Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PUP62_RS27760 Genome accession   NZ_CP118143
Coordinates   6147275..6148492 (+) Length   405 a.a.
NCBI ID   WP_123573554.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain ATCC 174182     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6142275..6153492
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUP62_RS27745 (PUP62_27745) - 6142769..6145687 (+) 2919 WP_274330484.1 DUF748 domain-containing protein -
  PUP62_RS27750 (PUP62_27750) - 6145853..6146209 (-) 357 WP_009045666.1 BON domain-containing protein -
  PUP62_RS27755 (PUP62_27755) - 6146495..6146911 (-) 417 WP_007924673.1 pilin -
  PUP62_RS27760 (PUP62_27760) pilC 6147275..6148492 (+) 1218 WP_123573554.1 type II secretion system F family protein Machinery gene
  PUP62_RS27765 (PUP62_27765) pilD 6148495..6149367 (+) 873 WP_274330488.1 prepilin peptidase Machinery gene
  PUP62_RS27770 (PUP62_27770) coaE 6149364..6149987 (+) 624 WP_009045669.1 dephospho-CoA kinase -
  PUP62_RS27775 (PUP62_27775) yacG 6149984..6150187 (+) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  PUP62_RS27780 (PUP62_27780) - 6150192..6150407 (-) 216 WP_009045671.1 hypothetical protein -
  PUP62_RS27785 (PUP62_27785) - 6150473..6151162 (-) 690 WP_009045672.1 energy-coupling factor ABC transporter permease -
  PUP62_RS27790 (PUP62_27790) - 6151159..6151626 (-) 468 WP_123412471.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44531.47 Da        Isoelectric Point: 10.5241

>NTDB_id=790504 PUP62_RS27760 WP_123573554.1 6147275..6148492(+) (pilC) [Pseudomonas chlororaphis strain ATCC 174182]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHTPSLIRAQLRKQGINPARVRKKPSSLLQRTPPIKARDISLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLMGELKQDIAAGSSFTSALRKRPRHFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKTRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPMFTQLVIALSEFVQHWWWLLAGALLLSMFGLR
HARRRSPRFRHWLDVGLLKLPLVAPLMYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKQAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDGMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=790504 PUP62_RS27760 WP_123573554.1 6147275..6148492(+) (pilC) [Pseudomonas chlororaphis strain ATCC 174182]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATACCCCCTCACTGATCAGGGCCCAGTTGCGCAAGCAAGGCATCAACCCGGCCAGGGTCAGGAAGAAACCCA
GCTCGCTGTTGCAGCGCACGCCCCCGATCAAGGCCCGCGACATCAGCCTGTTCACCCGGCAACTGGCGACCTTGATCAAG
GCCGGCGTACCACTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGACAACCCGAACATGCGCAAACTGATGGGCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGCTTCACCAGCGCGCTGCGCAAGAGGCCCCGGCACTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAACAGGCCGGGGCCCTGGAAACCCTGCTGGAGCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACGCGGATCCGCAAGGCCATGACCTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTGACGGGGAT
CCTGCTGGTCAAGGTGGTGCCGCAGTTCCAGTCGATGTTCCAGGGCTTCGGCGCGCAATTGCCCATGTTCACGCAACTGG
TCATCGCCCTCTCGGAATTCGTGCAGCACTGGTGGTGGCTGCTGGCAGGCGCGCTGTTGCTCAGTATGTTTGGCCTGCGC
CATGCCCGCCGGCGCTCACCGCGCTTTCGCCACTGGCTGGACGTGGGCCTGCTGAAACTGCCGTTGGTAGCGCCACTGAT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACCTTCGCCGCCGGAGTGCCGCTGGTGGAGGCCCTGG
GCTCGGTGGCCGGGGCCACCGGCAACCAGGTGTTCAAGCAAGCGGTGAACCGCATCAGGCAGGACGTGGCGACCGGCATG
CAGTTGCATTTTTCCATGCGTGCATCCGGCATCTTTCCCGGCATGGCCATCCAGATGACCGCCATCGGCGAAGAGTCCGG
GGCCCTGGACGGCATGCTGGAAAAGGTCGCGATCCATTATGAGGAAGAGGTCGATACCCTGGTCGACACCCTGAGCAGTC
TCATGGAACCCGTGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGCACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

64.938

100

0.649
  pilC Acinetobacter baylyi ADP1

55.882

100

0.563
  pilC Acinetobacter baumannii D1279779

55.637

100

0.56
  pilC Legionella pneumophila strain ERS1305867

51.768

97.778

0.506
  pilG Neisseria gonorrhoeae MS11

41.542

99.259

0.412
  pilC Vibrio cholerae strain A1552

42.347

96.79

0.41
  pilG Neisseria meningitidis 44/76-A

40.796

99.259

0.405
  pilC Vibrio campbellii strain DS40M4

39.055

99.259

0.388