Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PH545_RS01415 Genome accession   NZ_CP116414
Coordinates   266151..266615 (-) Length   154 a.a.
NCBI ID   WP_005446258.1    Uniprot ID   -
Organism   Vibrio harveyi strain K2014767     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 261151..271615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PH545_RS01395 (PH545_01395) coaE 261648..262262 (-) 615 WP_289880840.1 dephospho-CoA kinase -
  PH545_RS01400 (PH545_01400) pilD 262263..263132 (-) 870 WP_045488712.1 A24 family peptidase Machinery gene
  PH545_RS01405 (PH545_01405) pilC 263205..264428 (-) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  PH545_RS01410 (PH545_01410) pilB 264462..266147 (-) 1686 WP_017189132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PH545_RS01415 (PH545_01415) pilA 266151..266615 (-) 465 WP_005446258.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  PH545_RS01420 (PH545_01420) nadC 266880..267767 (-) 888 WP_053052948.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PH545_RS01425 (PH545_01425) ampD 267860..268411 (+) 552 WP_154203416.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PH545_RS01430 (PH545_01430) pdhR 268816..269583 (+) 768 WP_005446261.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16024.27 Da        Isoelectric Point: 5.8347

>NTDB_id=777278 PH545_RS01415 WP_005446258.1 266151..266615(-) (pilA) [Vibrio harveyi strain K2014767]
MKTNKQKKQQGFTLIELMIVVAIIGILAAFAVPAYQNYTKKATLAEFPKAAAAMKLAVELCAHENASDAATFKTNCLSGS
NGVPSKIADLNDISIEAVAGSSGVNVIAKAIAVKGPIAANEQYTMASNYTASGIEWTGSCQDSGGNAQTDYCPE

Nucleotide


Download         Length: 465 bp        

>NTDB_id=777278 PH545_RS01415 WP_005446258.1 266151..266615(-) (pilA) [Vibrio harveyi strain K2014767]
ATGAAAACGAATAAACAGAAGAAGCAGCAGGGTTTTACTCTAATTGAATTGATGATTGTGGTTGCAATTATTGGTATTTT
GGCTGCATTTGCAGTACCTGCATATCAAAACTATACAAAAAAAGCGACATTAGCGGAGTTCCCAAAAGCAGCAGCAGCTA
TGAAGTTAGCAGTTGAATTGTGCGCGCACGAAAATGCTTCAGATGCAGCGACCTTTAAAACAAACTGCCTTTCTGGTAGC
AATGGAGTGCCTTCAAAGATTGCTGATTTAAACGATATATCTATTGAAGCTGTCGCTGGTTCCTCTGGCGTAAATGTCAT
TGCAAAAGCAATTGCAGTGAAAGGACCTATCGCAGCTAATGAACAATACACAATGGCATCAAACTACACTGCAAGTGGTA
TTGAATGGACTGGCTCTTGTCAGGATTCTGGGGGGAACGCTCAGACTGATTACTGTCCTGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

56.774

100

0.571
  pilA Haemophilus influenzae 86-028NP

41.958

92.857

0.39
  pilA2 Legionella pneumophila strain ERS1305867

39.865

96.104

0.383
  pilA2 Legionella pneumophila str. Paris

39.865

96.104

0.383