Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PG909_RS10865 Genome accession   NZ_CP115946
Coordinates   2502051..2503778 (+) Length   575 a.a.
NCBI ID   WP_042550651.1    Uniprot ID   -
Organism   Ralstonia solanacearum MolK2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2497051..2508778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG909_RS10835 obgE 2497097..2498197 (-) 1101 WP_003273188.1 GTPase ObgE -
  PG909_RS10840 rpmA 2498396..2498656 (-) 261 WP_003271630.1 50S ribosomal protein L27 -
  PG909_RS10845 rplU 2498694..2499005 (-) 312 WP_003271629.1 50S ribosomal protein L21 -
  PG909_RS10850 ispB 2499284..2500252 (+) 969 WP_003271627.1 octaprenyl diphosphate synthase -
  PG909_RS10860 - 2500527..2501804 (+) 1278 WP_003266026.1 HlyC/CorC family transporter -
  PG909_RS10865 pilB 2502051..2503778 (+) 1728 WP_042550651.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PG909_RS10870 pilG 2503824..2505089 (+) 1266 WP_003273195.1 type II secretion system F family protein Machinery gene
  PG909_RS10875 - 2505092..2505973 (+) 882 WP_003273196.1 prepilin peptidase -
  PG909_RS10880 coaE 2505986..2506600 (+) 615 WP_003273197.1 dephospho-CoA kinase -
  PG909_RS10885 zapD 2506718..2507476 (+) 759 WP_003273198.1 cell division protein ZapD -
  PG909_RS10890 - 2507492..2507707 (+) 216 WP_039569767.1 DNA gyrase inhibitor YacG -
  PG909_RS10895 - 2507700..2508161 (-) 462 WP_003273200.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63806.59 Da        Isoelectric Point: 6.9139

>NTDB_id=774567 PG909_RS10865 WP_042550651.1 2502051..2503778(+) (pilB) [Ralstonia solanacearum MolK2]
MTLGLALTQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPAVLSTNKHF
QQLRLLPLGRRDNRIIVATSDPSDPKPIEELRQKMNVAIETVIVEHDKLMRQLSVANEAPAEIKSLIPKQETTQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKETVDRAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETL
LDAGFKEEDLDGSWQPYHPVGCEACNSSGYKGRVGIYQVMPITEAIQEIILAHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=774567 PG909_RS10865 WP_042550651.1 2502051..2503778(+) (pilB) [Ralstonia solanacearum MolK2]
ATGACTCTCGGACTCGCACTTACGCAGAGCCGGCGAATTGCGCCGACATTACTCACTCAGCTAGAGCAGAGCGCGCGCGA
AAAGCGCACGCAGTTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCGCACGACCTCGCCGTCTTCGTGGCGGGCA
AGTACCAGATGCCGCTGCTGGACCTGGCGCAGTACAAGCTCGACACCGTGCCGGCCGTGCTGTCGACCAACAAGCATTTC
CAGCAACTGCGCTTGCTGCCGCTGGGCCGGCGCGACAACCGCATCATCGTCGCCACCTCGGACCCGAGCGACCCGAAGCC
GATCGAAGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGATGCGCCAGTTGA
GCGTTGCCAACGAAGCGCCGGCCGAGATCAAGTCGCTCATCCCCAAGCAGGAAACCACCCAGATCGAATACGACCCGGGC
GCAGCCGCAGCAGTGCGGCGTACCCCGACCGATGGCATCGACGATGCGCCGGTGGTGCGCTTCCTGCAGAAGCTGTTCAC
CGAGGCGCTGCTGCGCGGCACGTCCGACTTGCACTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTTGAACAAGTCGCGCAACCGCCGCTGGACATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTATCGCGCCTG
GACATCTCGGAAAAACGCGTGCCGCAAGACGGCCGCATGAAGCTGCTGATCACCGTTCCGAAGGACAAGAACGACAAGGA
CAACAAGGAGACGGTCGATCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCGACCGAGCGGCTCGATATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGCAGCTCCTGCTGGAC
GTGATCAAACGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACAAGCTGAACCAGGGCGACATCAACATTTCCACCGCGGAAGACCCGGCGGAAATCCAGTTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGGCTGACCTTCGCCGCGGCGCTGAAATCGTTCCTGCGTCAGGATCCCGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACTGCCGATATCTCCATCAAGGCCGCACAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGACGCGCCGACCACGCTCACACGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCCTCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCTTGCAAGAAACCGGGCCCGCTGCCCAAGGAAACGCTG
CTGGACGCGGGCTTCAAGGAAGAAGACCTGGACGGCTCGTGGCAGCCGTACCATCCGGTCGGCTGCGAGGCATGCAACAG
CAGCGGTTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCGCCCACG
GCACCGCGCTGCAGATCGCCGAACAGGCGCGCAAGGAGGGCGTGCTCTCGCTGCGTGACTCCGGCCTGAGAAAAGTGAAG
CAGGGGCTGACTTCCCTTGAGGAAGTGCTGGCCACGACGAACCAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-133)

Type II/IV secretion system protein (T2SSE)

(184-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

51.413

98.435

0.506
  pilF Neisseria gonorrhoeae MS11

52.747

94.957

0.501
  pilB Acinetobacter baylyi ADP1

49.912

98.261

0.49
  pilB Legionella pneumophila strain ERS1305867

46.127

100

0.466
  pilB Vibrio cholerae strain A1552

44.464

97.391

0.433
  pilB Vibrio campbellii strain DS40M4

44.526

95.304

0.424
  pilB Vibrio parahaemolyticus RIMD 2210633

44.485

94.609

0.421
  pilB/pilB1 Synechocystis sp. PCC 6803

36.258

100

0.381
  pilF Thermus thermophilus HB27

38.104

97.217

0.37