Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O8413_RS08965 Genome accession   NZ_CP115188
Coordinates   1947336..1949024 (+) Length   562 a.a.
NCBI ID   WP_283512448.1    Uniprot ID   -
Organism   Vibrio furnissii strain MT14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1942336..1954024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O8413_RS08935 (O8413_08935) dsbC 1942656..1943420 (-) 765 WP_158107763.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  O8413_RS08940 (O8413_08940) xerD 1943437..1944345 (-) 909 WP_283512447.1 site-specific tyrosine recombinase XerD -
  O8413_RS08945 (O8413_08945) fldB 1944486..1945007 (+) 522 WP_004729075.1 flavodoxin FldB -
  O8413_RS08950 (O8413_08950) ampD 1945043..1945597 (-) 555 WP_158140199.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  O8413_RS08955 (O8413_08955) nadC 1945746..1946633 (+) 888 WP_154180385.1 carboxylating nicotinate-nucleotide diphosphorylase -
  O8413_RS08960 (O8413_08960) - 1946880..1947329 (+) 450 WP_055466517.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  O8413_RS08965 (O8413_08965) pilB 1947336..1949024 (+) 1689 WP_283512448.1 type IV-A pilus assembly ATPase PilB Machinery gene
  O8413_RS08970 (O8413_08970) pilC 1949103..1950329 (+) 1227 WP_283512449.1 type II secretion system F family protein Machinery gene
  O8413_RS08975 (O8413_08975) pilD 1950381..1951250 (+) 870 WP_004729087.1 A24 family peptidase Machinery gene
  O8413_RS08980 (O8413_08980) coaE 1951253..1951861 (+) 609 WP_283512450.1 dephospho-CoA kinase -
  O8413_RS08985 (O8413_08985) zapD 1951894..1952634 (+) 741 WP_283512451.1 cell division protein ZapD -
  O8413_RS08990 (O8413_08990) yacG 1952692..1952889 (+) 198 WP_004729092.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62106.25 Da        Isoelectric Point: 6.1994

>NTDB_id=768803 O8413_RS08965 WP_283512448.1 1947336..1949024(+) (pilB) [Vibrio furnissii strain MT14]
MLTNLIAILRQAKLISPTQEQAVMDHIHASGATVPEALIDLAIFHHHDLTEQLSQIFGLPETDLTRFDYAPLCQQLGLRE
LITRYQALPLHSNGQILTLAVADPTHLQVEDEFRFATGLQVELVLADHKALHAAIRRLYGRSLATPGTQGKEISQDELAD
LVQISDDEKTTIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESHYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLDADTAIDMRVSTLPTLWGEKIVLRLLDSSAANLNIDKLGYNEQQKQLYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTEQVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAIKAAQTGHLVLSTLHTNSAAETVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKVAITPPAALQQQFGLLAED
TVYEAHPSGCTECTNGYSGRVGIYEVMAFHPALADAVMQRASIHQIETIAQQHGMQPLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=768803 O8413_RS08965 WP_283512448.1 1947336..1949024(+) (pilB) [Vibrio furnissii strain MT14]
ATGCTGACCAACCTTATCGCCATTCTGCGTCAGGCTAAGTTGATCAGCCCAACGCAGGAACAGGCCGTGATGGATCATAT
TCACGCTTCCGGTGCCACGGTGCCGGAGGCGTTGATCGATTTGGCGATATTTCATCATCACGATTTAACCGAGCAACTAA
GTCAGATCTTTGGCCTACCCGAAACCGATCTCACTCGGTTTGATTACGCGCCATTGTGCCAGCAGTTGGGACTTCGAGAA
CTCATCACCCGCTACCAAGCCCTGCCGCTGCACAGCAATGGCCAAATCCTGACGTTGGCGGTTGCTGACCCAACTCACCT
GCAAGTCGAAGACGAGTTTCGCTTTGCCACCGGATTACAGGTGGAACTGGTGCTCGCCGATCACAAAGCGCTGCACGCCG
CCATTCGCCGCTTGTATGGGCGCAGCCTCGCGACGCCGGGGACGCAAGGCAAAGAGATCAGTCAGGATGAATTGGCGGAT
CTGGTACAGATCTCTGACGACGAAAAAACCACCATTGAAGATCTCAGCCAAGATGATTCACCGGTCAGTCGTTTTATCAA
CCAGATCCTACTCGATGCCGTGCGCAAAGGTGCGTCCGACATTCACTTTGAGCCGTATGAGTCCCACTACCGTATTCGTC
TGCGCTGTGACGGCATTCTGGTCGAAACCCAACAACCTGCCAGTCACCTCAGCCGACGCTTGGCAGCCCGAATCAAAATT
TTGTCGAAATTAGATATTGCCGAGCGCCGCCTCCCGCAAGATGGACGCATCAAACTGCGCTTGGATGCCGACACCGCGAT
TGATATGCGTGTATCGACCTTGCCAACATTGTGGGGTGAAAAGATTGTTCTGCGCTTACTCGATAGCAGCGCGGCCAACC
TCAACATCGACAAACTCGGCTACAACGAACAGCAGAAACAGCTCTACCTCGCGGCCCTCACCAAACCGCAAGGCATGATC
TTAATGACCGGCCCAACCGGCAGCGGCAAAACTGTGTCGCTCTACACCGGATTACGCATTCTCAATACCGAACAAGTGAA
CATCTCCACCGCCGAAGATCCGGTGGAAATCAACCTGCCCGGCATCAATCAGGTGCAAGTTCAACCCAAAATTGGCTTTG
GCTTTGCACAAGCGCTGCGCTCGTTTCTACGTCAAGATCCCGATGTGGTGATGGTGGGAGAAATCCGCGATCTAGAAACC
GCAGAAATCGCCATTAAGGCCGCGCAAACCGGGCACTTGGTGCTCTCAACCTTGCACACCAACTCGGCCGCGGAAACCGT
GATTCGTTTAGCCAACATGGGCATTGAACCCTTTAACCTTGCCTCATCATTGAGCCTGATCATTGCGCAGCGGCTGGCCC
GCCGTCTGTGCCCGCATTGCAAGGTCGCCATCACGCCCCCAGCTGCGCTGCAGCAGCAATTTGGCTTGCTCGCCGAAGAT
ACTGTATACGAAGCACATCCATCGGGTTGCACGGAATGCACCAATGGGTATAGCGGCCGCGTGGGCATTTATGAAGTGAT
GGCGTTTCATCCCGCCTTGGCGGACGCGGTGATGCAGCGCGCCAGCATTCATCAAATCGAAACCATTGCCCAACAGCACG
GCATGCAACCCCTGCTGCAATCGGGGATTGAAAAACTGCGCGCGGGCATCACCAGTTTTAGTGAATTGCAGCGAGTACTC
TACTTTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-142)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

82.384

100

0.824
  pilB Vibrio campbellii strain DS40M4

71.886

100

0.719
  pilB Vibrio parahaemolyticus RIMD 2210633

71.708

100

0.717
  pilB Legionella pneumophila strain ERS1305867

52.519

91.815

0.482
  pilB Acinetobacter baumannii D1279779

51.06

92.349

0.472
  pilB Acinetobacter baylyi ADP1

50.097

91.993

0.461
  pilF Neisseria gonorrhoeae MS11

44.858

100

0.45
  pilB Haemophilus influenzae 86-028NP

39.658

93.772

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

37.636

97.865

0.368
  pilF Thermus thermophilus HB27

36.655

100

0.367