Detailed information NTG0790400

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PUP68_RS29000
• Genome accession: NZ_CP118139
• Coordinates: 6449801..6450673 (+)
• Length: 290 a.a.
• NCBI ID: WP_025807493.1
• Uniprot ID: -
• Organism: Pseudomonas chlororaphis strain DSM 295781
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6444801..6455673

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PUP68_RS28985 (PUP68_28985)	-	6447153..6447509 (-)	357	WP_053280958.1	BON domain-containing protein	-
PUP68_RS28990 (PUP68_28990)	-	6447799..6448215 (-)	417	WP_274321583.1	pilin	-
PUP68_RS28995 (PUP68_28995)	pilC	6448581..6449798 (+)	1218	WP_274321582.1	type II secretion system F family protein	Machinery gene
PUP68_RS29000 (PUP68_29000)	pilD	6449801..6450673 (+)	873	WP_025807493.1	prepilin peptidase	Machinery gene
PUP68_RS29005 (PUP68_29005)	coaE	6450670..6451293 (+)	624	WP_274321581.1	dephospho-CoA kinase	-
PUP68_RS29010 (PUP68_29010)	yacG	6451290..6451493 (+)	204	WP_025807495.1	DNA gyrase inhibitor YacG	-
PUP68_RS29015 (PUP68_29015)	-	6451498..6451713 (-)	216	WP_053280962.1	hypothetical protein	-
PUP68_RS29020 (PUP68_29020)	-	6451779..6452468 (-)	690	WP_025807497.1	energy-coupling factor ABC transporter permease	-
PUP68_RS29025 (PUP68_29025)	-	6452465..6452932 (-)	468	WP_274321580.1	FAD/FMN-containing dehydrogenase	-
PUP68_RS29030 (PUP68_29030)	-	6453388..6454824 (+)	1437	WP_053280964.1	undecaprenyl-phosphate glucose phosphotransferase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32014.10 Da
• Isoelectric Point: 8.2548

>NTDB_id=790400 PUP68_RS29000 WP_025807493.1 6449801..6450673(+) (pilD) [Pseudomonas chlororaphis strain DSM 295781]
MSLTEVLASYPLVFVASVLVLGLIVGSFLNVLIWRLPKMLERDWHAQARDILGLPVEPAGPTYNLLLPHSQCPHCAHPIR
PWENIPVFSYLLLRGRCSACKTPIGRRYPLVELTCGLASAFVAWHFGFGWQAGLMLFLSWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIVNGFDLFVSLHDALWGAVAGYLCLWSVYWSFKLLTGKEGMGHGDFKLLAMFGAWGGWQILPMTLLLASLTG
ALAGVAMLRRNRAQMSTPIPFGPFLAIAGWIALLWGGQITGSYLQFVGFR

Nucleotide

• Length: 873 bp

>NTDB_id=790400 PUP68_RS29000 WP_025807493.1 6449801..6450673(+) (pilD) [Pseudomonas chlororaphis strain DSM 295781]
ATGTCATTGACTGAGGTCCTGGCCAGCTACCCGCTGGTCTTCGTAGCATCGGTGCTGGTCCTGGGCCTGATTGTCGGCAG
CTTCCTCAACGTGCTGATCTGGCGGCTGCCAAAGATGCTCGAGCGCGACTGGCATGCCCAGGCCCGGGACATCCTGGGGC
TGCCCGTGGAACCTGCCGGGCCGACCTACAACCTGTTGCTCCCACACTCCCAGTGCCCTCACTGCGCGCACCCGATCCGC
CCCTGGGAAAACATCCCAGTGTTCAGCTACCTGCTGCTGCGCGGACGCTGCTCGGCGTGCAAGACCCCGATCGGCCGGCG
CTACCCGCTGGTGGAGCTGACCTGCGGCCTGGCCTCGGCGTTCGTGGCCTGGCATTTCGGCTTTGGCTGGCAGGCCGGCT
TGATGCTGTTCCTGAGCTGGGGCCTGTTGGCGATGAGCCTGATCGATGCCGATCATCAACTGTTGCCCGACGTGCTGGTG
CTGCCCCTGTTGTGGCTGGGCCTGATCGTCAATGGTTTCGACCTGTTCGTCAGCCTCCACGATGCGCTGTGGGGCGCCGT
GGCCGGCTACCTGTGCCTGTGGTCGGTGTACTGGTCGTTCAAGCTGCTGACCGGCAAGGAAGGCATGGGGCACGGCGATT
TCAAGCTGTTGGCCATGTTCGGCGCCTGGGGCGGCTGGCAGATTCTGCCGATGACCCTGCTGCTGGCCTCACTGACGGGA
GCTCTAGCCGGGGTTGCCATGCTGCGCCGCAACCGCGCGCAAATGTCCACGCCTATCCCTTTTGGTCCCTTTCTGGCCAT
TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGGCTCCTATTTGCAGTTTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.004	97.586	0.517
pilD	Vibrio campbellii strain DS40M4	51.064	97.241	0.497
pilD	Acinetobacter baumannii D1279779	49.815	93.448	0.466
pilD	Acinetobacter nosocomialis M2	49.077	93.448	0.459
pilD	Neisseria gonorrhoeae MS11	48.162	93.793	0.452