Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   QR290_RS03350 Genome accession   NZ_CP128260
Coordinates   731846..732910 (+) Length   354 a.a.
NCBI ID   WP_115076263.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain PH.SM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 726846..737910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QR290_RS03340 (QR290_03340) - 727561..728829 (+) 1269 WP_115076261.1 malic enzyme-like NAD(P)-binding protein -
  QR290_RS03345 (QR290_03345) - 729181..731628 (-) 2448 WP_115076262.1 penicillin-binding protein 1A -
  QR290_RS03350 (QR290_03350) comM 731846..732910 (+) 1065 WP_115076263.1 pilus assembly protein PilM Machinery gene
  QR290_RS03355 (QR290_03355) - 732910..733476 (+) 567 WP_115076264.1 PilN domain-containing protein -
  QR290_RS03360 (QR290_03360) pilO 733473..734096 (+) 624 WP_115076265.1 type 4a pilus biogenesis protein PilO Machinery gene
  QR290_RS03365 (QR290_03365) pilP 734093..734617 (+) 525 WP_039766425.1 pilus assembly protein PilP Machinery gene
  QR290_RS03370 (QR290_03370) pilQ 734632..736710 (+) 2079 WP_115076266.1 type IV pilus secretin PilQ Machinery gene
  QR290_RS03375 (QR290_03375) aroK 736715..737233 (+) 519 WP_011332081.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38168.68 Da        Isoelectric Point: 4.7539

>NTDB_id=773407 QR290_RS03350 WP_115076263.1 731846..732910(+) (comM) [Pseudomonas fluorescens strain PH.SM]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPASAVVEKNIAELEGVGQALSRVLVKARTGLKTVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGFSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQDRLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEERLHTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=773407 QR290_RS03350 WP_115076263.1 731846..732910(+) (comM) [Pseudomonas fluorescens strain PH.SM]
GTGCTAGGACTCTTCAATAAAAAGGCCAATACGTTACTGGGGATCGACATCAGCTCCACTTCGGTGAAGCTGCTGGAGCT
GAGCCGTCAGGGTGAGCGCTACCGGGTCGAGGCATACGCGGTGGAGCCGCTGCCGGCGAGCGCCGTGGTCGAAAAGAACA
TCGCCGAGCTCGAGGGCGTCGGCCAGGCCCTGTCCCGGGTGCTGGTCAAGGCGCGCACCGGGCTGAAAACCGTGGCGGTG
GCCGTGGCCGGTTCGGCGGTGATCACCAAGGTCATCGAGATGGACGCCGGGCTGTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATTCCCTATCCGCTGGACGAGGTCGCCATCGACTTCGAAGTCCAGGGCTTTTCGCCGC
GCAACCCCGAGCGGGTCAACGTGCTGCTGGCCGCCTGCCGCAAGGAAAACGTCGAGGTCCGCGAAGCGGCGCTAGCACTG
GCCGGGCTGACGGCGCGGGTGGTGGATGTCGAAGCCTATGCGCTGGAGCGCTCGTTCGGCCTGCTGGCCACGCAACTGGC
GGCCTCCCAGGATCGACTGACGGTGGCCGTGGTCGACATCGGCGCCACCATGACCACCCTCAGCGTCCTGCACAACGGCC
GGATCATCTATACCCGCGAACAATTGTTCGGCGGCCGACAGCTGACCGAGGAAATCCAGCGCCGTTATGGCCTGACCCTT
GAACAGGCGGGGCTCGCCAAGAAGCAGGGCGGCCTGCCGGACGATTACGTCAGCGAGGTGCTGCAGCCGTTTCGCGAGGC
ACTGGTGCAGCAGGTGTCGCGCTCCCTGCAGTTCTTTTTCGCCTCCGGCCAGTACAACGCTGTCGACCATATTCTGCTGG
CCGGCGGCACGGCGTCGGTGCCCGGGCTCGACCGGCTGATCGAAGAACGCCTGCACACCCCGACCCAGGTCGCCAACCCG
TTTTCCGACATGGCCTTGAGCAGCAAGGTCAACGCCGGGGCTTTGGCGAGCGACGCGCCGGCCCTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM/pilM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

49.577

100

0.497