Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   PF976_RS12965 Genome accession   NZ_CP116011
Coordinates   2493147..2493761 (-) Length   204 a.a.
NCBI ID   WP_014470701.1    Uniprot ID   A0AAP7N5G8
Organism   Bacillus amyloliquefaciens strain SRCM124317     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2488147..2498761
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF976_RS12935 (PF976_12935) rpsT 2488447..2488713 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  PF976_RS12940 (PF976_12940) holA 2488736..2489771 (-) 1036 Protein_2527 DNA polymerase III subunit delta -
  PF976_RS12945 (PF976_12945) - 2489811..2489965 (-) 155 Protein_2528 hypothetical protein -
  PF976_RS12950 (PF976_12950) - 2490006..2490140 (+) 135 WP_003152870.1 YqzM family protein -
  PF976_RS12955 (PF976_12955) - 2490159..2492511 (-) 2353 Protein_2530 DNA internalization-related competence protein ComEC/Rec2 -
  PF976_RS12960 (PF976_12960) - 2492512..2493081 (-) 570 WP_014470700.1 ComE operon protein 2 -
  PF976_RS12965 (PF976_12965) comEA 2493147..2493761 (-) 615 WP_014470701.1 helix-hairpin-helix domain-containing protein Machinery gene
  PF976_RS12970 (PF976_12970) comER 2493821..2494642 (+) 822 WP_014470702.1 late competence protein ComER -
  PF976_RS12975 (PF976_12975) - 2494711..2495448 (-) 738 WP_014470703.1 class I SAM-dependent DNA methyltransferase -
  PF976_RS12980 (PF976_12980) rsfS 2495445..2495801 (-) 357 WP_014470704.1 ribosome silencing factor -
  PF976_RS12985 (PF976_12985) yqeK 2495818..2496378 (-) 561 WP_147786059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  PF976_RS12990 (PF976_12990) - 2496368..2496937 (-) 570 WP_014470706.1 nicotinate-nucleotide adenylyltransferase -
  PF976_RS12995 (PF976_12995) yhbY 2496948..2497238 (-) 291 WP_014470707.1 ribosome assembly RNA-binding protein YhbY -
  PF976_RS13000 (PF976_13000) aroE 2497232..2498074 (-) 843 WP_014470708.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22180.40 Da        Isoelectric Point: 5.4688

>NTDB_id=775045 PF976_RS12965 WP_014470701.1 2493147..2493761(-) (comEA) [Bacillus amyloliquefaciens strain SRCM124317]
MTKVMMTERQRQYLKILGVCLAAGAVLICILLFRSEKEEPVKQQSVLGQTASAPVTDKKDTADDKIMIDIKGAVRHPGVY
EMKAGDRVTQAIKKAGGLKEKADELNVNLAEQLQDGTIVYIPSEGEEANPPKTAAGEKENASVNINTASLDELQAIPGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=775045 PF976_RS12965 WP_014470701.1 2493147..2493761(-) (comEA) [Bacillus amyloliquefaciens strain SRCM124317]
ATGACGAAAGTGATGATGACGGAGCGGCAGAGGCAGTACCTGAAAATTCTTGGCGTTTGTCTTGCGGCAGGGGCGGTTTT
GATCTGCATTTTGCTGTTTCGTTCCGAAAAAGAAGAGCCTGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCGC
CCGTCACGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATATTAAAGGAGCGGTCCGACATCCCGGCGTTTAT
GAAATGAAGGCGGGAGACAGAGTGACGCAAGCCATTAAGAAAGCCGGCGGCCTGAAGGAAAAGGCGGATGAACTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATCGTTTATATCCCGAGTGAAGGCGAAGAGGCGAATCCCCCGAAAACCG
CAGCAGGTGAAAAGGAGAATGCTTCTGTCAATATCAATACGGCATCTCTTGACGAACTTCAGGCGATTCCCGGTGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAATGGGCGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGTTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

57.436

95.588

0.549
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.945

100

0.382
  comEA/celA/cilE Streptococcus pneumoniae Rx1

35.945

100

0.382
  comEA/celA/cilE Streptococcus pneumoniae D39

35.945

100

0.382
  comEA/celA/cilE Streptococcus pneumoniae R6

35.945

100

0.382
  comEA Staphylococcus aureus MW2

35.294

100

0.382
  comEA Latilactobacillus sakei subsp. sakei 23K

33.333

100

0.382
  comEA Staphylococcus aureus N315

34.842

100

0.377
  comEA/celA/cilE Streptococcus mitis NCTC 12261

35.484

100

0.377
  comEA/celA/cilE Streptococcus mitis SK321

35.023

100

0.373