Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PMC74_RS24795 Genome accession   NZ_CP116669
Coordinates   5538133..5539401 (-) Length   422 a.a.
NCBI ID   WP_084213304.1    Uniprot ID   -
Organism   Pseudomonas capeferrum strain TDA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5533133..5544401
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMC74_RS24780 (PMC74_24780) - 5534775..5536400 (-) 1626 WP_033702003.1 SPOR domain-containing protein -
  PMC74_RS24785 (PMC74_24785) aroB 5536416..5537513 (-) 1098 WP_047582202.1 3-dehydroquinate synthase -
  PMC74_RS24790 (PMC74_24790) aroK 5537610..5538128 (-) 519 WP_033702005.1 shikimate kinase AroK -
  PMC74_RS24795 (PMC74_24795) pilQ 5538133..5539401 (-) 1269 WP_084213304.1 type IV pilus secretin PilQ Machinery gene
  PMC74_RS24800 (PMC74_24800) - 5539325..5540323 (-) 999 WP_047582198.1 pilus assembly protein PilP -
  PMC74_RS24805 (PMC74_24805) - 5540338..5540874 (-) 537 WP_033702008.1 PilN domain-containing protein -
  PMC74_RS24810 (PMC74_24810) pilM 5540871..5541734 (-) 864 WP_047582196.1 type IV pilus biogenesis protein PilM -
  PMC74_RS24815 (PMC74_24815) - 5541916..5544366 (+) 2451 WP_371856397.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 46054.91 Da        Isoelectric Point: 5.0904

>NTDB_id=778979 PMC74_RS24795 WP_084213304.1 5538133..5539401(-) (pilQ) [Pseudomonas capeferrum strain TDA1]
MKKLWKWMVAWVLAIPLASATPYRGEPLSLNFQDVQVRAVLQVLADYAGVNLVASDAVQGNITLRLQDVPWDQALDLVLR
SKGLARREEGNVLLVAPAAELAGQSLDARMGQVLDAQLQPLQRELMPIHHAEASALAQLLLATLADDGILTGRGSLSVDA
RTNTLVAYQPADRLDELRQLVAQLDVPVRQVAIEARIVEASVDYEKSLGVRWGGPLYDERVRPGKELFVDLGVERAGSSV
GLGVLRGGILLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSKSGATSVSFREASLSLEVTPQITPDN
KVIMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTTQNNVVDKVPFFGDLPYVGRLFRRDVLQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=778979 PMC74_RS24795 WP_084213304.1 5538133..5539401(-) (pilQ) [Pseudomonas capeferrum strain TDA1]
ATGAAGAAGCTCTGGAAATGGATGGTGGCGTGGGTCTTGGCGATCCCGCTAGCGTCGGCAACGCCCTACCGGGGTGAGCC
GCTGTCGCTGAACTTTCAGGATGTTCAGGTGCGCGCGGTGTTGCAGGTGCTGGCGGACTACGCCGGTGTGAACCTGGTCG
CCAGCGACGCGGTGCAGGGCAATATCACCCTGCGGCTTCAGGATGTGCCCTGGGACCAGGCGCTTGATCTGGTGCTGCGC
AGCAAGGGCCTGGCCCGTCGCGAAGAGGGTAATGTGCTGCTGGTGGCGCCTGCGGCCGAGCTTGCCGGGCAGTCGCTCGA
CGCCCGCATGGGCCAGGTGCTGGATGCCCAGTTGCAACCCCTGCAGCGTGAGCTGATGCCGATCCATCACGCCGAAGCTT
CAGCGCTCGCGCAACTGCTGCTGGCGACACTGGCGGACGACGGCATCCTGACCGGGCGAGGCAGTTTGAGCGTCGATGCG
CGCACCAACACCCTGGTGGCCTACCAACCCGCCGATCGCCTGGACGAGCTACGCCAACTGGTGGCGCAGCTGGATGTGCC
GGTGCGCCAGGTGGCGATCGAGGCGCGTATTGTCGAGGCCAGCGTCGATTACGAAAAGAGCCTAGGGGTGCGTTGGGGTG
GGCCTTTGTACGATGAGCGCGTGCGGCCAGGCAAGGAGCTGTTCGTCGACCTTGGCGTGGAGCGTGCCGGCTCCAGTGTG
GGCCTGGGTGTGCTGCGCGGGGGCATCTTGCTCGACCTTGAACTCAGTGCCATGGAGAAGAGCGGCAATGGCGAAATCAT
CTCGCAGCCGAAGGTGGTCACGGCCGACAAGGAAACCGCCAGGATCCTCAAGGGCACTGAAGTGCCTTACCAGGAAACCA
GCAAGAGCGGCGCCACTTCGGTGTCGTTTCGCGAGGCTTCGCTGTCGCTCGAAGTCACCCCGCAAATCACCCCGGACAAC
AAGGTCATCATGACCGTCAGGGTGACCAAGGACGAGCCTGACTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAA
GAACGAGGTCAATGCCAAGGTTCGCGTGGCCGACGGCGAGACGATCGTGATCGGTGGCGTCTACTCGACCACGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGTATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)

Bacterial type II and III secretion system protein (Secretin)

(257-413)

Secretin and TonB N terminus short domain (STN)

(51-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.633

100

0.616
  pilQ Acinetobacter baumannii D1279779

42.424

100

0.464
  pilQ Acinetobacter baumannii strain A118

42.424

100

0.464
  comQ Acinetobacter baylyi ADP1

42.667

100

0.455
  pilQ Legionella pneumophila strain Lp02

42.823

99.052

0.424
  pilQ Legionella pneumophila strain ERS1305867

42.823

99.052

0.424
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.548

98.578

0.419
  pilQ Vibrio cholerae strain A1552

42.548

98.578

0.419
  pilQ Vibrio campbellii strain DS40M4

40.046

100

0.41