Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PH545_RS01410 Genome accession   NZ_CP116414
Coordinates   264462..266147 (-) Length   561 a.a.
NCBI ID   WP_017189132.1    Uniprot ID   -
Organism   Vibrio harveyi strain K2014767     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 259462..271147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PH545_RS01380 (PH545_01380) rplS 259704..260057 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  PH545_RS01385 (PH545_01385) yacG 260623..260823 (-) 201 WP_026000212.1 DNA gyrase inhibitor YacG -
  PH545_RS01390 (PH545_01390) zapD 260880..261620 (-) 741 WP_038897986.1 cell division protein ZapD -
  PH545_RS01395 (PH545_01395) coaE 261648..262262 (-) 615 WP_289880840.1 dephospho-CoA kinase -
  PH545_RS01400 (PH545_01400) pilD 262263..263132 (-) 870 WP_045488712.1 A24 family peptidase Machinery gene
  PH545_RS01405 (PH545_01405) pilC 263205..264428 (-) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  PH545_RS01410 (PH545_01410) pilB 264462..266147 (-) 1686 WP_017189132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PH545_RS01415 (PH545_01415) pilA 266151..266615 (-) 465 WP_005446258.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  PH545_RS01420 (PH545_01420) nadC 266880..267767 (-) 888 WP_053052948.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PH545_RS01425 (PH545_01425) ampD 267860..268411 (+) 552 WP_154203416.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PH545_RS01430 (PH545_01430) pdhR 268816..269583 (+) 768 WP_005446261.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62014.83 Da        Isoelectric Point: 5.2849

>NTDB_id=777277 PH545_RS01410 WP_017189132.1 264462..266147(-) (pilB) [Vibrio harveyi strain K2014767]
MLTNLSTVLRQAGILTFSQEESLLEQAKASGISMPEALLSSGFFQSHELTEHLSSIFGLSCTSLSQYEYSSLCQKLGLRD
LITRHNALPLNRTSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFKELTAAIRRLYGRALGQEKSGLKEINQDELAG
LVDIGEDEIENIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYENMYRVRLRCDGILIEIQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSAALDINKLGYSDHQKQLYLEALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKTEINISTAEDPVEINLPGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCPHCRLPQEPNSQLQHIGITSTED
IFQANPDGCNECTQGYSGRTGIYEVMKFDESLSNALIKGASVHELEKLAIANGMQTLQMSGIEKLKQGVTSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=777277 PH545_RS01410 WP_017189132.1 264462..266147(-) (pilB) [Vibrio harveyi strain K2014767]
ATGTTAACCAACCTCTCAACAGTTCTTCGTCAAGCGGGAATACTGACCTTTTCCCAAGAAGAATCGTTACTTGAGCAAGC
TAAAGCTTCTGGTATTTCGATGCCGGAGGCTTTACTCAGTTCTGGCTTTTTCCAATCACATGAATTGACTGAGCACCTTA
GCTCCATCTTTGGGCTTTCCTGCACATCACTCAGTCAGTATGAGTACTCGTCGCTGTGCCAAAAGCTTGGCTTACGCGAT
CTGATCACTCGACATAATGCCTTGCCACTCAATCGCACATCTTCGACATTAATACTCGCGGTTGCAGACCCAACTAACTT
ACAAGCGGAAGATGACTTTCGTTTTGCTACCGGTTTACAAGTCGAGCTAGTTCTGGCGGACTTTAAAGAACTCACCGCAG
CCATTCGTCGTTTATATGGTCGTGCACTCGGTCAAGAAAAGTCCGGTCTTAAAGAAATCAACCAAGATGAACTGGCAGGT
CTCGTTGATATTGGTGAGGACGAAATTGAGAACATTGAAGATCTCAGCCAAGATGAATCGCCGGTTAGTCGTTATATTAA
CCAAATTCTGCTCGATGCCGTTCGTAAAGGGGCTTCTGATATTCACTTCGAGCCCTATGAAAACATGTATCGAGTGCGTT
TGCGTTGCGATGGCATCTTGATCGAAATTCAACAGCCTCCGAGCCATTTAAGCCGTCGCCTCTCAGCCCGAATAAAGATC
CTATCGAAGCTTGATATCGCTGAGCGCCGTTTACCACAGGATGGTCGCATCAAGCTCAAATTAAACCAAGACACCGCGAT
TGACATGCGAGTCTCCACGCTGCCAACTCTGTTTGGGGAGAAAATCGTACTGCGTTTGCTCGACAGCAGTTCTGCAGCAT
TAGATATCAATAAACTCGGATACAGCGATCATCAAAAGCAATTGTATTTAGAAGCACTGCGCCGCCCACAAGGCATGATC
CTAATGACAGGACCAACGGGCAGCGGCAAAACGGTTTCTCTTTACACTGGTTTGAGCATTCTCAATAAAACCGAGATCAA
CATTTCCACCGCTGAAGACCCCGTTGAGATCAACTTACCCGGCATTAACCAAGTTCAAGTTCAACCTAAGATCGGCTTTG
GCTTTGCAGAAGCGCTTCGCTCTTTCTTGCGTCAAGATCCCGATGTAGTCATGGTGGGTGAGATCCGAGATCTCGACACG
GCAGAAATCGCGATCAAAGCGTCACAAACTGGGCACTTAGTCCTTTCTACCCTGCATACCAACTCCGCCGCCGAGACGGT
TATTCGCTTATCAAATATGGGGGTAGAAAGCTTTAACCTCGCATCCTCTCTTAGTTTGATCATCGCTCAGCGTTTAGCTC
GAAAACTTTGTCCACATTGCAGACTACCTCAAGAACCAAACAGCCAACTTCAACATATTGGAATTACCAGCACTGAAGAT
ATCTTCCAAGCGAACCCTGACGGTTGTAACGAATGCACCCAAGGATACTCTGGTCGCACAGGCATTTACGAAGTGATGAA
GTTTGATGAATCCCTGTCAAATGCGCTCATCAAAGGTGCTTCAGTGCATGAATTAGAAAAGCTAGCGATTGCCAATGGTA
TGCAGACTCTACAAATGTCCGGCATTGAAAAGCTCAAACAAGGCGTCACCAGCTTTAGTGAGCTGCAACGCGTTCTCTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

96.435

100

0.964
  pilB Vibrio parahaemolyticus RIMD 2210633

89.483

100

0.895
  pilB Vibrio cholerae strain A1552

73.132

100

0.733
  pilB Acinetobacter baylyi ADP1

48.294

99.287

0.479
  pilB Acinetobacter baumannii D1279779

51.838

92.157

0.478
  pilB Legionella pneumophila strain ERS1305867

48.155

96.613

0.465
  pilF Neisseria gonorrhoeae MS11

43.06

100

0.431
  pilB/pilB1 Synechocystis sp. PCC 6803

37.037

100

0.392
  pilF Thermus thermophilus HB27

36.477

100

0.365
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.738

99.465

0.365