Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PGW89_RS06555 Genome accession   NZ_CP116040
Coordinates   1399346..1401061 (+) Length   571 a.a.
NCBI ID   WP_005083851.1    Uniprot ID   A0A380UBG2
Organism   Acinetobacter haemolyticus strain HW-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1394346..1406061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PGW89_RS06520 (PGW89_06520) nusA 1395154..1396638 (-) 1485 WP_004637688.1 transcription termination factor NusA -
  PGW89_RS06525 (PGW89_06525) rimP 1396676..1397200 (-) 525 WP_005083847.1 ribosome maturation factor RimP -
  PGW89_RS06545 (PGW89_06545) secG 1397836..1398165 (-) 330 WP_004637686.1 preprotein translocase subunit SecG -
  PGW89_RS06550 (PGW89_06550) tpiA 1398178..1398969 (-) 792 WP_005088004.1 triose-phosphate isomerase -
  PGW89_RS06555 (PGW89_06555) pilB 1399346..1401061 (+) 1716 WP_005083851.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PGW89_RS06560 (PGW89_06560) pilC 1401086..1402312 (+) 1227 WP_113997817.1 type II secretion system F family protein Machinery gene
  PGW89_RS06565 (PGW89_06565) pilD 1402312..1403172 (+) 861 WP_113997818.1 A24 family peptidase Machinery gene
  PGW89_RS06570 (PGW89_06570) coaE 1403174..1403779 (+) 606 WP_113997819.1 dephospho-CoA kinase -
  PGW89_RS06575 (PGW89_06575) - 1403776..1404687 (-) 912 WP_113997820.1 DMT family transporter -
  PGW89_RS06580 (PGW89_06580) rlmB 1404727..1405473 (-) 747 WP_004637678.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  PGW89_RS06585 (PGW89_06585) - 1405581..1405907 (-) 327 WP_004637677.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63323.54 Da        Isoelectric Point: 5.1480

>NTDB_id=775485 PGW89_RS06555 WP_005083851.1 1399346..1401061(+) (pilB) [Acinetobacter haemolyticus strain HW-2]
MSGLHTSPKFSGFIRRLVEDGHMSASTMQTAIDTAKKTQQDIVGYLIEHHRISQLTIAESISAEFGEPIFDLAVFDTALL
PRELADSKLIQKHRVAPLIQRGQILYVATSNPTNIDALDAMRFNTKLNIEPIIVEHSKLEKLIEQQFGDSGSFDFGDSED
FDLDIDVSQDSQKDESDETPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKTYRVRYRVDGVLRQIATPPLQMANRLA
SRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKALFMEALEK
PQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNTKTGLTFSAALKAFLRQDPDIIMVGEI
RDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSHCKAPIEIPKQSLLELG
FTEQDLADPELQIFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFNNLRRSGLKKVMQGIT
SLQEINRVTSE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=775485 PGW89_RS06555 WP_005083851.1 1399346..1401061(+) (pilB) [Acinetobacter haemolyticus strain HW-2]
ATGTCAGGATTACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGGTTAGTCGAAGATGGTCATATGTCTGCATCGAC
AATGCAAACTGCAATCGATACAGCAAAAAAAACACAACAAGATATTGTTGGATATTTAATTGAACATCATCGTATCTCTC
AACTGACCATTGCAGAGTCAATCTCGGCAGAGTTTGGCGAACCTATTTTTGATTTAGCTGTTTTTGATACTGCTTTATTA
CCTCGTGAGCTTGCTGACAGCAAATTAATTCAAAAGCATCGGGTTGCCCCTCTCATTCAACGCGGACAAATTCTTTATGT
CGCGACAAGCAATCCAACCAATATTGATGCGCTTGATGCGATGCGATTTAACACCAAACTCAATATTGAACCGATTATCG
TCGAACATAGTAAACTTGAAAAACTGATTGAACAGCAGTTTGGTGACTCTGGAAGTTTTGATTTTGGTGACAGTGAAGAC
TTTGACTTAGATATTGATGTCAGCCAAGATTCGCAAAAAGATGAAAGTGATGAAACGCCTCAAGGGGATGAATCACCAAT
TGTAAAATACATCAACAAGTTACTGATTGATGCCATTCGCATGGGTGCGTCTGATCTACATTTTGAACCCTATGAAAAAA
CCTATCGTGTTCGTTATCGCGTAGATGGTGTCTTAAGACAAATTGCGACGCCACCGTTGCAAATGGCAAATCGCTTAGCT
TCCCGTTTAAAAGTCATGTCTCAAATGGACATTTCTGAAAAACGAATGCCGCAAGATGGTCGTATTAAGCTCAAACTTTC
GAAAACCAAAGCGATTGACTTTCGTGTCAACTCACTGCCTACCCTATTTGGTGAAAAGCTAGTACTCCGTATTCTGGATC
CTTCCAGCGCGATGCTCGGAATTGAGGCACTCGGTTACGAGGATGAACAAAAAGCTCTATTTATGGAGGCGCTTGAAAAA
CCTCAAGGCATGCTGTTAATTACTGGCCCAACAGGTTCTGGTAAAACTGTGTCGTTATATACAGGTCTCAACATTCTAAA
TACTGAAGACACCAATATTTCTACTGCTGAAGACCCAGTGGAGATTAACTTAGAAGGAATCAATCAGGTTAACGTCAATA
CTAAGACAGGCCTTACCTTCTCTGCCGCGCTGAAAGCATTCTTACGTCAAGATCCAGACATTATCATGGTCGGTGAGATT
CGTGACCTAGAAACAGCAGAAATTGCAATCAAGGCTGCTCAAACGGGTCATATGGTGATGTCCACGCTACATACCAATAG
TGCACCTGAAACACTGACGCGTTTACGCAATATGGGCGTTCCCTCTTTCAATATCGCAACTTCGGTCAATCTTGTGATTG
CACAACGCTTAGCCCGTCGTTTATGCTCACACTGTAAAGCACCCATAGAGATCCCCAAACAAAGCTTATTAGAACTCGGC
TTTACTGAACAGGATTTAGCTGACCCTGAATTGCAAATCTTTCAACCAGTCGGCTGCTCAGAATGTCGTGAAGGCTATAA
AGGCCGTGTCGGTATCTATGAGGTCATGAAGGTGACGCCTGAGATCTCTAAAATTATTATGGAGGATGGCAATGCTTTAC
AAATCGCAGCAGCATCAGAAAAAGCAGGATTTAATAATTTAAGACGCTCAGGTTTAAAGAAAGTCATGCAAGGCATTACC
TCATTGCAAGAAATTAACCGCGTCACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A380UBG2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

82.137

100

0.821
  pilB Acinetobacter baylyi ADP1

77.583

100

0.776
  pilB Legionella pneumophila strain ERS1305867

53.659

93.345

0.501
  pilF Neisseria gonorrhoeae MS11

48.522

100

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

49.016

97.898

0.48
  pilB Vibrio cholerae strain A1552

48.644

96.848

0.471
  pilB Vibrio campbellii strain DS40M4

51.06

90.893

0.464
  pilF Thermus thermophilus HB27

37.478

98.599

0.37
  pilB/pilB1 Synechocystis sp. PCC 6803

33.659

100

0.363
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.795

92.995

0.361