Detailed information    

insolico Bioinformatically predicted

Overview

Name   cinA   Type   Machinery gene
Locus tag   POF65_RS10320 Genome accession   NZ_CP116706
Coordinates   2025342..2026601 (-) Length   419 a.a.
NCBI ID   WP_001200975.1    Uniprot ID   A0AAV3JP75
Organism   Streptococcus agalactiae strain A909 sCas9     
Function   facilitate localization of RecA (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2020342..2031601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF65_RS10285 (POF65_10285) - 2020493..2022034 (-) 1542 WP_000230040.1 membrane protein -
  POF65_RS10290 (POF65_10290) - 2022080..2022202 (-) 123 WP_001285708.1 hypothetical protein -
  POF65_RS10295 (POF65_10295) - 2022285..2022602 (-) 318 WP_000940931.1 DUF1292 domain-containing protein -
  POF65_RS10300 (POF65_10300) ruvX 2022628..2023047 (-) 420 WP_001222109.1 Holliday junction resolvase RuvX -
  POF65_RS10305 (POF65_10305) - 2023047..2023313 (-) 267 WP_000507056.1 IreB family regulatory phosphoprotein -
  POF65_RS10310 (POF65_10310) spx 2023515..2023913 (-) 399 WP_000591161.1 transcriptional regulator Spx -
  POF65_RS10315 (POF65_10315) recA 2024129..2025268 (-) 1140 WP_001085741.1 recombinase RecA Machinery gene
  POF65_RS10320 (POF65_10320) cinA 2025342..2026601 (-) 1260 WP_001200975.1 competence/damage-inducible protein A Machinery gene
  POF65_RS10325 (POF65_10325) - 2026690..2027241 (-) 552 WP_000817341.1 DNA-3-methyladenine glycosylase I -
  POF65_RS10330 (POF65_10330) ruvA 2027264..2027854 (-) 591 WP_000272490.1 Holliday junction branch migration protein RuvA -
  POF65_RS10335 (POF65_10335) - 2027856..2029088 (-) 1233 WP_000657472.1 MFS transporter -
  POF65_RS10340 (POF65_10340) mutL 2029120..2031093 (-) 1974 WP_000034614.1 DNA mismatch repair endonuclease MutL -
  POF65_RS10345 (POF65_10345) - 2031319..2031522 (+) 204 WP_000191849.1 cold shock domain-containing protein -

Sequence

Protein


Download         Length: 419 a.a.        Molecular weight: 45454.24 Da        Isoelectric Point: 5.5601

>NTDB_id=779234 POF65_RS10320 WP_001200975.1 2025342..2026601(-) (cinA) [Streptococcus agalactiae strain A909 sCas9]
MRAEIIAVGTEILTGQIVNTNAQFLSEKMAELGIDIYFQTAVGDNEERLLSLLDIASQRSQLVILCGGLGPTEDDLTKQT
LAKFLGKSLTVDLLASQKLDRFFASRPQFARTPNNERQAQLVEGSIPLQNLTGLAVGGIVTSKGVQYMVLPGPPSELNPM
VMEQVVPILSNNGTKLYSRVLRFFGIGESQLVTILEDIIKNQTDPTIAPYAKVGEVTLRLSTKAENQDEADFKLDSLEKE
ILALKTLDNRKLKDLLYGYGDNNSMARTVLELLKVQNKTITAAESLTAGLFQSQLAEFSGASQVFNGGFTTYSMEAKSQL
LGIPKKKLQEYGVISHFTAEAMAQQARQLLKADFGIGLTGVAGPDELEGYPAGTVFIGIATPEGVSSIKVSIGGKSRSDV
RHISTLHAFDLVRRALLKI

Nucleotide


Download         Length: 1260 bp        

>NTDB_id=779234 POF65_RS10320 WP_001200975.1 2025342..2026601(-) (cinA) [Streptococcus agalactiae strain A909 sCas9]
GTGAGGGCTGAAATTATCGCTGTAGGAACAGAAATTTTAACAGGACAGATCGTTAATACAAACGCACAATTTTTGTCGGA
GAAAATGGCAGAATTGGGAATTGATATTTATTTCCAGACAGCTGTAGGAGACAATGAGGAACGTTTATTATCGCTTTTAG
ATATAGCTAGTCAGCGCAGTCAATTGGTTATCCTATGTGGTGGACTTGGACCTACAGAGGATGATTTAACGAAACAAACT
TTGGCTAAATTTTTAGGTAAATCATTAACAGTGGATTTATTAGCTAGTCAAAAATTGGATCGTTTTTTTGCAAGTCGTCC
TCAGTTTGCTAGAACGCCAAATAATGAACGACAAGCTCAATTGGTTGAAGGCTCAATACCTCTACAAAACCTTACTGGTC
TAGCTGTTGGTGGTATCGTTACATCTAAGGGAGTCCAATATATGGTTTTACCAGGTCCGCCTAGTGAATTAAATCCAATG
GTTATGGAGCAGGTGGTACCTATTTTGTCAAATAATGGTACAAAATTATATTCACGTGTATTGAGATTCTTTGGTATCGG
TGAGAGTCAGTTAGTCACAATCTTAGAAGACATAATTAAGAATCAGACAGATCCAACTATAGCACCATATGCAAAGGTTG
GTGAGGTAACTTTAAGGTTATCAACGAAAGCTGAAAATCAGGATGAAGCCGACTTTAAATTAGATAGCTTAGAAAAAGAA
ATACTTGCTCTTAAGACATTAGATAACCGTAAATTAAAAGATTTATTATATGGTTATGGTGATAATAATAGTATGGCACG
TACTGTTTTAGAATTATTGAAAGTACAAAATAAAACGATTACTGCCGCAGAGAGCTTAACAGCAGGGTTATTTCAATCTC
AGTTAGCAGAATTTTCTGGTGCTTCGCAAGTTTTTAATGGTGGTTTTACGACCTATAGTATGGAAGCAAAATCACAACTT
CTCGGCATCCCTAAGAAAAAATTGCAAGAATATGGAGTTATCAGTCATTTTACAGCTGAGGCAATGGCTCAGCAAGCTCG
CCAACTTTTAAAAGCAGATTTTGGTATCGGTTTAACGGGAGTTGCTGGTCCTGATGAGTTGGAAGGATACCCTGCAGGTA
CAGTTTTTATTGGGATTGCAACCCCTGAAGGCGTTTCTTCTATTAAAGTATCGATTGGAGGGAAGAGCCGCTCAGATGTC
CGTCATATCAGTACTTTACATGCTTTTGACCTGGTGCGTAGGGCTTTATTAAAGATATAA

Domains

Predicted by InterproScan.

Damage-inducible protein CinA KH domain (CinA_KH)

(178-241)

Probable molybdopterin binding domain (MoCF_biosynth)

(5-170)

Competence-damaged protein (CinA)

(263-415)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cinA Streptococcus mitis SK321

71.223

99.523

0.709
  cinA Streptococcus mitis NCTC 12261

70.983

99.523

0.706
  cinA Streptococcus pneumoniae TIGR4

69.784

99.523

0.695
  cinA Streptococcus pneumoniae Rx1

69.784

99.523

0.695
  cinA Streptococcus pneumoniae R6

69.784

99.523

0.695
  cinA Streptococcus pneumoniae D39

69.544

99.523

0.692
  cinA Streptococcus mutans UA159

67.866

99.523

0.675
  cinA Streptococcus suis isolate S10

54.567

99.284

0.542
  cinA Bacillus subtilis subsp. subtilis str. 168

43.541

99.761

0.434