Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PUP66_RS26605 Genome accession   NZ_CP118146
Coordinates   5909588..5910805 (+) Length   405 a.a.
NCBI ID   WP_114761470.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain ATCC 174152     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5904588..5915805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUP66_RS26590 (PUP66_26590) - 5905044..5907962 (+) 2919 WP_114761469.1 DUF748 domain-containing protein -
  PUP66_RS26595 (PUP66_26595) - 5908165..5908521 (-) 357 WP_009045666.1 BON domain-containing protein -
  PUP66_RS26600 (PUP66_26600) - 5908807..5909223 (-) 417 WP_007924673.1 pilin -
  PUP66_RS26605 (PUP66_26605) pilC 5909588..5910805 (+) 1218 WP_114761470.1 type II secretion system F family protein Machinery gene
  PUP66_RS26610 (PUP66_26610) pilD 5910808..5911680 (+) 873 WP_114761471.1 prepilin peptidase Machinery gene
  PUP66_RS26615 (PUP66_26615) coaE 5911677..5912300 (+) 624 WP_009045669.1 dephospho-CoA kinase -
  PUP66_RS26620 (PUP66_26620) yacG 5912297..5912500 (+) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  PUP66_RS26625 (PUP66_26625) - 5912505..5912720 (-) 216 WP_007924682.1 hypothetical protein -
  PUP66_RS26630 (PUP66_26630) - 5912786..5913475 (-) 690 WP_114761472.1 energy-coupling factor ABC transporter permease -
  PUP66_RS26635 (PUP66_26635) - 5913472..5913939 (-) 468 WP_053262707.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44532.48 Da        Isoelectric Point: 10.5902

>NTDB_id=790582 PUP66_RS26605 WP_114761470.1 5909588..5910805(+) (pilC) [Pseudomonas chlororaphis strain ATCC 174152]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHTPSLIRAQLRKQGINPARVRKKPSSLLQRTPPIKARDISLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLMGELKQDIAAGSSFTSALRKRPRHFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKTRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPMFTQLVIALSEFVQRWWWLLAGALLLSVFGLR
HARRRSPRFRHWLDVGLLKLPLIAPLMYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKQAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDGMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=790582 PUP66_RS26605 WP_114761470.1 5909588..5910805(+) (pilC) [Pseudomonas chlororaphis strain ATCC 174152]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATACCCCCTCGCTGATCAGGGCCCAGTTGCGCAAGCAAGGCATCAACCCGGCCAGGGTCAGGAAGAAACCCA
GCTCGCTGTTGCAGCGCACGCCCCCGATCAAGGCCCGCGACATCAGCCTGTTCACCCGGCAACTGGCGACCTTGATCAAG
GCCGGCGTACCACTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGACAACCCGAACATGCGCAAACTGATGGGCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGCTTCACCAGCGCGCTGCGCAAGAGGCCCCGGCACTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAACAGGCCGGGGCCCTGGAAACCCTGCTGGAGCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACGCGGATCCGCAAGGCCATGACCTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTGACGGGGAT
CCTGCTGGTCAAGGTGGTGCCGCAGTTCCAGTCGATGTTCCAGGGCTTCGGCGCGCAATTGCCCATGTTCACGCAACTGG
TCATTGCCCTCTCGGAATTCGTGCAGCGCTGGTGGTGGCTGCTGGCGGGCGCGCTGTTGCTCAGTGTGTTCGGCCTGCGC
CATGCCCGCCGGCGCTCACCGCGCTTTCGCCACTGGCTGGACGTGGGCCTGCTGAAATTGCCGTTGATAGCGCCATTGAT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTATCCACCACTTTCGCCGCCGGAGTGCCGCTGGTGGAGGCCCTGG
GCTCGGTGGCCGGGGCCACCGGCAACCAGGTGTTCAAGCAAGCGGTGAACCGCATCAGGCAGGACGTGGCGACCGGCATG
CAGTTGCATTTTTCCATGCGTGCATCCGGCATCTTTCCCGGCATGGCCATCCAGATGACCGCCATCGGCGAAGAGTCCGG
GGCCCTGGACGGCATGCTGGAAAAGGTCGCGATCCATTATGAGGAAGAGGTCGATACCCTGGTCGACACCCTGAGCAGTC
TCATGGAACCCGTGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGCACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

64.691

100

0.647
  pilC Acinetobacter baylyi ADP1

55.637

100

0.56
  pilC Acinetobacter baumannii D1279779

55.637

100

0.56
  pilC Legionella pneumophila strain ERS1305867

52.273

97.778

0.511
  pilC Vibrio cholerae strain A1552

42.602

96.79

0.412
  pilG Neisseria gonorrhoeae MS11

41.542

99.259

0.412
  pilG Neisseria meningitidis 44/76-A

40.796

99.259

0.405
  pilC Vibrio campbellii strain DS40M4

39.055

99.259

0.388