Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O4D10_RS05720 Genome accession   NZ_CP114795
Coordinates   1343562..1345298 (+) Length   578 a.a.
NCBI ID   WP_386341199.1    Uniprot ID   -
Organism   Xanthomonas citri pv. citri strain CM-BD-sm     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1338562..1350298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O4D10_RS05695 (O4D10_05685) coaE 1339483..1340094 (-) 612 WP_386341197.1 dephospho-CoA kinase -
  O4D10_RS05700 (O4D10_05690) - 1340108..1340971 (-) 864 WP_003491180.1 A24 family peptidase -
  O4D10_RS05705 (O4D10_05695) pilC 1340978..1342237 (-) 1260 WP_033836749.1 type II secretion system F family protein Machinery gene
  O4D10_RS05710 (O4D10_05700) comP 1342582..1343010 (+) 429 WP_005921364.1 pilin Machinery gene
  O4D10_RS05715 (O4D10_05705) pilA2 1343107..1343520 (+) 414 WP_005921365.1 pilin Machinery gene
  O4D10_RS05720 (O4D10_05710) pilB 1343562..1345298 (+) 1737 WP_386341199.1 type IV-A pilus assembly ATPase PilB Machinery gene
  O4D10_RS05725 (O4D10_05715) pilB 1345395..1345514 (+) 120 WP_005915819.1 hypothetical protein Machinery gene
  O4D10_RS05730 - 1345596..1345778 (+) 183 Protein_1131 hypothetical protein -
  O4D10_RS05735 (O4D10_05720) pilR 1345964..1347418 (-) 1455 WP_033484414.1 sigma-54 dependent transcriptional regulator Regulator
  O4D10_RS05740 (O4D10_05725) - 1347683..1349296 (-) 1614 WP_386341202.1 sensor histidine kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62684.78 Da        Isoelectric Point: 5.7586

>NTDB_id=767151 O4D10_RS05720 WP_386341199.1 1343562..1345298(+) (pilB) [Xanthomonas citri pv. citri strain CM-BD-sm]
MNVAIGSNLVGITGIARRLVQDGALDEASARAAMDHATQAKVPLPQWFAEKKLVTAAQLAAANAVEFGMPLMDVSAFDAN
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=767151 O4D10_RS05720 WP_386341199.1 1343562..1345298(+) (pilB) [Xanthomonas citri pv. citri strain CM-BD-sm]
ATGAACGTTGCAATCGGCTCAAATCTAGTTGGAATTACTGGTATTGCGCGGCGCCTTGTTCAGGATGGTGCGCTTGATGA
GGCGAGCGCGCGTGCGGCAATGGATCACGCAACCCAGGCCAAAGTTCCTTTACCGCAATGGTTCGCCGAAAAGAAGCTTG
TAACAGCCGCACAACTCGCGGCCGCCAACGCAGTCGAGTTTGGCATGCCTTTGATGGATGTATCGGCGTTCGATGCCAAC
CAGAACGCGGTCAAGCTGGTCAGTGAAGAGTTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAATCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACGAACTTGGTGGTCGAGGCCA
TTCTTGTCGACGAGGATCAGATCCGCCGGACGCTCGAACAGTGGCAGGCCAGCAACGCGTCGTTGGGTTCGTCGCTTGGC
AACGACGACGAGGAGATGGGGGATCTGGACGTCTCCACCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGCCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGGTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAGCAGAAG
CTGTTCCTGGAAGCGATCCACAAGCCCTATGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTACACCGCGCTGGGAATTCTCAACGACGAGACGCGCAATATCTCCACCGCGGAGGATCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTCGCCGCGGCTTTGCGCTCGTTCCTGCGCCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGTCACATGGT
GCTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGTCTGATGAACATGGGAATCGCGCCATACAACATCA
CCTCGTCGGTGACGCTGGTGATCGCGCAGCGTCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCATTGCTGGCCGAAGGGTTCACGCCTGCACAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
TGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCAATCGTGC
TGGAAGGCGGCAACGCGATGCAGATCGCCGAGGCGGCGCAGGCGATCGGTATCCGTGATTTGCGGCAGTCGGCGTTGATG
AAGGCTGCGCACGGGGTGACCAGCCTGGCCGAGATCAATCGTGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.162

98.27

0.552
  pilB Acinetobacter baylyi ADP1

55.282

98.27

0.543
  pilB Legionella pneumophila strain ERS1305867

51.557

100

0.516
  pilB Vibrio cholerae strain A1552

48.962

100

0.49
  pilF Neisseria gonorrhoeae MS11

49.296

98.27

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

45.745

97.578

0.446
  pilB Vibrio campbellii strain DS40M4

44.991

98.443

0.443