Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSH75_RS04150 Genome accession   NZ_CP117448
Coordinates   917292..918500 (-) Length   402 a.a.
NCBI ID   WP_305469864.1    Uniprot ID   -
Organism   Pseudomonas simiae strain FP2084     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 912292..923500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH75_RS04110 (PSH75_04110) - 913210..913383 (-) 174 WP_010213243.1 DUF3094 family protein -
  PSH75_RS04115 (PSH75_04115) - 913438..914067 (-) 630 WP_010213242.1 DUF1780 domain-containing protein -
  PSH75_RS04120 (PSH75_04120) - 914171..914638 (+) 468 WP_305469862.1 FAD/FMN-containing dehydrogenase -
  PSH75_RS04125 (PSH75_04125) - 914635..915324 (+) 690 WP_042571059.1 energy-coupling factor ABC transporter permease -
  PSH75_RS04130 (PSH75_04130) - 915400..915615 (+) 216 WP_003171680.1 hypothetical protein -
  PSH75_RS04135 (PSH75_04135) yacG 915612..915812 (-) 201 WP_003188437.1 DNA gyrase inhibitor YacG -
  PSH75_RS04140 (PSH75_04140) coaE 915809..916432 (-) 624 WP_010213235.1 dephospho-CoA kinase -
  PSH75_RS04145 (PSH75_04145) pilD 916429..917295 (-) 867 WP_305469863.1 A24 family peptidase Machinery gene
  PSH75_RS04150 (PSH75_04150) pilC 917292..918500 (-) 1209 WP_305469864.1 type II secretion system F family protein Machinery gene
  PSH75_RS04155 (PSH75_04155) - 918694..919116 (+) 423 WP_021491914.1 pilin -
  PSH75_RS04160 (PSH75_04160) - 919152..920552 (+) 1401 WP_305469866.1 O-antigen ligase -
  PSH75_RS04170 (PSH75_04170) - 920819..921124 (+) 306 WP_010213230.1 DUF6388 family protein -
  PSH75_RS04175 (PSH75_04175) nadC 921172..922020 (-) 849 WP_010213228.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43414.84 Da        Isoelectric Point: 9.8698

>NTDB_id=785357 PSH75_RS04150 WP_305469864.1 917292..918500(-) (pilC) [Pseudomonas simiae strain FP2084]
MDNASTLYAWEGINRKGRRVSGQTTGHNPALVKAQLRKQGICPGHVRKKSPVLSSLAPSITSTDITLFTRQLATLLKAGI
PLLQAFDIIAEGFDNRALRELVQGLKQAIEAGTSLAEALRKQPRCFDELYCNLVAAGEQAGALETLLERVAIHREKSEQL
KTRIKKAMTYPITVLVVASLVTGVLLVHVVPQFQNLFAGVDGKLPGFTLQVIGLSEFMQKAWWAVALGFLAGGLGLREAY
RRLADFRLWLDSCLLKAPVAGKLLSKSAVARFARTLSTTFAAGVPLLQALDSVAGAAGSGPFRRAIEQMRRDVATGMPLN
QSMLTSGLFPGMAIQMTAIGEESGTLDRMLEKVANHYEADVDNLVDHLTHLMEPLIMVVLGGIVGALVIAMYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=785357 PSH75_RS04150 WP_305469864.1 917292..918500(-) (pilC) [Pseudomonas simiae strain FP2084]
ATGGACAACGCTTCAACGCTCTACGCCTGGGAAGGCATCAACCGCAAAGGACGCAGGGTGTCCGGGCAAACCACCGGACA
CAACCCTGCCCTGGTGAAGGCACAGTTGCGAAAACAGGGCATCTGCCCAGGGCATGTGCGCAAAAAATCCCCAGTGCTGT
CGAGCCTGGCACCCTCGATCACATCAACCGACATCACCCTGTTCACTCGCCAACTGGCCACCCTGCTGAAGGCCGGTATT
CCGCTGCTGCAGGCCTTCGACATCATCGCTGAGGGCTTCGACAACCGCGCCTTGCGTGAGCTTGTGCAAGGGTTGAAACA
GGCCATCGAAGCCGGCACCAGCCTTGCCGAGGCGCTGCGCAAGCAGCCGCGTTGTTTCGACGAGCTGTACTGCAACCTGG
TGGCCGCCGGCGAACAGGCCGGAGCACTTGAAACCTTGCTGGAGCGCGTGGCGATCCACCGGGAAAAGAGCGAGCAGCTA
AAGACGAGGATCAAAAAAGCCATGACCTATCCGATCACCGTGCTGGTGGTCGCCAGCCTCGTCACCGGCGTGCTGCTGGT
CCACGTCGTGCCACAGTTCCAGAACCTGTTTGCCGGGGTCGACGGCAAGCTGCCGGGTTTCACCCTGCAGGTCATCGGCC
TTTCCGAGTTCATGCAGAAAGCCTGGTGGGCCGTGGCGCTGGGCTTTCTTGCGGGAGGCCTGGGGCTGCGCGAAGCGTAT
CGGCGCTTGGCGGATTTCCGTCTATGGCTGGACAGCTGTTTGTTGAAAGCGCCCGTGGCAGGCAAACTGCTGAGCAAATC
CGCCGTGGCCCGCTTCGCCCGCACGCTGTCCACCACGTTTGCCGCCGGCGTTCCGTTACTGCAGGCGCTGGATTCGGTGG
CCGGTGCCGCCGGTTCCGGGCCATTCAGACGGGCAATCGAACAGATGCGACGCGATGTAGCGACTGGCATGCCATTGAAC
CAATCCATGCTCACCAGCGGCCTGTTCCCCGGCATGGCGATCCAGATGACAGCCATCGGCGAAGAGTCGGGCACACTGGA
CCGCATGCTGGAAAAGGTCGCCAACCATTACGAGGCCGATGTGGACAACCTGGTCGATCACCTCACCCACCTCATGGAGC
CCCTGATCATGGTGGTCCTGGGAGGCATTGTCGGCGCATTGGTGATCGCCATGTACCTGCCAGTCTTCCAGTTGGGGAGC
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)

Type II secretion system (T2SS), protein F (T2SSF)

(272-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

60.101

98.507

0.592
  pilC Acinetobacter baumannii D1279779

53.418

98.259

0.525
  pilC Acinetobacter baylyi ADP1

51.768

98.507

0.51
  pilC Legionella pneumophila strain ERS1305867

50.505

98.507

0.498
  pilC Vibrio cholerae strain A1552

41.414

98.507

0.408
  pilG Neisseria gonorrhoeae MS11

41.927

95.522

0.4
  pilC Vibrio campbellii strain DS40M4

40.355

98.01

0.396
  pilG Neisseria meningitidis 44/76-A

41.146

95.522

0.393