Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   POF61_RS01125 Genome accession   NZ_CP116709
Coordinates   189315..190286 (+) Length   323 a.a.
NCBI ID   WP_000250801.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain CNCTC 10/84 sCas9revertant     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 184315..195286
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF61_RS01115 (POF61_01115) rpoC 185014..188664 (+) 3651 WP_000228731.1 DNA-directed RNA polymerase subunit beta' -
  POF61_RS01120 (POF61_01120) - 188778..189143 (+) 366 WP_000285373.1 DUF1033 family protein -
  POF61_RS01125 (POF61_01125) comYA 189315..190286 (+) 972 WP_000250801.1 competence type IV pilus ATPase ComGA Machinery gene
  POF61_RS01130 (POF61_01130) comYB 190180..191223 (+) 1044 WP_228313184.1 competence type IV pilus assembly protein ComGB Machinery gene
  POF61_RS01135 (POF61_01135) comGC/cglC 191220..191549 (+) 330 WP_000793380.1 competence type IV pilus major pilin ComGC Machinery gene
  POF61_RS01140 (POF61_01140) comYD 191524..191937 (+) 414 WP_000244259.1 competence type IV pilus minor pilin ComGD Machinery gene
  POF61_RS01145 (POF61_01145) comGE 191909..192208 (+) 300 WP_038406052.1 competence type IV pilus minor pilin ComGE -
  POF61_RS01150 (POF61_01150) comGF 192162..192623 (+) 462 WP_001874060.1 competence type IV pilus minor pilin ComGF -
  POF61_RS01155 (POF61_01155) comGG 192601..192972 (+) 372 WP_000601105.1 competence type IV pilus minor pilin ComGG -
  POF61_RS01160 (POF61_01160) comYH 193087..194061 (+) 975 WP_001008567.1 class I SAM-dependent methyltransferase Machinery gene
  POF61_RS01165 (POF61_01165) - 194093..195286 (+) 1194 WP_000047534.1 acetate kinase -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 36854.34 Da        Isoelectric Point: 6.8468

>NTDB_id=779350 POF61_RS01125 WP_000250801.1 189315..190286(+) (comYA) [Streptococcus agalactiae strain CNCTC 10/84 sCas9revertant]
MVQSLAKHVIHQAVEVNAQDIYIIPKGDCYELYMRIDDERRFIDVFEFNRMASLISHFKFVAGMNVGEKRRSQLGSCDYE
LSEGRLVSLRLSSVGDYRGQESLVIRILYSGHQDLKYWFDNIKQMKEVLGTRGLYLFSGPVGSGKTTLMYQLASEVFKNK
QIITIEDPVEIKNDKMLQLQLNEDIGMTYDALIKLSLRHRPDILIIGEIRDQATARAVIRASLTGVMVFSTIHAKSIPGV
YDRLIELGVNYQELENSLKLIAYQRLIGGGSLIDFETSNFKKHSSDKWNRQVDILAEEGYISKKQAQVEKIIPQETTESS
PTF

Nucleotide


Download         Length: 972 bp        

>NTDB_id=779350 POF61_RS01125 WP_000250801.1 189315..190286(+) (comYA) [Streptococcus agalactiae strain CNCTC 10/84 sCas9revertant]
ATGGTTCAATCATTAGCAAAGCACGTCATTCATCAGGCAGTAGAAGTAAATGCTCAAGATATTTATATCATTCCAAAAGG
TGATTGTTATGAACTCTATATGCGTATTGATGATGAAAGGCGGTTTATTGATGTTTTTGAGTTTAATAGGATGGCTAGTC
TTATTAGTCACTTTAAATTTGTGGCAGGCATGAACGTTGGAGAAAAAAGACGAAGTCAATTAGGTTCTTGTGACTATGAA
CTGTCAGAGGGAAGACTGGTTTCATTACGACTATCGAGTGTGGGAGATTATCGTGGTCAAGAATCTTTAGTTATTCGTAT
TTTGTATTCAGGTCATCAGGACTTAAAATATTGGTTTGATAATATAAAGCAAATGAAGGAAGTACTGGGTACAAGAGGGC
TATATCTTTTTTCCGGCCCTGTGGGGAGTGGTAAAACAACTCTCATGTATCAATTAGCTTCAGAAGTATTTAAAAATAAG
CAAATTATCACGATTGAAGATCCGGTAGAAATCAAGAATGACAAGATGTTACAACTCCAATTGAATGAGGATATTGGAAT
GACTTATGATGCTTTAATCAAACTGTCTTTACGGCATCGTCCAGATATTTTAATTATCGGAGAGATTAGAGATCAAGCGA
CGGCTCGTGCTGTTATTCGTGCAAGTTTAACGGGAGTGATGGTTTTTTCTACTATTCATGCTAAAAGTATTCCCGGAGTC
TATGATAGGCTTATAGAATTAGGGGTTAACTATCAAGAGTTAGAAAATAGTCTAAAATTAATAGCATATCAACGTTTAAT
TGGAGGAGGAAGCCTAATTGACTTTGAGACAAGTAACTTTAAAAAACACTCATCAGACAAGTGGAATAGACAAGTGGATA
TCTTGGCTGAAGAAGGATATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAAACAACGGAAAGTAGT
CCAACTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

68.81

96.285

0.663
  comYA Streptococcus mutans UA140

68.81

96.285

0.663
  comYA Streptococcus gordonii str. Challis substr. CH1

63.323

98.762

0.625
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

62.379

96.285

0.601
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

61.415

96.285

0.591
  comGA/cglA/cilD Streptococcus pneumoniae D39

61.415

96.285

0.591
  comGA/cglA/cilD Streptococcus pneumoniae R6

61.415

96.285

0.591
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

61.415

96.285

0.591
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.613

95.975

0.591
  comGA Lactococcus lactis subsp. cremoris KW2

50.621

99.69

0.505