Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   QR290_RS03360 Genome accession   NZ_CP128260
Coordinates   733473..734096 (+) Length   207 a.a.
NCBI ID   WP_115076265.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain PH.SM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 728473..739096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QR290_RS03345 (QR290_03345) - 729181..731628 (-) 2448 WP_115076262.1 penicillin-binding protein 1A -
  QR290_RS03350 (QR290_03350) comM 731846..732910 (+) 1065 WP_115076263.1 pilus assembly protein PilM Machinery gene
  QR290_RS03355 (QR290_03355) - 732910..733476 (+) 567 WP_115076264.1 PilN domain-containing protein -
  QR290_RS03360 (QR290_03360) pilO 733473..734096 (+) 624 WP_115076265.1 type 4a pilus biogenesis protein PilO Machinery gene
  QR290_RS03365 (QR290_03365) pilP 734093..734617 (+) 525 WP_039766425.1 pilus assembly protein PilP Machinery gene
  QR290_RS03370 (QR290_03370) pilQ 734632..736710 (+) 2079 WP_115076266.1 type IV pilus secretin PilQ Machinery gene
  QR290_RS03375 (QR290_03375) aroK 736715..737233 (+) 519 WP_011332081.1 shikimate kinase AroK -
  QR290_RS03380 (QR290_03380) aroB 737386..738486 (+) 1101 WP_115076267.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 23131.54 Da        Isoelectric Point: 4.5240

>NTDB_id=773408 QR290_RS03360 WP_115076265.1 733473..734096(+) (pilO) [Pseudomonas fluorescens strain PH.SM]
MKPSEWLQSLRNVDFNDLDTSNIGSWPPVVKSLAGGLLMVLVLALGYNFFISDLESQLELKREEETTLKEQFASKAHMAA
NLELYTQQMKEMENSFGVLLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQITVTGAYHDLATF
VSGVAGLPRIVTLHDFELAPANPEGGPKLRMSILAKTYRYNDKGLQK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=773408 QR290_RS03360 WP_115076265.1 733473..734096(+) (pilO) [Pseudomonas fluorescens strain PH.SM]
ATGAAGCCGTCCGAGTGGTTGCAAAGCCTGCGCAATGTCGACTTCAACGATCTGGACACCAGCAACATCGGCTCCTGGCC
GCCAGTGGTCAAAAGCCTGGCCGGCGGGCTGTTGATGGTGTTGGTTTTGGCGCTTGGCTATAACTTTTTCATCAGTGATC
TGGAGAGTCAGCTCGAGCTCAAGCGTGAGGAGGAGACCACCCTCAAGGAGCAGTTCGCGAGCAAGGCGCACATGGCGGCC
AATCTGGAGCTGTACACCCAGCAGATGAAAGAGATGGAAAACTCGTTCGGCGTGCTGTTGCGGCAACTGCCCAGTGACAC
CGAAGTGCCGGGGTTGCTGGAAGACATCACCCGCACCGGGCTGGGCAGCGGTCTTGAATTCGAAGAGATCAAGCTGCTGC
CGGAGGTCACCCAGCAGTTCTACATCGAGTTGCCCATCCAGATCACCGTGACCGGTGCCTATCACGACCTCGCGACCTTC
GTCAGCGGCGTGGCCGGGCTCCCACGGATCGTCACCCTGCACGATTTCGAGCTCGCGCCGGCCAATCCCGAAGGCGGGCC
GAAGTTGCGCATGAGCATTCTCGCCAAGACCTACCGCTACAACGACAAGGGGCTGCAAAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(59-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

45.263

91.787

0.415
  comO/pilO Acinetobacter baylyi ADP1

44.974

91.304

0.411
  pilO Legionella pneumophila strain ERS1305867

43.523

93.237

0.406