Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   N7E60_RS01235 Genome accession   NZ_CP114584
Coordinates   257444..259189 (+) Length   581 a.a.
NCBI ID   WP_108743819.1    Uniprot ID   -
Organism   Salinivibrio proteolyticus strain TGB10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 252444..264189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7E60_RS01215 (N7E60_01215) pilM 254827..255741 (+) 915 WP_269597924.1 pilus assembly protein PilM -
  N7E60_RS01220 (N7E60_01220) - 255732..256310 (+) 579 WP_269597925.1 PilN domain-containing protein -
  N7E60_RS01225 (N7E60_01225) - 256307..256918 (+) 612 WP_269597926.1 type 4a pilus biogenesis protein PilO -
  N7E60_RS01230 (N7E60_01230) - 256915..257427 (+) 513 WP_077455115.1 pilus assembly protein PilP -
  N7E60_RS01235 (N7E60_01235) pilQ 257444..259189 (+) 1746 WP_108743819.1 type IV pilus secretin PilQ Machinery gene
  N7E60_RS01240 (N7E60_01240) aroK 259335..259853 (+) 519 WP_069589875.1 shikimate kinase AroK -
  N7E60_RS01245 (N7E60_01245) aroB 259875..260954 (+) 1080 WP_269597927.1 3-dehydroquinate synthase -
  N7E60_RS01250 (N7E60_01250) - 260966..262531 (+) 1566 WP_269597928.1 AAA family ATPase -
  N7E60_RS01255 (N7E60_01255) dam 262604..263425 (+) 822 WP_069589882.1 adenine-specific DNA-methyltransferase -
  N7E60_RS01260 (N7E60_01260) - 263439..263627 (-) 189 WP_069589884.1 DUF2970 domain-containing protein -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63275.84 Da        Isoelectric Point: 6.5385

>NTDB_id=766585 N7E60_RS01235 WP_108743819.1 257444..259189(+) (pilQ) [Salinivibrio proteolyticus strain TGB10]
MIRPYLSRTFSQSVRPVWLILLVLMFSLGSVQVAAKSMLTDIAVKRLDNGDAQVSLTFSDAAVTADFRQPIPQQVTLRLP
NVQLKQALLAVLDTRSRQTPVTEIETFQAPESARIVLRAEQAVDFDYQQQGKVLTIRVSAAKKAPSATALAQKGAAISIN
FQDIPVRTVLQLIAEQNDFNLVTSDSVQGNITMRIDGVPWQDVLDVILRVKGLDKRQRGQVMLVAPSAELMAQEKQALEM
RQQADALLPLTSEVLTVNYAKASDIAALIKGQGEGVSLLSPRGAVSLDERTNALIIKDNQKNLARIRTLVRHLDVPVKQV
EIEARIVTVEEGSVDELGVRWGILNRNGSTSIGSSIESNLMWDRNMYNSDDEALTGSGNDSLPVDNFLNVNLAASNPAAS
SLALQVAKLGSDLLLDLELSALESENKAEIVSSPRLITTNKKAAYIEQGTELPYLEASSSGAVAVSFKKAVLSLTVTPQI
TPENELVLDLKVTQDMPEKSVVAGKGEAMSISTKRINTQVLARNGETVVLGGIFQHTMINKETKVPLLGDIPLFGNLFKH
RLKDHAKRELLIFVTPKIVTG

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=766585 N7E60_RS01235 WP_108743819.1 257444..259189(+) (pilQ) [Salinivibrio proteolyticus strain TGB10]
ATGATACGCCCTTACCTTTCCCGCACGTTCTCGCAAAGCGTTCGCCCAGTCTGGCTCATACTGCTGGTACTGATGTTCAG
CCTGGGTTCAGTGCAGGTTGCCGCAAAGAGTATGCTGACTGATATCGCAGTGAAGCGTCTAGATAATGGTGATGCTCAGG
TCTCGCTGACGTTTTCTGATGCCGCGGTAACGGCTGACTTTCGTCAACCCATCCCTCAGCAAGTTACGCTACGCTTGCCG
AATGTACAGCTAAAACAGGCATTACTTGCGGTTCTCGATACTCGCAGCCGTCAGACCCCAGTGACTGAGATTGAGACCTT
TCAGGCCCCTGAGAGTGCTCGCATTGTACTGCGTGCTGAGCAGGCCGTGGATTTTGATTATCAGCAGCAAGGAAAGGTGT
TGACCATCCGCGTCTCTGCGGCAAAAAAAGCGCCTTCTGCGACGGCCTTAGCGCAAAAAGGGGCGGCGATCTCGATTAAC
TTCCAAGACATTCCGGTCCGTACCGTGTTGCAGCTGATTGCTGAACAGAATGATTTTAACCTTGTGACTTCGGACTCGGT
ACAAGGCAACATCACCATGCGCATTGATGGTGTGCCTTGGCAAGATGTATTGGATGTGATTTTGCGTGTGAAGGGACTGG
ATAAGCGCCAACGGGGACAAGTGATGCTCGTTGCTCCGAGCGCTGAGCTGATGGCACAAGAAAAGCAAGCACTCGAAATG
CGTCAGCAGGCCGATGCCTTACTGCCTCTGACCTCAGAAGTACTCACGGTGAACTATGCCAAAGCCAGTGATATCGCCGC
CCTGATCAAAGGCCAAGGCGAAGGGGTCAGCTTACTGTCTCCTCGCGGGGCGGTGTCGCTGGATGAGCGCACCAATGCGT
TGATCATTAAAGATAATCAAAAGAATTTAGCCCGTATTCGCACCTTGGTCCGCCACTTGGATGTGCCGGTGAAGCAAGTT
GAAATTGAAGCGCGGATTGTCACGGTTGAAGAGGGGTCTGTCGATGAGCTGGGAGTGCGCTGGGGGATTTTAAATCGCAA
CGGTTCAACCAGTATCGGCTCTTCGATAGAAAGCAACCTGATGTGGGACCGTAACATGTACAATTCCGATGACGAGGCAT
TAACCGGATCGGGCAATGATTCTCTGCCGGTGGACAATTTTTTGAATGTGAACTTGGCCGCCTCCAACCCTGCCGCGTCA
AGTCTGGCACTACAAGTGGCTAAATTGGGCAGTGATTTATTGCTGGATCTCGAGTTATCGGCGCTGGAAAGTGAAAACAA
AGCAGAAATTGTATCGAGCCCGCGCTTAATCACCACCAATAAAAAAGCGGCATATATCGAGCAGGGGACAGAGTTACCTT
ATCTCGAAGCCTCTTCCAGCGGTGCTGTGGCGGTGTCGTTTAAAAAAGCAGTACTTAGCCTTACCGTGACGCCCCAAATT
ACCCCAGAGAATGAGTTAGTGCTGGATTTGAAAGTCACCCAAGATATGCCAGAGAAGTCGGTGGTGGCAGGTAAAGGGGA
GGCCATGTCGATCAGTACCAAGCGCATTAATACGCAGGTATTGGCGCGGAATGGTGAAACGGTGGTGCTGGGAGGGATCT
TCCAACACACCATGATTAACAAAGAAACCAAGGTGCCATTACTCGGGGATATTCCGCTGTTTGGAAATCTGTTCAAACAC
CGTCTGAAAGATCATGCAAAACGCGAGCTTCTGATCTTTGTGACCCCTAAAATCGTCACAGGGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(253-319)

Bacterial type II and III secretion system protein (Secretin)

(421-579)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

55.451

91.566

0.508
  pilQ Vibrio cholerae strain A1552

57.198

88.468

0.506
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

57.198

88.468

0.506
  comQ Acinetobacter baylyi ADP1

38.648

99.312

0.384
  pilQ Acinetobacter baumannii strain A118

37.119

100

0.377
  pilQ Acinetobacter baumannii D1279779

37.119

100

0.377
  pilQ Legionella pneumophila strain Lp02

39.138

95.869

0.375
  pilQ Legionella pneumophila strain ERS1305867

39.138

95.869

0.375
  pilQ Pseudomonas aeruginosa PAK

37.943

97.074

0.368