Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PGW99_RS01280 Genome accession   NZ_CP117520
Coordinates   251835..253544 (-) Length   569 a.a.
NCBI ID   WP_273778280.1    Uniprot ID   -
Organism   Acinetobacter sp. GSS19     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 246835..258544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PGW99_RS01250 - 246990..247316 (+) 327 WP_273778274.1 pyrimidine/purine nucleoside phosphorylase -
  PGW99_RS01255 rlmB 247422..248168 (+) 747 WP_273778275.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  PGW99_RS01260 - 248204..249115 (+) 912 WP_273778276.1 DMT family transporter -
  PGW99_RS01265 coaE 249109..249714 (-) 606 WP_273778277.1 dephospho-CoA kinase -
  PGW99_RS01270 pilD 249716..250576 (-) 861 WP_273778278.1 A24 family peptidase Machinery gene
  PGW99_RS01275 pilC 250576..251802 (-) 1227 WP_273778279.1 type II secretion system F family protein Machinery gene
  PGW99_RS01280 pilB 251835..253544 (-) 1710 WP_273778280.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PGW99_RS01285 tpiA 253833..254627 (+) 795 WP_273778281.1 triose-phosphate isomerase -
  PGW99_RS01290 secG 254640..254969 (+) 330 WP_273778283.1 preprotein translocase subunit SecG -
  PGW99_RS01310 rimP 255591..256115 (+) 525 WP_273778284.1 ribosome maturation factor RimP -
  PGW99_RS01315 nusA 256150..257634 (+) 1485 WP_273778285.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63296.46 Da        Isoelectric Point: 4.9197

>NTDB_id=786212 PGW99_RS01280 WP_273778280.1 251835..253544(-) (pilB) [Acinetobacter sp. GSS19]
MSVLQGFPKFTGFIRRLVEEHHISAEDMQTAVENAKKAKRDIVAYLIEHHGISPLILADTISREFGEPLFDLAVYDAGLL
PRDLVEDKLLTKHHIAPLFKRGNTLYVATSNPTNIEALDAIRFNSKLNIESVIVEYDKLSQLLSKQFTEEDTFNFDGEEL
DIDIDDSSQSDDDDDDGPQGDEAPIVKYINKLLVDAIRMGASDLHFEPYEKIYRVRYRVDGVLRQIATPPLQLANRLASR
LKVMSQMDISEKRIPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKALFMEALEKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAPVEVPQNSLLEMGFT
EEDLANPEFKLYQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASTKLGFDNLRRSGLKKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=786212 PGW99_RS01280 WP_273778280.1 251835..253544(-) (pilB) [Acinetobacter sp. GSS19]
ATGTCAGTATTGCAAGGATTTCCTAAATTTACAGGATTTATTCGTCGATTAGTCGAGGAACATCATATCTCAGCTGAAGA
TATGCAAACTGCTGTCGAAAATGCCAAAAAGGCCAAACGAGATATTGTCGCCTACCTCATTGAGCATCATGGTATTTCAC
CTCTCATTCTTGCCGATACCATTTCACGCGAATTTGGCGAGCCGCTGTTCGACCTTGCAGTTTATGATGCAGGCTTACTA
CCTCGCGATCTGGTTGAAGACAAACTGCTGACCAAACATCATATTGCACCGCTGTTTAAACGTGGCAATACCTTGTATGT
CGCAACCAGCAACCCAACCAATATCGAAGCACTCGATGCCATCCGCTTCAATAGCAAACTGAACATTGAATCAGTCATTG
TTGAATATGACAAACTGAGTCAACTGCTCAGCAAACAGTTCACCGAAGAAGATACCTTTAATTTTGATGGTGAAGAGCTC
GATATTGATATTGATGATTCATCACAATCTGATGATGACGATGATGACGGCCCACAAGGTGATGAAGCTCCGATTGTCAA
ATACATCAACAAACTATTGGTTGATGCCATCCGTATGGGGGCTTCGGATCTGCATTTTGAACCGTATGAAAAAATCTATC
GGGTGCGTTACCGAGTCGATGGAGTCTTGCGTCAGATCGCAACGCCTCCATTACAATTGGCGAACCGTCTGGCTTCACGT
TTAAAAGTCATGTCACAAATGGACATTTCGGAGAAACGGATTCCTCAAGATGGCCGTATCAAACTGAAATTATCCAAAAC
CAAAGCGATTGATTTCCGTGTCAACTCCCTTCCGACGCTATTTGGGGAGAAACTGGTTCTACGTATTCTCGATCCATCCA
GTGCCATGCTAGGGATTGATGCGCTAGGTTATGAACCCGATCAAAAAGCCCTGTTTATGGAAGCGCTGGAAAAACCGCAA
GGCATGTTATTGATTACCGGTCCGACGGGTTCCGGTAAAACTGTTTCTTTATATACCGGCTTGAATATCCTCAATCGTGA
GGACACCAACATTTCCACGGCGGAAGATCCCGTCGAAATTAACCTCGAAGGCATTAACCAGGTCAATGTAAACAATAAAG
TAGGGCTAACCTTTTCTGCAGCATTAAAATCTTTCTTACGTCAGGATCCGGACATTATCATGGTCGGTGAGATTCGTGAT
CTGGAAACCGCAGAAATCGCGATTAAGGCGGCACAGACCGGTCACATGGTGATGTCGACACTGCATACCAATAGTGCACC
CGAAACGCTGACCCGTTTACGTAACATGGGTGTCCCTTCTTTCAATATCGCCACTTCGGTTAATCTGGTGATTGCGCAAC
GTTTGGCACGTCGGTTATGCAGTCAATGCAAGGCTCCGGTCGAGGTTCCTCAAAATAGCCTGCTCGAAATGGGCTTCACT
GAAGAAGATCTAGCTAATCCGGAATTTAAACTCTACCAACCGGTGGGTTGTTCAGAGTGTCGCGAAGGTTATAAAGGCCG
GGTCGGGATTTATGAGGTCATGAAAGTGACCCCTGAGATTTCAAAGATTATCATGGAAGATGGCAATGCGTTGGAAATTG
CAGCGGCATCGACAAAATTGGGGTTTGATAATCTGCGACGTTCAGGCTTAAAAAAAGTCATGCAAGGGGTCACTTCACTA
CAAGAAGTGAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

82.105

100

0.822
  pilB Acinetobacter baylyi ADP1

79.123

100

0.793
  pilB Legionella pneumophila strain ERS1305867

54.428

95.255

0.518
  pilF Neisseria gonorrhoeae MS11

51.068

98.77

0.504
  pilB Vibrio cholerae strain A1552

47.979

100

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

51.83

91.213

0.473
  pilB Vibrio campbellii strain DS40M4

50.772

91.037

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

34.783

100

0.38
  pilF Thermus thermophilus HB27

37.043

100

0.374