCATCCGGTATCTTTCCCGGCATGGCCATCCAGATGACCGCCATCGGCGAAGAGTCCGG
GGCCCTGGACGGCATGCTGGAAAAGGTCGCGATCCACTATGAGGAAGAGGTCGATACCCTGGTCGACACCCTGAGCAGTC
TCATGGAACCCGTGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGTACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

65.432

100

0.654
  pilC Acinetobacter baylyi ADP1

55.637

100

0.56
  pilC Acinetobacter baumannii D1279779

54.412

100

0.548
  pilC Legionella pneumophila strain ERS1305867

52.273

97.778

0.511
  pilG Neisseria gonorrhoeae MS11

41.045

99.259

0.407
  pilC Vibrio cholerae strain A1552

42.092

96.79

0.407
  pilG Neisseria meningitidis 44/76-A

40.299

99.259

0.4
  pilC Vibrio campbellii strain DS40M4

39.303

99.259

0.39