Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PGW97_RS14350 Genome accession   NZ_CP117517
Coordinates   3159739..3161439 (+) Length   566 a.a.
NCBI ID   WP_273857549.1    Uniprot ID   -
Organism   Photobacterium sp. GSS17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3154739..3166439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PGW97_RS14330 pdhR 3156013..3156786 (-) 774 WP_047886938.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  PGW97_RS14335 ampD 3157083..3157646 (-) 564 WP_273857548.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PGW97_RS14340 nadC 3157938..3158825 (+) 888 WP_273859675.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PGW97_RS14345 - 3159296..3159739 (+) 444 WP_217391891.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PGW97_RS14350 pilB 3159739..3161439 (+) 1701 WP_273857549.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PGW97_RS14355 pilC 3161493..3162713 (+) 1221 WP_273857550.1 type II secretion system F family protein Machinery gene
  PGW97_RS14360 pilD 3163012..3163881 (+) 870 WP_273857551.1 A24 family peptidase Machinery gene
  PGW97_RS14365 coaE 3163886..3164500 (+) 615 WP_273857552.1 dephospho-CoA kinase -
  PGW97_RS14370 zapD 3164525..3165262 (+) 738 WP_047886946.1 cell division protein ZapD -
  PGW97_RS14375 yacG 3165271..3165489 (+) 219 WP_047887028.1 DNA gyrase inhibitor YacG -
  PGW97_RS14380 mutT 3165583..3165981 (-) 399 WP_047886947.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62616.63 Da        Isoelectric Point: 5.5203

>NTDB_id=786187 PGW97_RS14350 WP_273857549.1 3159739..3161439(+) (pilB) [Photobacterium sp. GSS17]
MHTNLASILHQADLLSLVEQQQVVDQVQSEGISVPSALLQLGIIGSAELARQLEQLFALPLVDVHQYDYTECCKSLNLRD
LVLRHKVLPLYNTDISIFIGLSDPTRIDVLDEFRFATGKNIEPLLLDHKQLEAAIRRVYGSDIKGAGSNKTQRSVSDADL
MELVDLTDTELADNATDLTSDNAPVTRYINQVLLDALRKSASDIHFEPYEESYRIRFRCDGILHLHASPPANLSRRLSTR
LKVMSRLNIAERRQPQDGRIKLKLSENVAVDLRVSTLPTLWGEKVVLRILDSSSANLDIDILGYTEPQKAAYMSALQRPQ
GMILMTGPTGSGKTVSLYTGLKILNTEERNISTAEDPVEINLPGINQVQIRPTVGLGFAEALRSFLRQDPDVVMVGEIRD
LETGSIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGIENFNLASSLSLIIAQRLARRLCGHCKQPDDPDRFIRDTLAIP
DNATIFKASPTGCDECNKGYLGRVGIYEVMPFSRELAEALMAGANTLELEDIACRQGMRTLQQSGIEKLCEGITSLKELQ
RVLHFS

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=786187 PGW97_RS14350 WP_273857549.1 3159739..3161439(+) (pilB) [Photobacterium sp. GSS17]
ATGCACACCAACCTAGCCTCCATCCTTCATCAGGCAGACCTGCTCAGTCTGGTGGAGCAACAACAAGTGGTGGATCAGGT
TCAGTCAGAGGGCATTTCGGTGCCCTCTGCACTGTTACAACTGGGCATCATCGGCAGCGCCGAACTGGCCCGGCAACTGG
AACAGCTGTTCGCCCTGCCTTTGGTTGATGTCCATCAGTACGATTACACGGAATGTTGCAAAAGCCTAAACCTACGGGAT
CTGGTGTTACGCCACAAAGTGCTGCCGCTGTACAACACGGACATCAGTATTTTTATTGGCCTGAGCGATCCAACCCGCAT
TGATGTGCTGGATGAGTTCCGCTTTGCCACCGGCAAAAACATTGAGCCGCTATTGCTGGATCATAAGCAGCTGGAAGCGG
CGATCCGCCGGGTATACGGCAGTGACATTAAAGGGGCTGGCTCCAACAAAACACAGCGCAGTGTCAGTGATGCGGATCTG
ATGGAGCTGGTGGATCTGACCGACACTGAACTGGCTGACAACGCCACAGATCTCACCAGTGATAACGCCCCGGTAACCCG
CTATATCAACCAGGTGCTGCTGGACGCCCTGCGTAAAAGCGCTTCCGATATCCATTTTGAACCCTATGAAGAGAGCTATC
GGATCCGTTTTCGCTGCGACGGGATTTTGCATCTGCACGCCTCCCCACCGGCCAATTTGTCCCGTCGCTTATCCACCCGG
CTGAAGGTCATGTCCAGGCTCAATATTGCCGAGCGGCGCCAGCCTCAGGATGGCCGGATCAAGCTCAAATTGTCCGAAAA
TGTGGCCGTGGATTTGCGGGTTTCCACACTGCCGACCCTGTGGGGTGAAAAGGTGGTGCTGCGGATTCTTGACAGCTCCA
GTGCCAATCTGGATATCGATATTTTGGGTTATACCGAGCCCCAAAAAGCCGCATACATGAGTGCCCTTCAGCGCCCTCAG
GGCATGATTCTGATGACCGGACCGACGGGCAGTGGCAAAACTGTCTCCCTGTATACCGGCCTCAAGATCCTGAACACCGA
GGAACGCAATATCTCCACCGCCGAAGACCCGGTGGAAATTAACCTGCCGGGCATAAATCAGGTACAGATTCGCCCAACCG
TCGGGCTTGGGTTTGCCGAAGCACTGCGCTCATTCTTGCGCCAAGATCCGGATGTCGTCATGGTCGGGGAAATCAGGGAT
CTGGAAACCGGCTCTATTGCCGTGAAAGCAGCTCAGACTGGCCACCTGGTGTTGTCGACACTGCACACCAACTCGGCGGC
AGAAACCATTACCCGCCTGACCAATATGGGGATCGAGAACTTCAACCTGGCATCGTCATTGAGCCTGATCATTGCTCAGC
GGCTGGCCCGCCGCCTTTGCGGACATTGCAAGCAGCCTGATGACCCGGATCGTTTTATCAGAGATACCCTCGCGATTCCC
GACAATGCCACCATTTTCAAAGCCAGCCCAACAGGGTGTGATGAGTGTAACAAAGGCTATCTGGGACGGGTCGGTATCTA
CGAGGTCATGCCCTTTTCACGAGAGCTCGCCGAAGCCTTAATGGCAGGAGCCAATACACTGGAGCTGGAAGACATTGCCT
GTCGGCAAGGCATGAGAACCCTGCAACAGTCAGGTATCGAGAAGCTATGCGAAGGCATCACCAGTTTAAAAGAGTTACAA
CGCGTACTGCACTTTAGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

62.301

99.823

0.622
  pilB Vibrio parahaemolyticus RIMD 2210633

61.062

99.823

0.61
  pilB Vibrio campbellii strain DS40M4

60.247

100

0.602
  pilB Acinetobacter baumannii D1279779

52.874

92.226

0.488
  pilB Legionella pneumophila strain ERS1305867

49.907

95.23

0.475
  pilB Acinetobacter baylyi ADP1

52.242

90.636

0.474
  pilF Neisseria gonorrhoeae MS11

43.915

100

0.44
  pilB/pilB1 Synechocystis sp. PCC 6803

37.943

100

0.398
  pilF Thermus thermophilus HB27

36.508

100

0.366