Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PUB92_RS18985 Genome accession   NZ_CP117919
Coordinates   4189820..4191526 (+) Length   568 a.a.
NCBI ID   WP_017766420.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain PSKL.DM     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4184820..4196526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUB92_RS18960 (PUB92_18960) pdhR 4185992..4186756 (-) 765 WP_005305630.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  PUB92_RS18965 (PUB92_18965) ampD 4187032..4187604 (-) 573 WP_019840656.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PUB92_RS18970 (PUB92_18970) - 4187728..4188198 (+) 471 WP_017779055.1 retropepsin-like aspartic protease family protein -
  PUB92_RS18975 (PUB92_18975) nadC 4188203..4189060 (+) 858 WP_274181719.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PUB92_RS18980 (PUB92_18980) - 4189382..4189816 (+) 435 WP_041203005.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PUB92_RS18985 (PUB92_18985) pilB 4189820..4191526 (+) 1707 WP_017766420.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  PUB92_RS18990 (PUB92_18990) pilC 4191642..4192883 (+) 1242 WP_274181720.1 type II secretion system F family protein Machinery gene
  PUB92_RS18995 (PUB92_18995) pilD 4192906..4193778 (+) 873 WP_042007258.1 prepilin peptidase Machinery gene
  PUB92_RS19000 (PUB92_19000) coaE 4193798..4194412 (+) 615 WP_242762039.1 dephospho-CoA kinase -
  PUB92_RS19005 (PUB92_19005) zapD 4194450..4195172 (+) 723 WP_025202564.1 cell division protein ZapD -
  PUB92_RS19010 (PUB92_19010) yacG 4195182..4195376 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  PUB92_RS19015 (PUB92_19015) mutT 4195437..4195850 (-) 414 WP_274181721.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62542.93 Da        Isoelectric Point: 5.4342

>NTDB_id=788357 PUB92_RS18985 WP_017766420.1 4189820..4191526(+) (pilB) [Aeromonas dhakensis strain PSKL.DM]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAISKLMENDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKMSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEILLMSENIAKLIMQGANSMQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=788357 PUB92_RS18985 WP_017766420.1 4189820..4191526(+) (pilB) [Aeromonas dhakensis strain PSKL.DM]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGTCTGGCCGCTTCTTCCCTGCTCAGTGAGTCCGACTCACAGCGCTA
TCTGAGCCAGGCCAAGGCGCAGCGCAAACCTTTTGTGACCTTTCTGATCGAGAACGAGATCCTCGACAGCAAGGCGCTGG
CCGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTACTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCCGA
CCCGACCAATGTGTCGGCACTGGAGGATTTTGGTTTCAGCTTCGGGCTGCACACCGAGGCCCTGCTGGTCGAAGAGAACA
AGCTGACCGCCGCCATCAGCAAGCTGATGGAGAACGATCAGGACGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAGCTGGAGGTGTCTGACGAGGGGTCACGACTCGACGAGAGCGTCAACACGGCGGACGATGACGCCCCCATCGTCAA
ATACATCAACAAGATCATGATGGATGCCATCAAGCGCGGTGCATCCGACCTCCACTTCGAACCCTACGAGACCAAATACC
GTATTCGCTTTCGGATAGACGGGATCCTGCATGAGGTAGCGACGCCACCGGTCAATCTGGCCAACCGCTTCTCTGCCCGC
CTCAAGGTGATGGCGCGCCTGGACATCGCCGAACGGCGGCTGCCCCAGGATGGCCGCATCAAATTGAAGATGTCGCGCAA
CAAGTCGATGGACATGCGGGTCAACACACTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTCGACTCTTCCG
CTGCGCGCCTCAATATCGAACAGCTCGGCTTTGACGATCGGCAGAAGGCACAATATCTGCGCGCGCTCTCGAAGCCTCAG
GGCATGATCCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTCTACACCGGCCTGAACATTCTCAACACCAC
AGAAGTGAATATTTCAACCGCCGAAGACCCGGTGGAGATCAACCTGCCTGGGGTCAATCAGGTACAGGTCAACCCCAAGG
CCGGGCTGACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACGTAGTGATGGTCGGGGAAATACGGGAT
CTTGAAACCGCCGAGATCGCCATCAAGGCGGCACAAACCGGCCACCTGGTGCTCTCCACATTGCACACCAACTCGGCGGC
CGAAACACTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTTACCCTGATCATGGCCCAGC
GACTGGCACGCAAGCTGTGTGACCACTGCAAGGCCCCCGAAGTGGTGCCGGAGGCTGAACTGTTGGAGTTGGGGTTCACC
CAACAGCAGCTGGCAGCAGGCTTGCGGCTGTTCAAGCCGGTTGGTTGCAAGGAGTGTTCGGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCCTGTTGATGTCCGAAAATATTGCAAAATTGATCATGCAGGGAGCCAACTCCATGCAAATTGCGG
CCATTGCCCAGAAGGAGGGCATGCGCACCTTGCGGACCTCCGGTCTTGAGAAGGCCCGGCTGGGGGTGACCAGCCTGGCC
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Acinetobacter baumannii D1279779

54.833

100

0.549
  pilB Legionella pneumophila strain ERS1305867

54.417

99.648

0.542
  pilB Vibrio cholerae strain A1552

53.805

99.472

0.535
  pilB Vibrio campbellii strain DS40M4

51.59

99.648

0.514
  pilB Vibrio parahaemolyticus RIMD 2210633

50.89

98.944

0.504
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.698

97.359

0.377
  pilF Thermus thermophilus HB27

40.53

92.958

0.377