Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O6P33_RS10180 Genome accession   NZ_CP114976
Coordinates   2171318..2173012 (-) Length   564 a.a.
NCBI ID   WP_269817673.1    Uniprot ID   A0AAF0AI78
Organism   Denitrificimonas caeni strain CE14     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2166318..2178012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O6P33_RS10155 (O6P33_10155) rluC 2166644..2167612 (-) 969 WP_269817668.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
  O6P33_RS10160 (O6P33_10160) nth 2167822..2168460 (+) 639 WP_269817669.1 endonuclease III -
  O6P33_RS10165 (O6P33_10165) - 2168566..2168754 (+) 189 WP_420094994.1 PA3496 family putative envelope integrity protein -
  O6P33_RS10170 (O6P33_10170) pilD 2168999..2169868 (-) 870 WP_269817671.1 prepilin peptidase Machinery gene
  O6P33_RS10175 (O6P33_10175) pilC 2170097..2171314 (-) 1218 WP_269817672.1 type II secretion system F family protein Machinery gene
  O6P33_RS10180 (O6P33_10180) pilB 2171318..2173012 (-) 1695 WP_269817673.1 type IV-A pilus assembly ATPase PilB Machinery gene
  O6P33_RS10185 (O6P33_10185) comP 2173497..2173904 (+) 408 WP_269819514.1 pilin Machinery gene
  O6P33_RS10190 (O6P33_10190) - 2174104..2174424 (+) 321 WP_269817674.1 excalibur calcium-binding domain-containing protein -
  O6P33_RS10195 (O6P33_10195) - 2174894..2175895 (+) 1002 WP_269817675.1 hypothetical protein -
  O6P33_RS10200 (O6P33_10200) - 2176101..2176469 (-) 369 WP_269817676.1 translation initiation factor Sui1 -
  O6P33_RS10205 (O6P33_10205) - 2176549..2177004 (-) 456 WP_269817677.1 methyltransferase family protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 61902.90 Da        Isoelectric Point: 5.3230

>NTDB_id=767654 O6P33_RS10180 WP_269817673.1 2171318..2173012(-) (pilB) [Denitrificimonas caeni strain CE14]
MNDSVNLVGLARQVVEAGLLDEKKAQDAVQQAKRNQTPLVTWLVQNKLVKSRVLMELASDQFGVAYFDLSAVNAEVFPKD
LVSEKLALQHRVLPLYKRANKLYVAISDPGNHQAITDVQFSTGMTVEPLLVEDDKLGQAIDKLYESATGGLEGMDDVELD
GLEVADEDGKAAATTDDADETPVVRFVHKMLLDAIKGGSSDLHFEPYEKMYRVRFRTDGVLHEVAKPPIQLRDRISARLK
VMASMDISERRKPQDGRIKLKVSANKSIDFRVNTLPTLWGEKIVMRILDSSSAKMGIDALGYEEDQKQLYLDALKQPQGM
ILVTGPTGSGKTVSLYTGLNILNTIDVNISTAEDPVEINLEGINQVNVNPKQGMDFTTALRAFLRQDPDVIMVGEIRDLE
TAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNLATSVNLIIAQRLARKLCACKQEVTVPNDVLLNEGFSPEQ
VGTFKVYGPKGCDNCNGGYKGRVGIYEVVKSTPALQRIIMEDGNSIDIDAQMRKDGFNSLRASGLLKVIQGVTSLAEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=767654 O6P33_RS10180 WP_269817673.1 2171318..2173012(-) (pilB) [Denitrificimonas caeni strain CE14]
ATGAATGACAGTGTGAATTTAGTTGGCCTCGCCCGGCAGGTGGTTGAGGCTGGTTTGTTAGATGAGAAGAAAGCACAAGA
CGCGGTACAGCAGGCAAAGCGCAATCAAACACCCTTAGTCACTTGGTTGGTACAAAATAAACTGGTTAAAAGTCGCGTGT
TGATGGAGTTGGCTTCTGATCAGTTTGGTGTGGCGTATTTTGATCTATCTGCGGTCAATGCCGAAGTCTTCCCGAAAGAT
TTAGTCAGTGAAAAACTCGCGCTGCAGCATCGAGTGCTGCCGCTTTATAAGCGCGCTAATAAACTTTATGTGGCTATATC
TGACCCAGGTAATCACCAAGCCATTACGGATGTGCAGTTCAGTACCGGGATGACGGTTGAGCCGCTGCTAGTGGAAGACG
ACAAGCTAGGGCAGGCCATTGATAAGCTCTATGAGTCGGCTACTGGCGGCTTAGAGGGTATGGATGATGTTGAGCTGGAT
GGCTTAGAAGTAGCGGATGAGGATGGTAAGGCCGCCGCTACCACTGATGATGCTGATGAAACACCTGTGGTGCGTTTTGT
GCATAAAATGCTGCTGGACGCAATTAAAGGCGGCTCGTCGGATTTGCACTTTGAGCCTTATGAGAAAATGTATCGTGTGC
GTTTTCGTACCGATGGTGTCTTGCATGAAGTAGCTAAACCGCCTATCCAGTTACGCGACCGTATTTCTGCGCGTTTAAAA
GTTATGGCCAGTATGGATATTTCTGAGCGCCGTAAGCCGCAAGATGGTCGGATCAAGTTAAAAGTCTCTGCCAATAAGTC
CATCGACTTTCGAGTGAATACCTTGCCAACTTTGTGGGGTGAGAAGATCGTGATGCGTATCCTCGACTCCAGCAGTGCGA
AAATGGGTATTGATGCGTTGGGATATGAAGAAGATCAAAAGCAGTTGTATCTGGATGCGCTAAAACAGCCGCAAGGAATG
ATTCTGGTCACGGGGCCGACCGGCTCTGGTAAAACTGTTTCGTTGTATACCGGTTTGAATATCCTAAATACCATTGATGT
GAATATTTCTACCGCGGAAGATCCGGTGGAAATTAACTTGGAAGGGATTAACCAGGTTAACGTGAACCCTAAGCAGGGCA
TGGACTTTACTACGGCGCTGCGGGCCTTCTTGCGCCAAGACCCGGACGTGATCATGGTCGGGGAAATTCGAGATTTAGAA
ACCGCAGAAATTGCCATTAAAGCAGCACAAACCGGGCACATGGTGATGTCCACCTTGCACACCAACAGTGCCGCAGAAAC
TTTGACCCGCTTGCGTAATATGGGGGTGGCGGCGTTTAACCTAGCAACCTCGGTGAACTTGATTATTGCCCAGCGCTTGG
CGCGTAAACTCTGCGCTTGTAAACAAGAAGTGACAGTGCCGAATGATGTGCTATTAAACGAAGGCTTTAGCCCAGAACAG
GTGGGCACTTTTAAAGTGTACGGGCCAAAAGGTTGTGATAATTGCAATGGCGGCTACAAAGGTCGTGTCGGAATCTATGA
AGTAGTTAAAAGCACGCCTGCCCTACAAAGGATTATCATGGAGGACGGCAACTCCATCGATATTGATGCACAGATGCGTA
AAGATGGCTTTAACAGCTTACGCGCTTCGGGATTGCTGAAAGTTATTCAAGGCGTGACCAGTCTCGCAGAAGTTAACCGT
GTTACTAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.019

100

0.598
  pilB Acinetobacter baylyi ADP1

57.245

99.113

0.567
  pilB Legionella pneumophila strain ERS1305867

52.381

100

0.527
  pilB Vibrio parahaemolyticus RIMD 2210633

50.622

99.823

0.505
  pilB Vibrio cholerae strain A1552

50.712

99.645

0.505
  pilB Vibrio campbellii strain DS40M4

50

99.291

0.496
  pilF Neisseria gonorrhoeae MS11

47.865

99.645

0.477
  pilB/pilB1 Synechocystis sp. PCC 6803

36.634

100

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.738

98.936

0.363