Detailed information NTG0077658

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: H7R46_RS16960
• Genome accession: NZ_AP022302
• Coordinates: 3572917..3573777 (+)
• Length: 286 a.a.
• NCBI ID: WP_032012472.1
• Uniprot ID: A0A022J1F4
• Organism: Acinetobacter sp. BEC1-S18-ESBL-01
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3567917..3578777

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H7R46_RS16940 (BE1S18E01_32910)	secG	3568524..3568853 (-)	330	WP_002115012.1	preprotein translocase subunit SecG	-
H7R46_RS16945 (BE1S18E01_32920)	tpiA	3568866..3569660 (-)	795	WP_032012469.1	triose-phosphate isomerase	-
H7R46_RS16950 (BE1S18E01_32930)	pilB	3569952..3571664 (+)	1713	WP_032012470.1	type IV-A pilus assembly ATPase PilB	Machinery gene
H7R46_RS16955 (BE1S18E01_32940)	pilC	3571691..3572917 (+)	1227	WP_042783374.1	type II secretion system F family protein	Machinery gene
H7R46_RS16960 (BE1S18E01_32950)	pilD	3572917..3573777 (+)	861	WP_032012472.1	prepilin peptidase	Machinery gene
H7R46_RS16965 (BE1S18E01_32960)	coaE	3573779..3574375 (+)	597	WP_032012473.1	dephospho-CoA kinase	-
H7R46_RS16970 (BE1S18E01_32970)	-	3574372..3575286 (-)	915	WP_042783373.1	DMT family transporter	-
H7R46_RS16975 (BE1S18E01_32980)	rlmB	3575322..3576071 (-)	750	WP_005079348.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
H7R46_RS16980 (BE1S18E01_32990)	-	3576174..3576500 (-)	327	WP_002115102.1	pyrimidine/purine nucleoside phosphorylase	-
H7R46_RS16985 (BE1S18E01_33000)	-	3576577..3577887 (-)	1311	WP_032012475.1	tetratricopeptide repeat protein	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 32087.56 Da
• Isoelectric Point: 6.4261

>NTDB_id=77658 H7R46_RS16960 WP_032012472.1 3572917..3573777(+) (pilD) [Acinetobacter sp. BEC1-S18-ESBL-01]
MQEIIAYFIQNLTALYIAVALLSLCIGSFLNVVIYRTPKMMEQDWQQECQILLNPELPIIEHEKLTLSKPASSCPACQQP
IRWYQNIPVISWLMLRGKCGDCQHPISIRYPAIELLTMLCSLVVVMVFGPTLQMLWGLVLTWVLIALTFIDFDTQLLPDR
FTLPLAALGLGINTFSIYTSPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
LGAIIGIILLKLRNDNQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG

Nucleotide

• Length: 861 bp

>NTDB_id=77658 H7R46_RS16960 WP_032012472.1 3572917..3573777(+) (pilD) [Acinetobacter sp. BEC1-S18-ESBL-01]
ATGCAAGAAATTATTGCTTATTTTATTCAAAACTTAACTGCACTTTATATTGCAGTCGCACTATTGAGCCTATGTATTGG
TAGCTTTTTGAATGTGGTAATTTATCGCACCCCCAAAATGATGGAACAAGATTGGCAGCAGGAATGTCAAATATTGCTTA
ATCCCGAACTACCCATCATTGAGCATGAAAAGCTGACATTAAGTAAACCTGCTTCATCATGTCCTGCATGTCAGCAACCG
ATCCGTTGGTATCAAAATATTCCAGTTATAAGCTGGTTAATGTTAAGAGGAAAATGTGGCGATTGCCAACATCCTATTAG
CATTCGTTATCCAGCGATTGAATTACTCACGATGCTATGTTCATTAGTAGTCGTCATGGTTTTTGGTCCAACCCTACAAA
TGCTTTGGGGGCTAGTGCTCACTTGGGTTTTGATTGCTTTAACATTTATAGACTTTGATACTCAGCTACTACCTGACCGT
TTCACCCTACCTTTGGCTGCTCTCGGCTTAGGTATTAATACCTTTAGTATTTACACCTCACCTAACTCAGCCATTTGGGG
ATATTTAATCGGCTTTTTATGTCTTTGGATTGTGTATTACTTATTTAAAGTGATTACAGGCAAAGAAGGTATGGGTTATG
GCGACTTTAAACTGCTTGCCGCATTAGGCGCGTGGATGGGGCCATTGATGCTGCCATTAATTGTGCTATTGTCATCTTTA
CTAGGTGCAATTATTGGCATCATTTTACTAAAATTACGTAATGATAATCAGCCCTTTGCGTTTGGGCCCTATATTGCCAT
TGCGGGTTGGGTTGCCTTTTTATGGGGTGACCAAATTATGAAAATTTATTTGGGAGGTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-128)

3D structure

• AlphaFold DB: A0A022J1F4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	95.804	100	0.958
pilD	Acinetobacter nosocomialis M2	95.455	100	0.955
pilD	Vibrio cholerae strain A1552	54.212	95.455	0.517
pilD	Vibrio campbellii strain DS40M4	53.676	95.105	0.51
pilD	Neisseria gonorrhoeae MS11	47.71	91.608	0.437