Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   QR290_RS03370 Genome accession   NZ_CP128260
Coordinates   734632..736710 (+) Length   692 a.a.
NCBI ID   WP_115076266.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain PH.SM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 729632..741710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QR290_RS03350 (QR290_03350) comM 731846..732910 (+) 1065 WP_115076263.1 pilus assembly protein PilM Machinery gene
  QR290_RS03355 (QR290_03355) - 732910..733476 (+) 567 WP_115076264.1 PilN domain-containing protein -
  QR290_RS03360 (QR290_03360) pilO 733473..734096 (+) 624 WP_115076265.1 type 4a pilus biogenesis protein PilO Machinery gene
  QR290_RS03365 (QR290_03365) pilP 734093..734617 (+) 525 WP_039766425.1 pilus assembly protein PilP Machinery gene
  QR290_RS03370 (QR290_03370) pilQ 734632..736710 (+) 2079 WP_115076266.1 type IV pilus secretin PilQ Machinery gene
  QR290_RS03375 (QR290_03375) aroK 736715..737233 (+) 519 WP_011332081.1 shikimate kinase AroK -
  QR290_RS03380 (QR290_03380) aroB 737386..738486 (+) 1101 WP_115076267.1 3-dehydroquinate synthase -
  QR290_RS03385 (QR290_03385) - 738495..740096 (+) 1602 WP_289204327.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75040.22 Da        Isoelectric Point: 5.5724

>NTDB_id=773410 QR290_RS03370 WP_115076266.1 734632..736710(+) (pilQ) [Pseudomonas fluorescens strain PH.SM]
MNRIFSTLGFSLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDGPPPQPKGYTTDSPARIALDLPGVASQLASKTRD
LGSGNARTATVVEANNRTRLIISLTQLAPYDTRVEGNTVFVVVGQGSKAAAPRTAARAPRAAPAAAPVRTYTPTARAIRG
VDFQRGTAGEGNVVIDLSDPAIAPDIQEHDGKIILSFARTQLPEPLRVRLDVKDFATPVQFVNAGVSGDRAMITVEPSGT
FDYSTYQTDNKLTVSIRPLTIDDLQKRNADRNSYNGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
VDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSN
GSSTTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=773410 QR290_RS03370 WP_115076266.1 734632..736710(+) (pilQ) [Pseudomonas fluorescens strain PH.SM]
ATGAACAGGATTTTCTCCACCCTCGGTTTCTCGCTATGGATAGCGCTGCTTTCGCCGATGGTACAGGCAGCCAATCTGAA
GGCGCTGGACGTCGCGGCACTGCCGGGCGACCGGGTCGAGCTGAAGCTGTCGTTCGACGGCCCACCCCCGCAGCCAAAGG
GTTACACCACCGATTCGCCGGCGCGGATTGCCCTCGATCTGCCGGGTGTTGCCAGTCAACTGGCGAGCAAGACTCGCGAC
CTGGGCAGTGGCAATGCCCGCACGGCCACGGTGGTCGAGGCCAATAACCGGACGCGGCTGATCATCAGCCTGACGCAACT
GGCGCCTTACGACACCCGGGTCGAAGGCAATACGGTGTTCGTGGTGGTCGGGCAGGGCAGCAAAGCCGCTGCCCCGCGTA
CGGCCGCTCGTGCACCGCGTGCGGCACCTGCGGCAGCGCCGGTCCGCACGTATACGCCGACCGCCAGGGCCATTCGCGGC
GTGGATTTCCAGCGTGGCACGGCGGGCGAAGGCAACGTGGTCATCGATCTGTCCGACCCGGCCATCGCCCCGGACATCCA
GGAACACGACGGCAAGATCATCCTCAGTTTCGCGCGTACCCAATTGCCCGAGCCGCTGCGGGTGCGCCTCGACGTCAAGG
ATTTCGCCACCCCGGTGCAGTTCGTCAATGCCGGTGTCAGCGGTGATCGCGCGATGATCACCGTCGAACCCAGCGGGACT
TTCGACTATTCCACCTACCAGACCGACAACAAACTGACCGTCAGCATCCGGCCGCTGACTATTGATGATCTGCAAAAGCG
CAACGCCGACCGCAACAGCTATAACGGCGAAAAGCTCTCGCTGAATTTTCAGGACATCGATGTGCGTTCGGTGCTGCAAC
TGATCGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGATACGGTACAGGGCGGCATTACCTTGCGCCTGCAGAATGTG
CCGTGGGATCAGGCGCTGGATCTGGTGCTCAAGACCAAAGGCCTGGACAAACGCAAGGTCGGCAACGTGTTGCTGGTGGC
CCCGGCAGATGAAATCGCTGCGCGTGAGCGGCAGGAACTGGAATCGCAGAAACAGATCGCCGAACTGGCGCCGTTGCGCC
GCGAGCTGCTGCAAGTGAACTACGCCAAGGCTGCGGACATTGCCAAGCTGTTCCAGTCGGTCACCAGTGCCGAGGCCAAG
GTCGACGAACGGGGGTCGATCACCGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGACGA
GTTGCGGCGGATCGTGGCGCAGCTGGATATTCCGGTGCGTCAGGTGATGATCGAGGCGCGGATCGTCGAAGCCAACGTCG
ATTACGACAAGAGTCTGGGCGTGCGCTGGGGCGGATCGATCCAGAACAAGGGCAACTGGAACACTTCCGGGGTCAGCAAC
GGCTCTTCGACCACCATCGGCACGCCGGGCAGCACCAGCACCAACTCACCGTTCGTCGACATGGGCACGGTGAACAATAC
GTCCGGGATCGGCATCGCATTCATCACCGACAACGTCTTGCTCGACCTTGAGCTGACCGCGATGGAGAAGACCGGCAACG
GCGAAATCGTCTCGCAGCCCAAGGTGGTCACCTCCGACAAGGAGACTGCGAAGATTCTCAAGGGCACCGAGATTCCGTAT
CAGGAAGCCAGCTCCAGCGGCGCTACATCGGTGTCTTTCAAGGAGGCCTCGCTGTCGCTGGAAGTGACGCCGCAGATCAC
CCCCGATAACCGCATCATCATGGAGGTGAAGGTCACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGGATGTACCGC
CGATCAAGAAGAACGAGGTCAATGCCAAGGTGCTGGTGAACGACGGCGAGACCATCGTGATCGGGGGCGTTTTCTCAAAT
ACTCAAAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterProScan.

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

AMIN domain (AMIN)

(37-121)

Bacterial type II and III secretion system protein (Secretin)

(527-683)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.827

100

0.744
  pilQ Acinetobacter baumannii D1279779

37.784

100

0.409
  pilQ Acinetobacter baumannii strain A118

37.784

100

0.409
  comQ/pilQ Acinetobacter baylyi ADP1

40.778

100

0.409
  pilQ Legionella pneumophila strain Lp02

37.481

97.543

0.366
  pilQ Legionella pneumophila strain ERS1305867

37.444

97.254

0.364