Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PUP68_RS28995 Genome accession   NZ_CP118139
Coordinates   6448581..6449798 (+) Length   405 a.a.
NCBI ID   WP_274321582.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain DSM 295781     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6443581..6454798
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUP68_RS28980 (PUP68_28980) - 6444036..6446951 (+) 2916 WP_274321584.1 DUF748 domain-containing protein -
  PUP68_RS28985 (PUP68_28985) - 6447153..6447509 (-) 357 WP_053280958.1 BON domain-containing protein -
  PUP68_RS28990 (PUP68_28990) - 6447799..6448215 (-) 417 WP_274321583.1 pilin -
  PUP68_RS28995 (PUP68_28995) pilC 6448581..6449798 (+) 1218 WP_274321582.1 type II secretion system F family protein Machinery gene
  PUP68_RS29000 (PUP68_29000) pilD 6449801..6450673 (+) 873 WP_025807493.1 prepilin peptidase Machinery gene
  PUP68_RS29005 (PUP68_29005) coaE 6450670..6451293 (+) 624 WP_274321581.1 dephospho-CoA kinase -
  PUP68_RS29010 (PUP68_29010) yacG 6451290..6451493 (+) 204 WP_025807495.1 DNA gyrase inhibitor YacG -
  PUP68_RS29015 (PUP68_29015) - 6451498..6451713 (-) 216 WP_053280962.1 hypothetical protein -
  PUP68_RS29020 (PUP68_29020) - 6451779..6452468 (-) 690 WP_025807497.1 energy-coupling factor ABC transporter permease -
  PUP68_RS29025 (PUP68_29025) - 6452465..6452932 (-) 468 WP_274321580.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44471.35 Da        Isoelectric Point: 10.4090

>NTDB_id=790399 PUP68_RS28995 WP_274321582.1 6448581..6449798(+) (pilC) [Pseudomonas chlororaphis strain DSM 295781]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHTPSLIRAQLRKQGISPARVRKKSGSLFQRTPPIKARDISLFTRQLATLLK
AGVPLLQSFDIIAEGFDNPNMRKLMAELKQDIAAGSSFTTALRKRPRYFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKMRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPVFTQLVIALSEFVQRWWWLLAGGLVLSVFGLR
HAHRHSPRFRQWLDVGLLKLPLVAPLMYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKHAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDGMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=790399 PUP68_RS28995 WP_274321582.1 6448581..6449798(+) (pilC) [Pseudomonas chlororaphis strain DSM 295781]
ATGGCGGTCAAGGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATACCCCCTCGCTGATCAGGGCCCAATTGCGCAAGCAAGGCATCAGCCCGGCCAGGGTCAGGAAGAAATCCG
GCTCGCTGTTCCAGCGCACGCCCCCGATCAAGGCCCGCGACATCAGCCTGTTCACCCGGCAGCTGGCGACCCTGCTCAAG
GCCGGCGTACCGCTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGACAACCCGAACATGCGCAAACTGATGGCCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGCTTCACCACAGCGTTGCGCAAGAGGCCGCGGTATTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAACAGGCCGGGGCCCTGGAAACCCTGCTGGAGCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGATGCGAATCCGCAAGGCCATGACCTATCCCCTGGCGGTGATCTGTGTGGCCCTGGTGGTCACGGGGAT
CCTGCTGGTCAAGGTGGTGCCGCAATTCCAGTCGATGTTCCAAGGCTTCGGCGCGCAACTGCCGGTGTTCACCCAACTGG
TCATTGCCCTCTCGGAATTCGTGCAGCGCTGGTGGTGGCTGCTGGCGGGTGGTCTGGTGCTCAGCGTGTTCGGCCTGCGC
CATGCCCACCGGCACTCACCGCGCTTTCGCCAGTGGCTGGACGTGGGCCTGCTGAAACTGCCGTTGGTAGCGCCGCTGAT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACCTTCGCCGCCGGGGTACCGCTGGTGGAGGCCCTGG
GCTCGGTGGCCGGGGCCACCGGCAATCAGGTGTTCAAGCATGCGGTGAACCGCATCAGGCAGGACGTGGCGACCGGCATG
CAGTTGCATTTTTCCATGCGTGCATCCGGTATCTTTCCCGGCATGGCCATCCAGATGACCGCCATCGGCGAAGAGTCCGG
GGCCCTGGACGGCATGCTGGAAAAGGTCGCGATCCACTATGAGGAAGAGGTCGATACCCTGGTCGACACCCTGAGCAGTC
TCATGGAACCCGTGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGTACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

65.679

100

0.657
  pilC Acinetobacter baylyi ADP1

55.637

100

0.56
  pilC Acinetobacter baumannii D1279779

54.412

100

0.548
  pilC Legionella pneumophila strain ERS1305867

52.525

97.778

0.514
  pilG Neisseria gonorrhoeae MS11

41.045

99.259

0.407
  pilC Vibrio cholerae strain A1552

42.092

96.79

0.407
  pilG Neisseria meningitidis 44/76-A

40.299

99.259

0.4
  pilC Vibrio campbellii strain DS40M4

39.303

99.259

0.39