Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PGW97_RS15190 Genome accession   NZ_CP117517
Coordinates   3321529..3323196 (-) Length   555 a.a.
NCBI ID   WP_047887157.1    Uniprot ID   A0A0J1H1A6
Organism   Photobacterium sp. GSS17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3316529..3328196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PGW97_RS15165 - 3316896..3317165 (+) 270 WP_273857637.1 DUF2970 domain-containing protein -
  PGW97_RS15170 - 3317238..3318065 (-) 828 WP_217391482.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  PGW97_RS15175 - 3318173..3319684 (-) 1512 WP_273857640.1 AAA family ATPase -
  PGW97_RS15180 aroB 3319694..3320776 (-) 1083 WP_273857641.1 3-dehydroquinate synthase -
  PGW97_RS15185 aroK 3320797..3321315 (-) 519 WP_047887156.1 shikimate kinase AroK -
  PGW97_RS15190 pilQ 3321529..3323196 (-) 1668 WP_047887157.1 type IV pilus secretin PilQ family protein Machinery gene
  PGW97_RS15195 - 3323281..3323811 (-) 531 WP_047887158.1 pilus assembly protein PilP -
  PGW97_RS15200 - 3323808..3324398 (-) 591 WP_273857644.1 type 4a pilus biogenesis protein PilO -
  PGW97_RS15205 - 3324391..3324969 (-) 579 WP_047887160.1 PilN domain-containing protein -
  PGW97_RS15210 pilM 3324966..3325967 (-) 1002 WP_273857647.1 type IV pilus assembly protein PilM -

Sequence

Protein


Download         Length: 555 a.a.        Molecular weight: 60696.47 Da        Isoelectric Point: 4.8279

>NTDB_id=786192 PGW97_RS15190 WP_047887157.1 3321529..3323196(-) (pilQ) [Photobacterium sp. GSS17]
MLWALMQSSVSFAAAENRIQAIDFTRSTAGHGVLKVDLANARSVVDMRRAGEKLYVDVHDTEIPEDMIYTLDVKEFATRV
QTIETFRTERGVRLVLSMNGTYAYDYTSKDKVLTVTVEKVAKPSSAKAAPSSISYNSKPISINFQDVPVRNVLQLIAEYN
DFNLVVSDSVQGNLTLRLDDVPWKQVLDIILKSKGLDKREQGSVVMIAPKEELAREEQKQLESKRKEEELASLRSEIIQI
NYAKASELGELLQGSGEGVSMLSPRGSLHIDERTNSLIINDIAESLANIRDIVTALDIPVQQVQIEARVVTVNEGDIDEL
GVRWGMLSRNGNNTIGSSIEGNLVSEGLLDLADNRVDQVGDFLNVNLGATKAGASSIAFQVAKIGDVLLDLELSALQAEQ
KAEIISSPRLMTTNKKMAYIEQGTEIPYLEASSSGAVSVGFKKAVLSLMVTPQITPDGKLVLDLVVTQDKKGDVVKTGTG
EAIAIDTQRIGTQVLVENGETVVLGGIYQHSLTNMVRKVPLLGDLPVLGALFRHKLEELGKRELLIFVTPKIINQ

Nucleotide


Download         Length: 1668 bp        

>NTDB_id=786192 PGW97_RS15190 WP_047887157.1 3321529..3323196(-) (pilQ) [Photobacterium sp. GSS17]
ATGTTATGGGCCCTGATGCAAAGTTCAGTCTCATTTGCTGCCGCAGAAAACCGAATTCAGGCAATCGACTTTACACGCAG
TACGGCCGGGCACGGGGTCCTGAAAGTCGATCTGGCAAACGCCCGTTCCGTGGTCGATATGCGCCGGGCTGGCGAGAAGC
TGTATGTTGATGTGCATGACACGGAGATTCCGGAGGACATGATTTATACCCTCGACGTGAAAGAGTTCGCGACCCGGGTA
CAAACCATCGAAACCTTCCGAACCGAGCGGGGGGTGCGTTTGGTGCTCTCAATGAACGGCACTTATGCCTATGACTACAC
CAGCAAGGATAAAGTGCTGACGGTGACCGTCGAGAAAGTGGCAAAACCAAGCTCAGCCAAGGCCGCGCCGTCATCGATTT
CTTATAACAGCAAACCCATCTCTATCAACTTCCAGGATGTGCCAGTGCGAAACGTGCTGCAGCTGATCGCTGAGTACAAC
GATTTCAACCTGGTGGTCTCGGATTCGGTGCAGGGCAACCTGACGCTGCGGTTGGACGACGTACCATGGAAGCAGGTGCT
GGATATCATTTTGAAATCCAAAGGGCTGGACAAGCGTGAACAGGGCAGTGTGGTCATGATTGCCCCGAAAGAAGAGCTGG
CGCGAGAAGAGCAGAAGCAGCTGGAGTCGAAGCGTAAAGAAGAGGAACTGGCTTCACTGCGCTCGGAGATTATCCAGATT
AACTACGCCAAGGCATCGGAGCTGGGCGAGCTGCTGCAGGGCAGCGGTGAAGGGGTGAGCATGTTGTCACCGCGTGGCTC
ACTGCATATTGATGAACGAACTAACTCTCTGATCATTAATGATATTGCTGAAAGTCTGGCGAATATCCGCGATATCGTGA
CGGCGCTCGATATCCCAGTGCAGCAGGTCCAAATTGAAGCCCGGGTGGTCACGGTGAACGAAGGTGATATTGACGAGTTG
GGGGTTCGCTGGGGGATGCTGAGTCGTAATGGCAACAATACGATCGGTAGCTCCATTGAAGGCAATTTGGTGTCGGAAGG
GCTGCTTGATCTGGCAGATAACCGGGTGGATCAGGTCGGGGATTTCCTCAATGTTAATTTGGGAGCCACCAAAGCGGGGG
CGTCAAGCATCGCTTTCCAGGTCGCCAAAATCGGCGATGTGTTACTGGATCTGGAATTATCGGCCCTGCAGGCGGAGCAG
AAAGCGGAAATCATTTCCAGTCCGCGCCTGATGACCACCAATAAAAAAATGGCCTATATCGAGCAGGGAACCGAAATTCC
GTACTTGGAAGCATCATCCAGCGGCGCGGTATCGGTGGGCTTTAAAAAGGCCGTGCTCAGTCTGATGGTGACCCCGCAGA
TTACTCCGGACGGCAAGCTGGTGCTGGATTTGGTCGTTACCCAGGATAAGAAGGGGGATGTCGTAAAAACCGGGACCGGC
GAAGCGATTGCGATTGATACCCAGCGTATCGGGACCCAGGTTCTGGTTGAAAATGGCGAGACCGTTGTTTTGGGGGGAAT
CTACCAGCATTCGCTGACAAACATGGTGCGTAAAGTGCCATTGCTGGGGGATTTACCGGTATTAGGCGCCTTGTTCCGCC
ATAAGCTGGAAGAGCTGGGCAAGCGTGAATTGCTGATTTTTGTGACGCCAAAGATTATTAATCAATAG

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(162-210)

Bacterial type II and III secretion system protein (Secretin)

(395-553)

Bacterial type II/III secretion system short domain (Secretin_N)

(236-302)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0J1H1A6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

61.21

100

0.62
  pilQ Vibrio cholerae strain A1552

61.21

100

0.62
  pilQ Vibrio campbellii strain DS40M4

61.151

100

0.613
  comQ Acinetobacter baylyi ADP1

40.696

100

0.422
  pilQ Legionella pneumophila strain Lp02

41.728

98.018

0.409
  pilQ Legionella pneumophila strain ERS1305867

41.728

98.018

0.409
  pilQ Acinetobacter baumannii D1279779

38.605

100

0.409
  pilQ Acinetobacter baumannii strain A118

38.605

100

0.409
  pilQ Pseudomonas aeruginosa PAK

40.072

100

0.404