Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O4M77_RS13210 Genome accession   NZ_CP114606
Coordinates   2776016..2777725 (+) Length   569 a.a.
NCBI ID   WP_159122714.1    Uniprot ID   -
Organism   Acinetobacter sp. YWS30-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2771016..2782725
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O4M77_RS13165 (O4M77_13155) nusA 2771595..2773079 (-) 1485 WP_004787201.1 transcription termination factor NusA -
  O4M77_RS13170 (O4M77_13160) rimP 2773115..2773639 (-) 525 WP_179992192.1 ribosome maturation factor RimP -
  O4M77_RS13200 (O4M77_13190) secG 2774603..2774932 (-) 330 WP_323713551.1 preprotein translocase subunit SecG -
  O4M77_RS13205 (O4M77_13195) tpiA 2774946..2775737 (-) 792 WP_159122713.1 triose-phosphate isomerase -
  O4M77_RS13210 (O4M77_13200) pilB 2776016..2777725 (+) 1710 WP_159122714.1 type IV-A pilus assembly ATPase PilB Machinery gene
  O4M77_RS13215 (O4M77_13205) pilC 2777756..2778985 (+) 1230 WP_323713552.1 type II secretion system F family protein Machinery gene
  O4M77_RS13220 (O4M77_13210) pilD 2778985..2779845 (+) 861 WP_180002777.1 prepilin peptidase Machinery gene
  O4M77_RS13225 (O4M77_13215) coaE 2779847..2780452 (+) 606 WP_159122717.1 dephospho-CoA kinase -
  O4M77_RS13230 (O4M77_13220) - 2780491..2781408 (-) 918 WP_323713553.1 DMT family transporter -
  O4M77_RS13235 (O4M77_13225) rlmB 2781452..2782201 (-) 750 WP_005233526.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  O4M77_RS13240 (O4M77_13230) - 2782344..2782667 (-) 324 WP_004787221.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63419.51 Da        Isoelectric Point: 4.8353

>NTDB_id=766850 O4M77_RS13210 WP_159122714.1 2776016..2777725(+) (pilB) [Acinetobacter sp. YWS30-1]
MTALQGSPRFTGFIRRLVDEGVLSAEDMRTALANAKQEKTDIVAYLVSHHNLSPTTIAETISVEFGEPLFDIGAYDPGQI
LREDIDEKLITKHRILPIYKNANILYVATSNPTNIEATDAIRFATKLNIETVIVEHNKLEKLIEQNFTEESTFDFDDDFD
LDVNVDTADPTQEEDEKDKGEEAPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIATPPLQLANRLASR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKELFMEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFAAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPKQSLLEMGFT
EADLQSPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIEIAEASARAGFNNLRRSGLVKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=766850 O4M77_RS13210 WP_159122714.1 2776016..2777725(+) (pilB) [Acinetobacter sp. YWS30-1]
ATGACAGCATTGCAGGGGTCACCAAGATTTACGGGGTTTATTCGTCGCCTGGTCGATGAAGGGGTATTATCAGCTGAAGA
TATGCGGACTGCTTTAGCCAATGCCAAACAGGAAAAAACAGATATCGTGGCCTATTTGGTCAGTCATCATAATCTTTCCC
CTACTACCATTGCTGAAACGATCTCAGTAGAATTTGGTGAGCCTCTATTTGATATTGGTGCTTATGATCCGGGACAAATC
CTGCGTGAAGATATTGATGAAAAGCTGATTACCAAGCATCGTATTCTGCCAATTTATAAAAACGCCAATATTCTTTATGT
CGCCACGAGCAATCCGACCAATATCGAAGCAACGGATGCCATCCGCTTTGCAACCAAACTGAATATTGAAACTGTTATTG
TTGAACACAATAAACTCGAAAAACTGATTGAGCAAAACTTTACTGAAGAAAGTACTTTTGATTTTGATGATGATTTCGAT
CTGGATGTAAATGTCGATACAGCCGATCCGACTCAAGAAGAAGACGAAAAAGACAAGGGTGAAGAAGCCCCTATTGTTAA
ATATATTAATAAGTTACTGATTGATGCTATTCGTATGGGGGCATCAGATTTACATTTTGAACCTTATGAAAAAATGTATC
GGGTGCGCTATCGTGTCGATGGGGTTTTACGTCAGATTGCAACACCGCCGCTACAACTGGCAAACCGTTTGGCGTCGCGT
TTGAAAGTCATGTCGCAGATGGACATTTCTGAGAAACGCGTGCCTCAAGACGGCCGTATTAAATTAAAGCTCTCAAAAAA
CAAAGCCATCGACTTCCGTGTTAACTCGCTCCCTACCCTGTTTGGCGAAAAACTGGTTCTACGTATTTTAGATCCGTCTA
GTGCCATGCTGGGAATTGATGCTTTAGGTTATGAACCTGAGCAGAAAGAATTATTTATGGAAGCCTTAGATAAACCGCAA
GGGATGTTACTGATTACCGGTCCTACTGGTTCCGGTAAAACAGTATCCCTTTATACGGGCTTAAACATCTTAAATCGTGA
AGATACCAATATTTCTACTGCGGAAGACCCAGTCGAGATTAACCTGCAAGGGATTAATCAGGTGAACGTCAATAATAAAG
TTGGCCTGACGTTTGCAGCTGCTTTAAAATCCTTTCTGCGTCAGGATCCTGATATTGTCATGGTCGGTGAGATCCGTGAT
CTGGAAACAGCTGAAATTGCAATCAAAGCAGCGCAAACAGGTCATATGGTGATGTCAACTCTGCACACCAACAGTGCTCC
GGAAACTTTGACCCGTTTACGTAATATGGGCGTACCTTCTTTCAATATCGCGACTTCAGTCAATCTGGTGATTGCGCAGC
GTCTGGCCCGTCGCTTGTGTTCACAATGTAAAAAACCGGCTGACATTCCAAAGCAAAGCTTACTGGAGATGGGCTTTACC
GAAGCAGATTTACAAAGCCCTGAATTTCAGATTTATGAGCCAGTCGGCTGTAATGAATGTCGTGAAGGTTATAAGGGCCG
GGTCGGTATTTATGAAGTTATGAAGGTAACACCTGAGATTTCAAGAATTATTATGGAAGATGGCAATGCCATTGAAATTG
CTGAAGCTTCCGCACGTGCAGGATTTAACAATTTACGCCGATCAGGGTTAGTGAAGGTTATGCAGGGGGTGACTTCTTTA
CAGGAAGTCAATCGTGTAACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.404

100

0.815
  pilB Acinetobacter baylyi ADP1

76.491

100

0.766
  pilB Legionella pneumophila strain ERS1305867

51.59

99.473

0.513
  pilF Neisseria gonorrhoeae MS11

49.02

98.594

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

49.013

97.891

0.48
  pilB Vibrio campbellii strain DS40M4

48.644

97.188

0.473
  pilB Vibrio cholerae strain A1552

50.677

90.861

0.46
  pilF Thermus thermophilus HB27

38.489

100

0.385
  pilB/pilB1 Synechocystis sp. PCC 6803

34.747

100

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.319

97.012

0.362