NTDB

Overview

Name	pilD	Type	Machinery gene
Locus tag	O6P33_RS10170	Genome accession	NZ_CP114976
Coordinates	2168999..2169868 (-)	Length	289 a.a.
NCBI ID	WP_269817671.1	Uniprot ID	A0AAE9VMY0
Organism	Denitrificimonas caeni strain CE14
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2163999..2174868

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
O6P33_RS10145 (O6P33_10145)	-	2165275..2165904 (-)	630	WP_269817665.1	alpha/beta hydrolase	-
O6P33_RS10150 (O6P33_10150)	-	2166057..2166503 (+)	447	WP_269817667.1	YhcB family protein	-
O6P33_RS10155 (O6P33_10155)	rluC	2166644..2167612 (-)	969	WP_269817668.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
O6P33_RS10160 (O6P33_10160)	nth	2167822..2168460 (+)	639	WP_269817669.1	endonuclease III	-
O6P33_RS10165 (O6P33_10165)	-	2168566..2168754 (+)	189	WP_420094994.1	PA3496 family putative envelope integrity protein	-
O6P33_RS10170 (O6P33_10170)	pilD	2168999..2169868 (-)	870	WP_269817671.1	prepilin peptidase	Machinery gene
O6P33_RS10175 (O6P33_10175)	pilC	2170097..2171314 (-)	1218	WP_269817672.1	type II secretion system F family protein	Machinery gene
O6P33_RS10180 (O6P33_10180)	pilB	2171318..2173012 (-)	1695	WP_269817673.1	type IV-A pilus assembly ATPase PilB	Machinery gene
O6P33_RS10185 (O6P33_10185)	comP	2173497..2173904 (+)	408	WP_269819514.1	pilin	Machinery gene
O6P33_RS10190 (O6P33_10190)	-	2174104..2174424 (+)	321	WP_269817674.1	excalibur calcium-binding domain-containing protein	-

Sequence

Protein

Download Length: 289 a.a. Molecular weight: 31816.87 Da Isoelectric Point: 5.2344

                                >NTDB_id=767652 O6P33_RS10170 WP_269817671.1 2168999..2169868(-) (pilD) [Denitrificimonas caeni strain CE14]

MSILEFWGANAAVFVACVVVLGLLVGSFLNVVVYRLPKMLMRDWRAQAREVLEMPTKDVEETFNLVLPNSSCPNCGHEIK

PWENIPLLSWLFLRGKCSGCKQAISVRYPLVELACGVLSGMIAWQFGVSWETLAMLVLTWGLLAMSLIDADQQILPDALV

LPLLWLGLILNSFGLFASLPDALWGAVIGYMSLWSIFWLFKLVTGKEGMGYGDFKLLALLGAWGGWQVLPLTILLSSVVG

AVLGVIILRLQRNSYSNPIPFGPYLAVAGWIALIWGDAITSSYLQFAGF

Nucleotide

Download Length: 870 bp

                                >NTDB_id=767652 O6P33_RS10170 WP_269817671.1 2168999..2169868(-) (pilD) [Denitrificimonas caeni strain CE14]

ATGAGTATTCTTGAGTTTTGGGGCGCAAATGCGGCGGTGTTTGTTGCCTGTGTGGTGGTGCTAGGTTTGTTGGTTGGTAG

TTTTTTGAATGTGGTGGTGTATCGCTTACCCAAGATGCTCATGCGGGACTGGCGTGCTCAGGCGCGTGAAGTGCTGGAAA

TGCCCACGAAAGATGTTGAAGAGACATTTAATTTGGTGCTGCCCAACTCCAGTTGCCCCAATTGTGGTCATGAGATAAAA

CCTTGGGAAAATATTCCGCTGCTGAGTTGGTTGTTTTTGCGCGGTAAGTGCTCAGGTTGTAAGCAAGCCATTAGCGTGCG

TTATCCGCTGGTGGAGCTTGCCTGTGGTGTGTTGTCCGGTATGATTGCTTGGCAGTTTGGCGTGAGTTGGGAAACTTTGG

CGATGCTGGTGCTGACTTGGGGCTTGCTGGCCATGAGTTTGATTGACGCTGATCAGCAGATATTGCCCGACGCTTTGGTG

TTGCCGCTGCTGTGGTTGGGGTTGATTTTAAATAGCTTTGGTTTGTTTGCCAGTTTGCCGGATGCTCTGTGGGGCGCGGT

GATTGGCTATATGAGTTTGTGGTCGATTTTTTGGCTGTTTAAGCTGGTGACCGGTAAAGAAGGCATGGGTTATGGCGACT

TTAAGTTGCTGGCGTTGCTGGGTGCCTGGGGTGGCTGGCAAGTGTTGCCTTTGACAATCTTATTGTCGTCTGTGGTAGGG

GCGGTGCTGGGGGTGATTATTTTGCGTTTGCAGCGCAACAGCTACAGCAACCCCATTCCTTTTGGCCCTTATTTAGCTGT

AGCTGGTTGGATTGCGCTGATTTGGGGCGATGCAATTACCAGCAGTTATTTGCAGTTTGCCGGATTTTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Type IV leader peptidase family (Peptidase_A24)

(136-245)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilD	Vibrio cholerae strain A1552	53.65	94.81	0.509
pilD	Vibrio campbellii strain DS40M4	52.92	94.81	0.502
pilD	Neisseria gonorrhoeae MS11	51.88	92.042	0.478
pilD	Acinetobacter nosocomialis M2	47.719	98.616	0.471
pilD	Acinetobacter baumannii D1279779	47.719	98.616	0.471

Detailed information NTG0767652