Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PJ259_RS11715 Genome accession   NZ_CP116340
Coordinates   2530505..2532199 (-) Length   564 a.a.
NCBI ID   WP_271652932.1    Uniprot ID   A0AAX3KS27
Organism   Pseudomonas sp. JBR1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2525505..2537199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PJ259_RS11690 (PJ259_11690) - 2526892..2527389 (+) 498 WP_271652928.1 hypothetical protein -
  PJ259_RS11695 (PJ259_11695) yacG 2527403..2527600 (-) 198 WP_007160931.1 DNA gyrase inhibitor YacG -
  PJ259_RS11700 (PJ259_11700) coaE 2527597..2528208 (-) 612 WP_271652929.1 dephospho-CoA kinase -
  PJ259_RS11705 (PJ259_11705) pilD 2528205..2529077 (-) 873 WP_271652930.1 A24 family peptidase Machinery gene
  PJ259_RS11710 (PJ259_11710) pilC 2529077..2530294 (-) 1218 WP_271652931.1 type II secretion system F family protein Machinery gene
  PJ259_RS11715 (PJ259_11715) pilB 2530505..2532199 (-) 1695 WP_271652932.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PJ259_RS11720 (PJ259_11720) pilA 2532412..2532849 (+) 438 WP_271652933.1 pilin Machinery gene
  PJ259_RS11725 (PJ259_11725) - 2532892..2534346 (+) 1455 WP_271652934.1 O-antigen ligase family protein -
  PJ259_RS11730 (PJ259_11730) - 2534664..2535662 (-) 999 WP_271652935.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62618.03 Da        Isoelectric Point: 5.6097

>NTDB_id=776680 PJ259_RS11715 WP_271652932.1 2530505..2532199(-) (pilB) [Pseudomonas sp. JBR1]
MSETVMLSGLARQLVDAQLLAEIAAQQAVIKAKQSKVPLVTYLVQQRLVQSRPLAELAAQEFGVPFLDLSAIDKEQLPKD
IVNEKLMQQHHFLPLSRRGNKLFIALSDPTNQQAINDVSFGSKLAVEVVLVEDDKLHQAIERYLDTGTGGLELSDLDLDL
ETEGGERPQEAVAGTDADDAPVVKFVNKMLLDAIKKGSSDLHFEPYEKMYRVRFRTDGVLHEAARPPVQLVSRISARLKV
MSNMDISERRKPQDGRVKLKVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEDMQKELYLQALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTEDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNLATSVNLIIAQRLARKLCNKCKRIHEVPREALLAEGFREEE
IGSFTLYQPIGCEDCNAGYRGRVGIYEVVKVTPALQQMIMEEGNSIEIAARMRMEGFNDLRRSGLVKAMQGITSLEEINR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=776680 PJ259_RS11715 WP_271652932.1 2530505..2532199(-) (pilB) [Pseudomonas sp. JBR1]
ATGAGCGAGACGGTAATGCTGTCGGGGCTTGCGCGGCAACTGGTGGATGCGCAGTTACTAGCGGAGATAGCGGCCCAGCA
AGCAGTGATCAAGGCCAAGCAGAGCAAGGTTCCGCTGGTGACCTATCTCGTTCAGCAGCGGCTGGTGCAGAGCCGACCTT
TGGCTGAGCTAGCAGCCCAAGAATTTGGCGTGCCCTTTCTCGATCTGTCGGCCATTGACAAAGAGCAGTTACCCAAGGAC
ATCGTCAACGAGAAGCTGATGCAACAGCATCACTTCCTGCCTCTGAGCAGAAGAGGTAATAAGCTCTTCATTGCACTCTC
TGACCCGACCAATCAGCAAGCCATCAACGATGTATCCTTTGGCAGCAAATTGGCGGTTGAGGTGGTGCTGGTCGAGGACG
ACAAGCTGCACCAAGCCATCGAGCGGTATCTGGATACGGGAACCGGCGGTTTGGAGTTGTCCGATCTTGACCTTGATCTG
GAAACCGAAGGAGGAGAGCGACCGCAGGAGGCGGTAGCTGGTACTGACGCTGACGATGCGCCCGTGGTGAAGTTCGTCAA
CAAGATGCTGCTCGACGCCATCAAGAAGGGCTCGTCCGACCTGCACTTCGAGCCCTATGAAAAGATGTACCGGGTCCGTT
TTCGTACCGATGGCGTTCTGCACGAGGCGGCGCGACCGCCGGTGCAACTGGTGTCGCGGATCTCGGCGCGGCTCAAGGTG
ATGTCCAATATGGATATCTCCGAGCGACGCAAGCCCCAGGATGGCCGGGTCAAGCTCAAGGTATCCAAGACTAAGTCCAT
CGACTTCCGGGTCAATACCTTGCCCACCCTTTGGGGTGAGAAGATCGTGATGCGGATTCTGGATTCCTCCAGCGCTCAGA
TGGGGATCGATGCCCTGGGCTACGAGGATATGCAGAAGGAACTTTACCTGCAGGCGCTCAAGCAGCCGCAGGGGATGATC
CTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTCTCTCTTTATACCGGCCTAAATATTCTCAATACCGAGGACATCAA
TATTTCCACTGCTGAGGATCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGTCAAGGGCTCG
ATTTCTCCCAAGCGCTACGGGCCTTTCTGCGTCAAGATCCCGACGTGATCATGGTCGGTGAGATTCGTGACCTGGAAACG
GCGGAGATCGCCATCAAGGCGGCCCAGACCGGCCACATGGTGATGTCGACCTTGCACACCAACAGCGCGGCGGAAACCCT
GACCCGTCTGCGGAACATGGGGGTGCCCGCCTTCAATCTGGCGACTTCGGTCAACCTCATCATCGCCCAGCGCTTGGCGC
GCAAGCTGTGCAACAAGTGCAAGAGAATCCATGAGGTGCCGCGTGAGGCATTGCTGGCTGAAGGCTTCCGCGAGGAAGAG
ATCGGCAGTTTTACCCTTTATCAGCCTATCGGCTGCGAGGACTGCAATGCGGGTTATCGTGGACGCGTGGGTATCTATGA
GGTGGTGAAGGTCACCCCGGCGCTACAGCAGATGATCATGGAGGAGGGCAACTCCATCGAGATCGCGGCGCGGATGCGGA
TGGAAGGTTTCAATGATCTGCGGCGGTCGGGACTGGTGAAGGCTATGCAGGGGATTACCAGTCTGGAAGAGATCAATCGG
GTGACCAAGGACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.715

99.468

0.594
  pilB Acinetobacter baumannii D1279779

59.358

99.468

0.59
  pilB Legionella pneumophila strain ERS1305867

53.793

100

0.553
  pilB Vibrio cholerae strain A1552

50.177

100

0.502
  pilB Vibrio campbellii strain DS40M4

50.089

99.823

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.468

0.496
  pilF Neisseria gonorrhoeae MS11

48.845

99.823

0.488
  pilF Thermus thermophilus HB27

36.861

100

0.371
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.048

93.085

0.363