Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O4J62_RS01390 Genome accession   NZ_CP114601
Coordinates   309202..310914 (-) Length   570 a.a.
NCBI ID   WP_323712660.1    Uniprot ID   -
Organism   Acinetobacter towneri strain TT1-3_T     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 304202..315914
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O4J62_RS01360 (O4J62_01360) - 304236..304559 (+) 324 WP_004975854.1 pyrimidine/purine nucleoside phosphorylase -
  O4J62_RS01365 (O4J62_01365) rlmB 304748..305497 (+) 750 WP_004975856.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  O4J62_RS01370 (O4J62_01370) - 305592..306470 (+) 879 WP_042068576.1 DMT family transporter -
  O4J62_RS01375 (O4J62_01375) coaE 306476..307075 (-) 600 WP_323712659.1 dephospho-CoA kinase -
  O4J62_RS01380 (O4J62_01380) pilD 307085..307945 (-) 861 WP_004975862.1 prepilin peptidase Machinery gene
  O4J62_RS01385 (O4J62_01385) pilC 307945..309171 (-) 1227 WP_179997302.1 type II secretion system F family protein Machinery gene
  O4J62_RS01390 (O4J62_01390) pilB 309202..310914 (-) 1713 WP_323712660.1 type IV-A pilus assembly ATPase PilB Machinery gene
  O4J62_RS01395 (O4J62_01395) tpiA 311192..311983 (+) 792 WP_019836081.1 triose-phosphate isomerase -
  O4J62_RS01400 (O4J62_01400) secG 311997..312326 (+) 330 WP_004975868.1 preprotein translocase subunit SecG -
  O4J62_RS01425 (O4J62_01425) rimP 313278..313802 (+) 525 WP_004975871.1 ribosome maturation factor RimP -
  O4J62_RS01430 (O4J62_01430) nusA 313838..315322 (+) 1485 WP_131261098.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63541.68 Da        Isoelectric Point: 4.7763

>NTDB_id=766792 O4J62_RS01390 WP_323712660.1 309202..310914(-) (pilB) [Acinetobacter towneri strain TT1-3_T]
MSVQQATPRFTGFIRRLVEDGLVSAENMQTAIKAAQKDNVDIVPYLIQNLNIAPLIIAEKISNEFGEPIFDLSVYDSALI
VREGIDEKLFSKYNLLPIFKRNHLLYVATSNPTNIEAMDAIRFNSKMNIEAIVVEHDKLVKLIEQNFASESAFEFEDDDF
DLNFEDGNSEPEQAAEDEPQGDESPIVKYINKLLIDAIRMDASDLHFEPYEKTYRVRYRVDGVLRQIATPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKNKSIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKELFMEALEKP
QGMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLTFAAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPHCKAPSDIPKQSLLEMGF
TEADLSNPEFQVYQPVGCSECREGYKGRVGVYEVMKVTPEISRIIMEDGNAIQIADASAKSGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=766792 O4J62_RS01390 WP_323712660.1 309202..310914(-) (pilB) [Acinetobacter towneri strain TT1-3_T]
ATGTCAGTACAACAAGCTACACCTAGATTTACAGGTTTTATTCGACGTTTGGTCGAAGATGGGCTCGTTTCTGCTGAAAA
TATGCAAACTGCCATTAAAGCCGCACAAAAAGATAATGTTGATATTGTTCCCTATCTCATTCAAAACCTAAATATCGCGC
CTTTAATCATTGCTGAAAAAATTTCTAATGAGTTTGGCGAACCTATTTTTGATTTAAGCGTGTATGACTCTGCGCTCATC
GTGCGTGAAGGCATTGATGAAAAGCTTTTCAGCAAATATAACCTTTTACCTATTTTTAAGCGCAATCACCTGCTTTACGT
TGCGACCAGTAACCCGACCAATATCGAAGCAATGGATGCCATTCGTTTCAACAGTAAAATGAATATTGAAGCCATTGTGG
TCGAACACGATAAATTGGTAAAACTGATTGAACAAAACTTTGCGAGTGAGTCTGCTTTTGAATTTGAAGATGATGACTTC
GATCTCAACTTTGAAGATGGTAACAGCGAACCAGAACAAGCTGCAGAAGATGAACCGCAGGGTGATGAATCCCCAATTGT
TAAATACATCAACAAATTACTGATTGATGCAATTCGCATGGATGCTTCGGACTTACACTTTGAACCGTATGAAAAAACCT
ATCGAGTGCGTTACCGTGTCGATGGTGTATTACGTCAAATTGCCACCCCACCCTTACAGTTAGCTAACCGCTTGGCATCA
CGTTTAAAAGTGATGTCCCAAATGGACATTTCTGAAAAGCGTGTCCCGCAAGATGGTCGTATTAAATTAAAACTATCTAA
AAACAAGTCCATCGACTTCCGTGTGAACTCGTTGCCAACATTGTTCGGTGAAAAAATCGTACTGCGTATTCTTGACCCAT
CTAGCGCGATGCTTGGGATTGACGCTTTGGGCTATGAACCAGAACAAAAAGAATTGTTCATGGAAGCCTTAGAAAAACCG
CAAGGCATGTTACTGATTACAGGCCCAACGGGTTCGGGTAAAACGGTATCGCTATATACAGGCTTAAATATCTTAAACAG
AGAAGATACCAACATTTCCACCGCCGAAGACCCTGTGGAAATTAACTTAGAAGGCATCAATCAGGTTAACGTCAACAATA
AAGTAGGCTTAACCTTCGCTGCCGCCCTGAAATCTTTCTTACGTCAAGACCCTGATATTGTCATGGTGGGTGAGATTCGT
GATCTTGAAACTGCAGAAATTGCAATTAAAGCTGCCCAAACAGGTCACATGGTTTTATCTACCCTACACACCAACAGTGC
GCCAGAAACTTTAACGCGTTTACGCAACATGGGCGTTCCTTCCTTTAACATCGCAACTTCGGTAAACCTCGTCATTGCAC
AGCGTTTGGCACGACGCCTATGCCCGCATTGCAAAGCTCCTTCCGATATTCCAAAACAAAGCTTATTGGAAATGGGCTTT
ACTGAAGCCGATTTGAGCAATCCAGAATTTCAGGTCTATCAACCTGTAGGCTGTTCTGAATGCCGTGAAGGTTATAAAGG
ACGTGTTGGTGTTTATGAAGTGATGAAAGTCACACCAGAAATTTCTAGAATTATTATGGAGGATGGTAATGCCATCCAAA
TTGCTGATGCGTCAGCAAAATCGGGGTTTAACAATCTGCGTCGTTCGGGGTTACTTAAGGTCATGCAGGGGGTGACTTCA
TTGCAAGAAGTCAATCGTGTGACCAGTGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

77.895

100

0.779
  pilB Acinetobacter baylyi ADP1

76.14

100

0.761
  pilB Legionella pneumophila strain ERS1305867

50.172

100

0.511
  pilF Neisseria gonorrhoeae MS11

49.644

98.596

0.489
  pilB Vibrio cholerae strain A1552

48.41

99.298

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

48.214

98.246

0.474
  pilB Vibrio campbellii strain DS40M4

50.482

91.053

0.46
  pilF Thermus thermophilus HB27

37.608

100

0.381
  pilB/pilB1 Synechocystis sp. PCC 6803

34.733

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.722

98.596

0.372