Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   PG909_RS10870 Genome accession   NZ_CP115946
Coordinates   2503824..2505089 (+) Length   421 a.a.
NCBI ID   WP_003273195.1    Uniprot ID   -
Organism   Ralstonia solanacearum MolK2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2498824..2510089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG909_RS10850 ispB 2499284..2500252 (+) 969 WP_003271627.1 octaprenyl diphosphate synthase -
  PG909_RS10860 - 2500527..2501804 (+) 1278 WP_003266026.1 HlyC/CorC family transporter -
  PG909_RS10865 pilB 2502051..2503778 (+) 1728 WP_042550651.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PG909_RS10870 pilG 2503824..2505089 (+) 1266 WP_003273195.1 type II secretion system F family protein Machinery gene
  PG909_RS10875 - 2505092..2505973 (+) 882 WP_003273196.1 prepilin peptidase -
  PG909_RS10880 coaE 2505986..2506600 (+) 615 WP_003273197.1 dephospho-CoA kinase -
  PG909_RS10885 zapD 2506718..2507476 (+) 759 WP_003273198.1 cell division protein ZapD -
  PG909_RS10890 - 2507492..2507707 (+) 216 WP_039569767.1 DNA gyrase inhibitor YacG -
  PG909_RS10895 - 2507700..2508161 (-) 462 WP_003273200.1 NUDIX domain-containing protein -
  PG909_RS10900 - 2508139..2509023 (-) 885 WP_003273202.1 ATP-binding protein -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46482.72 Da        Isoelectric Point: 10.4590

>NTDB_id=774568 PG909_RS10870 WP_003273195.1 2503824..2505089(+) (pilG) [Ralstonia solanacearum MolK2]
MATRAPTANRSAAASARKTAATKAPTQYIFEWEGKDRKGKAFKGEMRAESITEVNAVLRKQGLSITKSKRRRAARGKKIT
PKDVAYFTRQLSTMLKAGVPLLQSVDIIARGHANPNFTQLLTEIRVDIESGSSMAQAFRRHPKYFDSLYCNLIDAGEQGG
ILDALLERLSMYMEKSIALKAQIKSAMIYPISVLTVAFLVTVVLMIFVVPAFKNVFASFGATLPAPTLVVMNISDFFVKW
WYIIIFAPVVGIFLYARAFKRSLKVQRNTHKAILKMPIFGSIIRKATIARWTRTLATMFSAGTPLVESMESVAGAAGNWV
YHDATLEIQQSVRIGTSLTNAMQATHVFDNMVLQMTQIGEESGALDNMLLKVAEFYEREVDDAVANISALIEPIIIVILG
VLIGGMVVAMYLPIFKLGQVV

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=774568 PG909_RS10870 WP_003273195.1 2503824..2505089(+) (pilG) [Ralstonia solanacearum MolK2]
ATGGCCACACGAGCACCGACCGCGAACCGGTCCGCCGCCGCATCGGCACGCAAGACCGCCGCCACGAAGGCGCCCACGCA
GTACATCTTCGAGTGGGAAGGCAAGGACCGCAAAGGCAAAGCCTTCAAGGGCGAGATGCGGGCGGAGAGCATCACCGAAG
TCAACGCGGTGCTGCGCAAGCAGGGGCTGTCGATCACCAAGTCCAAGCGTCGGCGCGCGGCGCGCGGCAAGAAGATCACG
CCCAAGGACGTGGCCTACTTCACGCGCCAGTTGTCGACCATGCTGAAGGCCGGGGTGCCGCTGCTGCAATCGGTCGACAT
CATCGCGCGCGGCCACGCCAACCCGAACTTCACCCAACTGCTGACTGAAATCCGCGTCGACATCGAGTCGGGCAGCAGCA
TGGCGCAGGCTTTCCGCCGCCATCCGAAGTACTTCGATTCGCTGTACTGCAACCTGATCGACGCGGGCGAACAGGGCGGT
ATTCTCGACGCGCTGCTCGAGCGGCTGTCGATGTACATGGAAAAGTCGATCGCGCTGAAGGCGCAGATCAAGTCGGCCAT
GATCTACCCGATCTCGGTGCTGACGGTGGCCTTCCTGGTCACGGTGGTGCTGATGATCTTCGTGGTGCCCGCGTTCAAGA
ACGTGTTCGCCAGCTTCGGCGCCACGCTGCCGGCACCGACGCTGGTGGTGATGAATATCTCGGACTTCTTCGTGAAGTGG
TGGTACATCATCATCTTCGCCCCCGTCGTCGGCATCTTCCTGTATGCGCGGGCCTTCAAGCGCTCGCTGAAGGTGCAGCG
CAACACGCACAAGGCCATCCTGAAGATGCCGATCTTCGGCAGCATCATCCGCAAGGCCACCATCGCGCGCTGGACGCGCA
CGCTGGCGACGATGTTCTCCGCCGGTACGCCGCTGGTGGAATCGATGGAGTCTGTGGCCGGCGCGGCCGGCAACTGGGTC
TACCATGACGCCACCCTTGAGATCCAGCAATCGGTGCGCATCGGTACCAGCCTCACCAACGCCATGCAGGCCACGCACGT
GTTCGACAACATGGTGCTGCAGATGACGCAGATCGGCGAGGAATCCGGTGCGCTGGATAACATGCTGCTGAAGGTAGCCG
AGTTCTACGAGCGCGAGGTCGATGACGCGGTGGCCAACATCTCCGCCCTGATCGAGCCGATCATCATCGTGATCCTCGGC
GTGCTGATCGGCGGCATGGTGGTCGCCATGTACCTGCCGATCTTCAAGCTGGGCCAGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

51.128

94.774

0.485
  pilG Neisseria meningitidis 44/76-A

50.877

94.774

0.482
  pilC Pseudomonas stutzeri DSM 10701

49.752

95.962

0.477
  pilC Acinetobacter baumannii D1279779

47.42

96.675

0.458
  pilC Legionella pneumophila strain ERS1305867

46.914

96.2

0.451
  pilC Acinetobacter baylyi ADP1

46.683

96.675

0.451
  pilC Vibrio campbellii strain DS40M4

38.537

97.387

0.375
  pilC Vibrio cholerae strain A1552

39.25

95.012

0.373