Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PUB83_RS21215 Genome accession   NZ_CP117918
Coordinates   4650941..4652656 (-) Length   571 a.a.
NCBI ID   WP_259231738.1    Uniprot ID   -
Organism   Aeromonas hydrophila subsp. hydrophila strain PSKL.DP     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4645941..4657656
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUB83_RS21185 (PUB83_18655) mutT 4646617..4647030 (+) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -
  PUB83_RS21190 (PUB83_18660) yacG 4647091..4647285 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  PUB83_RS21195 (PUB83_18665) zapD 4647295..4648017 (-) 723 WP_274277525.1 cell division protein ZapD -
  PUB83_RS21200 (PUB83_18670) coaE 4648055..4648669 (-) 615 WP_274277526.1 dephospho-CoA kinase -
  PUB83_RS21205 (PUB83_18675) pilD 4648689..4649561 (-) 873 WP_274277527.1 A24 family peptidase Machinery gene
  PUB83_RS21210 (PUB83_18680) pilC 4649584..4650825 (-) 1242 WP_017409439.1 type II secretion system F family protein Machinery gene
  PUB83_RS21215 (PUB83_18685) pilB 4650941..4652656 (-) 1716 WP_259231738.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  PUB83_RS21220 (PUB83_18690) - 4652647..4653087 (-) 441 WP_226032467.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PUB83_RS21225 (PUB83_18695) nadC 4653410..4654267 (-) 858 WP_274277528.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PUB83_RS21230 (PUB83_18700) - 4654273..4654743 (-) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  PUB83_RS21235 (PUB83_18705) ampD 4654891..4655463 (+) 573 WP_274277529.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PUB83_RS21240 (PUB83_18710) pdhR 4655739..4656503 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62883.40 Da        Isoelectric Point: 5.4342

>NTDB_id=788335 PUB83_RS21215 WP_259231738.1 4650941..4652656(-) (pilB) [Aeromonas hydrophila subsp. hydrophila strain PSKL.DP]
MPIMTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDE
SEISELEVSDEGSRLDESVNTTDDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRF
SARLKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLEL
GFTQQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVT
SLAEINRVTTN

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=788335 PUB83_RS21215 WP_259231738.1 4650941..4652656(-) (pilB) [Aeromonas hydrophila subsp. hydrophila strain PSKL.DP]
CTGCCCATAATGACATCTAGCCCCAATAGCGGCCTGGCATTAAGCCTGGCCGCTTCTTCCCTGCTCAGTGAGTCCGACTC
ACAACGCTACCTGAGCCAAGCCAAGGCACAGCGCAAACCTTTTGTGACTTTTCTGATCGAAAACGAAATCCTCGACAGCA
AGGCACTGGCTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTAGATCTTGCCGCCTTCGACCTGGCCGAGATCCCG
CAGAAATACCTCAATCAGAAGCTGATTGAGAAACATCACGTCCTGCCCATCTACACCCAGGGGCACACCCTCTATATCGC
CATGTCAGATCCTACCAATGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTGGTAG
AAGAGAACAAACTCACTACTGCCATCGGCAAGCTGATGGAAAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAA
TCCGAGATCTCCGAGCTGGAGGTCTCGGACGAGGGATCACGACTCGATGAGAGCGTCAATACCACCGACGATGACGCCCC
CATCGTCAAATACATCAACAAGATCATGATGGATGCCATCAAGCGCGGTGCCTCTGACCTTCACTTCGAGCCCTACGAGA
CCAAATACCGCATCCGTTTTCGGATAGACGGGATCCTGCATGAAATAGCGACGCCACCAGTCAATCTGGCCAACCGCTTC
TCAGCCCGCCTCAAGGTGATGGCACGGCTCGACATCGCCGAGCGGCGACTGCCCCAGGATGGTCGCATCAAGCTCAAGCT
GTCGCGCAACAAGTCGATGGACATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCG
ACTCCTCCGCTGCACGCCTCAATATCGAGCAGCTCGGTTTCGACGATCGGCAGAAAGCGCAATACCTGCGTGCCCTCTCG
AAGCCTCAGGGCATGATCCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTTTCCCTCTATACCGGTCTCAATATTCT
CAATACCACCGAGGTCAATATCTCCACAGCCGAAGATCCGGTGGAAATCAACCTGCCCGGCGTCAATCAGGTGCAGGTCA
ATCCCAAGGCCGGACTGACCTTCGCCAGCGCCCTGCGCTCTTTCTTGCGCCAAGACCCCGATGTAGTCATGGTGGGTGAA
ATCCGGGATCTGGAAACAGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTATCCACTCTGCACACCAA
CTCGGCGGCGGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCA
TGGCTCAGCGGCTGGCGCGTAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTACCCGAGGCTGAGCTGCTTGAGCTG
GGTTTTACCCAGCAGCAGCTGGCCGCTGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCGGGTGGCTACAA
GGGCCGGGTCGGCATTTACGAAATCATGCTAATGTCTGAAAATATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGC
AAATTGCCGCCATTGCCCAGAAGGAGGGCATGCGCACCTTGCGCACCTCCGGCCTTGAGAAGGCCCGCCTGGGGGTGACC
AGTCTGGCGGAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.401

100

0.557
  pilB Acinetobacter baylyi ADP1

55.323

100

0.555
  pilB Legionella pneumophila strain ERS1305867

54.513

98.949

0.539
  pilB Vibrio cholerae strain A1552

54.159

98.949

0.536
  pilB Vibrio campbellii strain DS40M4

51.943

99.124

0.515
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.424

0.506
  pilF Neisseria gonorrhoeae MS11

46.454

98.774

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

96.848

0.377
  pilF Thermus thermophilus HB27

40.152

92.469

0.371