Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   PO765_RS23770 Genome accession   NZ_CP117848
Coordinates   5287679..5288848 (+) Length   389 a.a.
NCBI ID   WP_331693087.1    Uniprot ID   -
Organism   Pandoraea sputorum strain B09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5282679..5293848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PO765_RS23740 cgtA 5282784..5283914 (-) 1131 WP_331693088.1 Obg family GTPase CgtA -
  PO765_RS23745 rpmA 5284064..5284324 (-) 261 WP_039393311.1 50S ribosomal protein L27 -
  PO765_RS23750 rplU 5284353..5284664 (-) 312 WP_039393314.1 50S ribosomal protein L21 -
  PO765_RS23755 - 5284992..5285921 (+) 930 WP_331693288.1 polyprenyl synthetase family protein -
  PO765_RS23765 - 5286245..5287537 (+) 1293 WP_039393320.1 HlyC/CorC family transporter -
  PO765_RS23770 pilF 5287679..5288848 (+) 1170 WP_331693087.1 GspE/PulE family protein Machinery gene
  PO765_RS23775 - 5288987..5290213 (+) 1227 WP_331693086.1 type II secretion system F family protein -
  PO765_RS23780 - 5290210..5291070 (+) 861 WP_331693085.1 A24 family peptidase -
  PO765_RS23785 coaE 5291067..5291681 (+) 615 WP_331693083.1 dephospho-CoA kinase -
  PO765_RS23790 zapD 5291857..5292612 (+) 756 WP_039393327.1 cell division protein ZapD -
  PO765_RS23795 yacG 5292617..5292811 (+) 195 WP_039393329.1 DNA gyrase inhibitor YacG -
  PO765_RS23800 - 5292948..5293382 (-) 435 WP_150777649.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 389 a.a.        Molecular weight: 41824.02 Da        Isoelectric Point: 7.1647

>NTDB_id=787905 PO765_RS23770 WP_331693087.1 5287679..5288848(+) (pilF) [Pandoraea sputorum strain B09]
MLRPTIASDDALPSTGTPASTTPLQRLEQILSEAIRAGASDIHFEPMAQRFRVRLRVDGRLQEHGDVPLTARDMLVSRLK
VLANLDIAQKRLPQDGRMVWREAGEPVECRVSALPTLHGEKLVVRLLDGAKVPLSLEGLGYSPKQYLALTQAIARPHGLI
LLTGPTGSGKTVSLYSCLRRLNDASRNIVTIEDPVEIRLAGITQVNLNERAGLDFATALRAFLRQDPDVLVVGEIRDAQT
AEIAIQAAQTGHLVFATVHANDAPSTLARLFDLGVAPFNLSSTLLLVSAQRLLRRRCPAGCEPSRTTTETGLADTCMRCH
GTGFAGRIGAFQVMPISATQAINIAQARSPHELAAQACSEGVMTLREAGLWHVDAGAVAQEDVDATLPA

Nucleotide


Download         Length: 1170 bp        

>NTDB_id=787905 PO765_RS23770 WP_331693087.1 5287679..5288848(+) (pilF) [Pandoraea sputorum strain B09]
ATGCTGCGTCCAACCATCGCCTCCGACGACGCTCTGCCGTCCACCGGCACCCCGGCGTCCACCACACCTCTGCAACGCCT
TGAGCAAATACTGAGCGAAGCGATTCGTGCGGGCGCTTCCGATATCCATTTCGAACCGATGGCGCAGCGCTTTCGGGTCC
GTCTGCGTGTCGACGGTCGCCTGCAGGAGCATGGCGACGTTCCGCTGACAGCGCGCGACATGCTGGTCTCGCGTCTGAAG
GTGCTTGCCAATCTCGACATCGCACAAAAGCGCTTGCCGCAGGACGGACGCATGGTGTGGCGCGAAGCTGGTGAACCGGT
CGAGTGTCGCGTGAGTGCGCTTCCCACCCTGCACGGCGAGAAACTCGTGGTGCGCCTGCTCGATGGCGCGAAAGTCCCTC
TCTCGCTTGAGGGTCTCGGCTATTCGCCGAAGCAATACCTCGCACTCACGCAAGCGATTGCCCGGCCACATGGCCTGATC
TTGCTGACGGGCCCAACGGGCAGTGGAAAGACCGTCTCGCTCTATAGTTGCCTGCGACGTCTGAACGACGCGTCGCGCAA
CATCGTCACCATCGAGGACCCGGTGGAAATTCGCTTGGCCGGTATCACGCAGGTCAATCTCAACGAACGCGCCGGCCTCG
ACTTTGCAACGGCTTTACGTGCGTTTCTCCGTCAGGATCCAGATGTCCTCGTCGTGGGCGAAATCCGCGATGCGCAGACC
GCCGAAATCGCCATACAGGCCGCGCAGACCGGTCACCTTGTGTTCGCCACCGTTCACGCCAACGACGCGCCGAGCACGCT
CGCGCGGCTCTTCGATCTCGGTGTCGCCCCGTTCAATCTGTCGTCGACATTACTGCTGGTCAGCGCACAGCGTCTGCTTA
GGCGACGATGCCCGGCCGGGTGCGAGCCGTCCCGCACGACGACTGAAACAGGCCTCGCTGACACCTGCATGCGGTGTCAC
GGCACCGGTTTTGCCGGACGCATCGGCGCGTTTCAGGTGATGCCGATCAGCGCCACGCAGGCTATCAACATTGCACAGGC
GCGCAGTCCGCACGAGCTGGCGGCGCAGGCATGCAGCGAAGGCGTCATGACGCTGCGAGAAGCCGGGTTATGGCATGTCG
ATGCAGGGGCTGTCGCTCAGGAGGATGTCGATGCGACGCTGCCCGCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(29-295)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

48.462

100

0.486
  pilB Vibrio cholerae strain A1552

47.545

99.486

0.473
  pilB Vibrio campbellii strain DS40M4

47.709

95.373

0.455
  pilB Vibrio parahaemolyticus RIMD 2210633

46.194

97.943

0.452
  pilB Acinetobacter baumannii D1279779

43.89

100

0.452
  pilB Legionella pneumophila strain ERS1305867

43.035

100

0.445
  pilB Acinetobacter baylyi ADP1

45.526

97.686

0.445
  pilB/pilB1 Synechocystis sp. PCC 6803

40.587

100

0.427
  pilF Thermus thermophilus HB27

41.327

100

0.416
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.114

96.915

0.398
  pilB Haemophilus influenzae 86-028NP

42.265

93.059

0.393
  pilB Haemophilus influenzae Rd KW20

46.602

79.434

0.37
  pilB Glaesserella parasuis strain SC1401

39.024

94.859

0.37