Detailed information NTG0789252

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: PUW70_RS05250
• Genome accession: NZ_CP118029
• Coordinates: 1032358..1034586 (-)
• Length: 742 a.a.
• NCBI ID: WP_024051600.1
• Uniprot ID: A0A412PQ79
• Organism: Streptococcus anginosus strain VSI68
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1027358..1039586

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PUW70_RS05225 (PUW70_05225)	-	1027626..1028903 (-)	1278	WP_070815179.1	dihydroorotase	-
PUW70_RS05230 (PUW70_05230)	-	1028916..1029386 (-)	471	WP_024051595.1	8-oxo-dGTP diphosphatase	-
PUW70_RS05235 (PUW70_05235)	-	1029423..1030076 (-)	654	WP_024051596.1	uracil-DNA glycosylase	-
PUW70_RS05240 (PUW70_05240)	-	1030100..1031086 (-)	987	WP_024051597.1	Gfo/Idh/MocA family oxidoreductase	-
PUW70_RS05245 (PUW70_05245)	-	1031716..1032276 (-)	561	WP_224783657.1	DUF805 domain-containing protein	-
PUW70_RS05250 (PUW70_05250)	comEC/celB	1032358..1034586 (-)	2229	WP_024051600.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
PUW70_RS05255 (PUW70_05255)	comEA/celA/cilE	1034570..1035274 (-)	705	WP_024051601.1	helix-hairpin-helix domain-containing protein	Machinery gene
PUW70_RS05260 (PUW70_05260)	smpB	1035457..1035924 (-)	468	WP_024051602.1	SsrA-binding protein SmpB	-
PUW70_RS05265 (PUW70_05265)	rnr	1035887..1038226 (-)	2340	WP_101750372.1	ribonuclease R	-
PUW70_RS05270 (PUW70_05270)	secG	1038318..1038551 (-)	234	WP_003024744.1	preprotein translocase subunit SecG	-
PUW70_RS05275 (PUW70_05275)	rpmG	1038591..1038740 (-)	150	WP_003024746.1	50S ribosomal protein L33	-

Sequence

Protein

• Length: 742 a.a.
• Molecular weight: 85068.91 Da
• Isoelectric Point: 9.9918

>NTDB_id=789252 PUW70_RS05250 WP_024051600.1 1032358..1034586(-) (comEC/celB) [Streptococcus anginosus strain VSI68]
MSQWIKKFPIKPIYIAFLLVWLYFAIYPSSWLGWLGFIFLVIRLFRFYSPKECFMTFMILSCFASFFFIRREIAEQQTKA
EPSPIKQVAVLPDTIKVNGDSLSFRGKANRQTYQVYHKLKSKEEQLAFQNLSSLVALTVEGDFEIPEKKRNFAGFDYQSY
LKTQGIYRILKVDTILSSQDRISLHPLEWLSSWRRKALVFIKNHFPNPMSNYMTGLLFGALDTDFDEMSNLYSSLGIIHL
FALSGMQVGFFMEGFRKLLLRLGFTQEMVRKCQYPFSFFYAGMTGFSVSVVRSLVQKLLSQHGITKLDNFALTMMILSLI
MPSFLLSAGGVLSCVYAFVISVIDFEGLASWRKVVVESSVISLGILPILIFYFGEFQPWSILLTFVFSLIFDAMMLPGLT
LIFLFSPLIKLTQVNFLFEGLENSIRWIASVFGKPIVFGQPSPLLLIVMLLVLAILYDVRKNKKWVIFLSLFLSLLFFIN
KFPLQNEITMVDVGQGDSIFLRDWKGKNVLIDVGGREEIRTKEAWQKRAISSNAEKTLIPYLKSRGVDTIDTLVLTNPNP
DYAGDVLGVAKKFAIKKIYISRSSLSNADFLEKLRKLNTFIHVVKQGDKLPIFDHHLQVLSGVSKNDHSIVLYGQFFRTR
FLFASDLKEEGEAKLMQHYPKLKTDVLKVGQHEAKDSSSSKFLQQIEPTVALISVEKNNQSKQPSQDTIERFAQLPAKVY
RTDEQGAVKFSGWTNWRLEMVK

Nucleotide

• Length: 2229 bp

>NTDB_id=789252 PUW70_RS05250 WP_024051600.1 1032358..1034586(-) (comEC/celB) [Streptococcus anginosus strain VSI68]
ATGTCACAGTGGATTAAAAAATTTCCGATTAAGCCGATTTACATTGCTTTCTTACTTGTCTGGTTATATTTTGCAATCTA
TCCAAGTAGCTGGTTAGGCTGGTTGGGTTTTATCTTTCTGGTAATTCGTCTTTTTCGCTTTTATTCACCGAAAGAATGTT
TCATGACCTTCATGATCCTCTCTTGTTTTGCTAGTTTTTTCTTTATTCGTAGGGAAATAGCAGAGCAGCAAACAAAAGCA
GAGCCTTCTCCCATAAAACAAGTGGCAGTTCTACCTGACACGATTAAGGTAAATGGAGATTCGCTTTCTTTTCGTGGTAA
AGCTAATAGGCAGACTTATCAAGTTTACCACAAATTGAAATCAAAGGAAGAGCAGTTGGCTTTTCAAAATCTCTCTAGTT
TGGTTGCGTTGACCGTTGAAGGAGATTTTGAAATTCCTGAGAAGAAGCGTAATTTTGCTGGTTTTGATTACCAATCTTAT
TTAAAAACGCAAGGGATTTATCGAATTTTAAAAGTAGATACCATTTTATCGAGCCAAGATAGAATCAGCTTGCACCCTTT
GGAGTGGCTCTCTAGCTGGCGAAGAAAGGCACTGGTATTTATCAAGAACCATTTTCCAAATCCGATGAGTAATTATATGA
CGGGGCTCTTATTTGGCGCCTTGGATACAGACTTTGACGAAATGAGCAATCTTTATTCTAGCTTGGGAATTATTCATTTA
TTTGCGCTGTCTGGCATGCAAGTTGGCTTTTTTATGGAGGGATTTCGCAAGTTACTGTTAAGGCTGGGGTTCACACAAGA
AATGGTTCGTAAATGCCAATATCCATTTTCTTTCTTTTATGCGGGAATGACTGGATTTTCAGTATCCGTTGTACGGAGCT
TAGTTCAGAAATTATTATCGCAACATGGTATCACTAAGTTAGATAATTTTGCTTTAACGATGATGATATTGTCCTTGATT
ATGCCGTCCTTTCTTTTGTCGGCAGGAGGAGTTCTCTCCTGTGTCTATGCTTTTGTCATTAGTGTGATAGATTTTGAAGG
TCTGGCTTCTTGGCGAAAAGTTGTTGTAGAGAGTAGCGTTATTTCACTTGGTATTTTACCGATTCTAATCTTTTACTTTG
GCGAATTTCAACCTTGGTCTATTTTGTTGACATTTGTTTTTTCACTGATTTTTGATGCAATGATGTTGCCAGGCTTGACG
TTAATTTTTCTTTTTTCGCCTTTGATAAAGTTGACTCAAGTCAATTTTCTATTTGAAGGTTTAGAAAATAGTATTCGTTG
GATAGCAAGTGTCTTTGGTAAACCAATCGTTTTTGGGCAGCCCAGTCCGCTTTTGCTGATTGTTATGCTGCTTGTACTGG
CTATTTTGTATGATGTTAGAAAAAATAAAAAATGGGTAATATTTCTTAGTCTGTTTCTTTCATTACTCTTTTTCATAAAT
AAATTTCCTTTGCAAAACGAGATCACAATGGTGGATGTCGGGCAAGGAGATAGTATTTTTCTGAGAGACTGGAAAGGAAA
AAATGTATTGATTGATGTTGGCGGACGTGAGGAAATTAGAACAAAAGAAGCTTGGCAAAAACGAGCAATTAGCTCAAATG
CAGAAAAAACTTTGATTCCTTATCTAAAAAGTCGTGGTGTAGATACGATTGATACTTTAGTCTTAACAAATCCTAATCCA
GATTATGCAGGAGATGTATTAGGAGTTGCTAAAAAGTTTGCAATAAAAAAAATTTATATTTCCAGAAGTAGTCTAAGCAA
TGCAGATTTTCTAGAGAAATTAAGGAAACTAAACACATTCATTCATGTTGTAAAACAAGGCGACAAACTTCCTATTTTTG
ATCATCATTTGCAAGTTCTTTCGGGTGTTAGCAAGAACGACCATTCGATCGTTTTATATGGTCAGTTTTTCCGTACAAGA
TTTTTATTTGCAAGCGACTTGAAAGAAGAGGGCGAAGCAAAGTTAATGCAGCATTATCCAAAGTTGAAAACAGATGTTTT
GAAAGTCGGACAGCATGAAGCCAAGGACTCATCAAGTTCAAAATTTTTGCAACAAATAGAACCAACGGTTGCGCTCATTT
CTGTTGAGAAAAATAATCAATCTAAGCAACCTAGTCAAGATACAATAGAGCGATTTGCACAATTGCCTGCTAAAGTCTAT
CGGACAGATGAACAAGGGGCAGTTAAATTTTCAGGGTGGACAAACTGGCGATTAGAGATGGTCAAGTAA

Domains

Predicted by InterproScan.

• Competence protein (Competence) (216-460)

• Domain of unknown function (DUF4131) (DUF4131) (28-175)

• Metallo-beta-lactamase superfamily (Lactamase_B) (492-592)

3D structure

• AlphaFold DB: A0A412PQ79

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus mitis SK321	54.618	100	0.55
comEC/celB	Streptococcus mitis NCTC 12261	53.887	100	0.542
comEC/celB	Streptococcus pneumoniae TIGR4	53.548	100	0.539
comEC/celB	Streptococcus pneumoniae Rx1	52.744	100	0.531
comEC/celB	Streptococcus pneumoniae D39	52.744	100	0.531
comEC/celB	Streptococcus pneumoniae R6	52.744	100	0.531
comEC	Lactococcus lactis subsp. cremoris KW2	47.517	100	0.477