Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H7R46_RS16950 Genome accession   NZ_AP022302
Coordinates   3569952..3571664 (+) Length   570 a.a.
NCBI ID   WP_032012470.1    Uniprot ID   -
Organism   Acinetobacter sp. BEC1-S18-ESBL-01     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3564952..3576664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R46_RS16915 (BE1S18E01_32890) nusA 3565840..3567324 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  H7R46_RS16920 (BE1S18E01_32900) rimP 3567362..3567886 (-) 525 WP_002114922.1 ribosome maturation factor RimP -
  H7R46_RS16940 (BE1S18E01_32910) secG 3568524..3568853 (-) 330 WP_002115012.1 preprotein translocase subunit SecG -
  H7R46_RS16945 (BE1S18E01_32920) tpiA 3568866..3569660 (-) 795 WP_032012469.1 triose-phosphate isomerase -
  H7R46_RS16950 (BE1S18E01_32930) pilB 3569952..3571664 (+) 1713 WP_032012470.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H7R46_RS16955 (BE1S18E01_32940) pilC 3571691..3572917 (+) 1227 WP_042783374.1 type II secretion system F family protein Machinery gene
  H7R46_RS16960 (BE1S18E01_32950) pilD 3572917..3573777 (+) 861 WP_032012472.1 prepilin peptidase Machinery gene
  H7R46_RS16965 (BE1S18E01_32960) coaE 3573779..3574375 (+) 597 WP_032012473.1 dephospho-CoA kinase -
  H7R46_RS16970 (BE1S18E01_32970) - 3574372..3575286 (-) 915 WP_042783373.1 DMT family transporter -
  H7R46_RS16975 (BE1S18E01_32980) rlmB 3575322..3576071 (-) 750 WP_005079348.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  H7R46_RS16980 (BE1S18E01_32990) - 3576174..3576500 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63513.90 Da        Isoelectric Point: 5.0271

>NTDB_id=77656 H7R46_RS16950 WP_032012470.1 3569952..3571664(+) (pilB) [Acinetobacter sp. BEC1-S18-ESBL-01]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVNLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLVLKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILAQQFTEESSFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIASPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKIPADIPKQSLLEMGF
NDQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=77656 H7R46_RS16950 WP_032012470.1 3569952..3571664(+) (pilB) [Acinetobacter sp. BEC1-S18-ESBL-01]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAGAGAGCTAAACGGGATACGGTTGCGTACCTGATTGAAGAGGTTAATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACAAGCCAAATT
CCCAAAGATTTGGTCGATCAGAAACTAGTTTTAAAACATCGGGTCTTACCTCTCATACAAAGAGGACAGATTTTATATGT
CGCAACCAGCAACCCGAGTAATATTGAAGCAATTGATGCCATTCGCTTTAATAGCAAGCTCATGGTTGAACCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGGCGCAGCAATTTACCGAAGAAAGCAGCTTCGAATTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCTCCACCCTCACAAGAGGATGAGGAAGAAACACCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCGATTCGTATGGGGGCTTCAGACTTACATTTTGAACCATACGAGAAATCAT
ATCGGGTCCGTTATCGAGTCGACGGCGTGTTACGTCAGATTGCAAGTCCACCTTTGCAATTGGCTAACCGTTTGGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAGAAACGAGTGCCTCAAGATGGTCGTATCAAGCTTAAGTTATCAAA
GACTAAAGCAATCGATTTTCGTGTGAACTCACTTCCAACCTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CTAGCGCAATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTTTAATTACAGGTCCTACTGGTTCTGGTAAAACGGTATCTTTATATACAGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACCGCCGAAGATCCAGTTGAAATCAACCTTGAAGGCATTAATCAGGTTAACGTTAACCCAA
AAGTAGGTCTTACTTTTTCGGCCGCACTGAAATCATTTTTACGTCAGGACCCCGACATTATTATGGTCGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCACAAACAGGTCACATGGTCATGTCCACACTGCATACCAATAGTGC
ACCAGAAACACTTACTCGCCTACGTAATATGGGTGTTCCATCCTTTAATATCGCAACATCAGTCAACTTGGTTATTGCAC
AGCGTTTAGCACGTCGGCTATGCCCACAGTGTAAAATACCTGCTGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
AACGACCAAGATTTAACCCATCCCGATTTTCAAGTTTTTCAACCTGTAGGTTGTACTGAGTGTCGTGAAGGCTACAAAGG
TCGAGTAGGTATTTATGAGGTGATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGGTTAAAGAAAGTCATGCAAGGTGTAACTTCC
TTACAAGAAGTTAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.561

100

0.946
  pilB Acinetobacter baylyi ADP1

77.895

100

0.779
  pilB Legionella pneumophila strain ERS1305867

51.323

99.474

0.511
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

51.718

91.93

0.475
  pilB Vibrio campbellii strain DS40M4

50.766

91.579

0.465
  pilB Vibrio cholerae strain A1552

50.289

91.053

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

35.342

100

0.381
  pilF Thermus thermophilus HB27

36.655

98.596

0.361

Multiple sequence alignment