Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA/celA/cilE   Type   Machinery gene
Locus tag   PUW70_RS05255 Genome accession   NZ_CP118029
Coordinates   1034570..1035274 (-) Length   234 a.a.
NCBI ID   WP_024051601.1    Uniprot ID   A0A412PQ70
Organism   Streptococcus anginosus strain VSI68     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1029570..1040274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW70_RS05240 (PUW70_05240) - 1030100..1031086 (-) 987 WP_024051597.1 Gfo/Idh/MocA family oxidoreductase -
  PUW70_RS05245 (PUW70_05245) - 1031716..1032276 (-) 561 WP_224783657.1 DUF805 domain-containing protein -
  PUW70_RS05250 (PUW70_05250) comEC/celB 1032358..1034586 (-) 2229 WP_024051600.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  PUW70_RS05255 (PUW70_05255) comEA/celA/cilE 1034570..1035274 (-) 705 WP_024051601.1 helix-hairpin-helix domain-containing protein Machinery gene
  PUW70_RS05260 (PUW70_05260) smpB 1035457..1035924 (-) 468 WP_024051602.1 SsrA-binding protein SmpB -
  PUW70_RS05265 (PUW70_05265) rnr 1035887..1038226 (-) 2340 WP_101750372.1 ribonuclease R -
  PUW70_RS05270 (PUW70_05270) secG 1038318..1038551 (-) 234 WP_003024744.1 preprotein translocase subunit SecG -
  PUW70_RS05275 (PUW70_05275) rpmG 1038591..1038740 (-) 150 WP_003024746.1 50S ribosomal protein L33 -
  PUW70_RS05280 (PUW70_05280) - 1038724..1039929 (-) 1206 WP_180757762.1 multidrug efflux MFS transporter -

Sequence

Protein


Download         Length: 234 a.a.        Molecular weight: 24966.91 Da        Isoelectric Point: 4.7586

>NTDB_id=789253 PUW70_RS05255 WP_024051601.1 1034570..1035274(-) (comEA/celA/cilE) [Streptococcus anginosus strain VSI68]
MLETIIEKMKEYKILIGLSLIGLIIAGFFMINVQSSRQSNVAELAQETVTSSGAESEEISTGTKKNSQKEKAEPQTSSSE
ESEFLTVDVKGAVKNPGIYQLKKTSRINDAIQKAGGLTTDADSKSINLAQKLTDEAVVYVATMGENAASVSSNTGQSSTS
GTSEVASQKGNKVNLNTADLSELQTISGIGQKRAQDILDYREANGKFNSVDDLKNVSGVGAKTLEKLKEYVTVD

Nucleotide


Download         Length: 705 bp        

>NTDB_id=789253 PUW70_RS05255 WP_024051601.1 1034570..1035274(-) (comEA/celA/cilE) [Streptococcus anginosus strain VSI68]
ATGTTAGAGACAATCATTGAAAAGATGAAAGAGTATAAAATTTTGATTGGTTTAAGTTTGATTGGTTTGATAATAGCAGG
ATTTTTTATGATAAATGTTCAATCTAGTAGACAATCAAATGTAGCAGAGCTCGCACAGGAAACAGTTACGAGTTCGGGGG
CAGAATCAGAAGAAATTTCAACTGGAACGAAGAAAAACTCACAGAAAGAAAAAGCAGAGCCTCAAACGAGTTCAAGTGAA
GAATCAGAATTTTTAACCGTAGATGTCAAGGGTGCAGTCAAAAATCCAGGTATTTATCAACTGAAAAAGACTAGCCGTAT
CAATGATGCGATTCAAAAAGCAGGTGGTTTAACGACAGACGCTGACAGCAAGTCTATCAATTTGGCGCAGAAACTAACGG
ATGAAGCTGTTGTTTATGTGGCAACTATGGGTGAGAATGCGGCGAGTGTTTCAAGTAATACAGGACAATCTTCAACGTCA
GGAACTAGTGAAGTCGCATCGCAAAAAGGAAATAAGGTCAATCTAAATACAGCTGATTTATCTGAGCTGCAGACCATTTC
TGGTATTGGTCAAAAACGTGCGCAAGATATTTTAGATTATCGTGAGGCAAACGGGAAATTTAATTCCGTTGATGACTTGA
AAAATGTATCAGGAGTAGGCGCTAAAACACTAGAAAAATTGAAAGAATATGTCACAGTGGATTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(87-140)

Helix-hairpin-helix motif (HHH_3)

(169-232)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A412PQ70

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA/celA/cilE Streptococcus pneumoniae D39

53.219

99.573

0.53
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

53.219

99.573

0.53
  comEA/celA/cilE Streptococcus pneumoniae R6

53.219

99.573

0.53
  comEA/celA/cilE Streptococcus pneumoniae Rx1

53.219

99.573

0.53
  comEA/celA/cilE Streptococcus mitis NCTC 12261

51.502

99.573

0.513
  comEA/celA/cilE Streptococcus mitis SK321

50.215

99.573

0.5
  comEA Streptococcus thermophilus LMD-9

44.255

100

0.444
  comEA Lactococcus lactis subsp. cremoris KW2

42.06

99.573

0.419