Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CF557_RS07180 Genome accession   NZ_CP114281
Coordinates   1527022..1528431 (-) Length   469 a.a.
NCBI ID   WP_094871618.1    Uniprot ID   -
Organism   Gallibacterium anatis strain ESV200     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1522022..1533431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CF557_RS07145 (CF557_07145) - 1522306..1522965 (+) 660 WP_013746615.1 formate dehydrogenase subunit gamma -
  CF557_RS07150 (CF557_07150) fdhE 1522967..1523890 (+) 924 WP_094871610.1 formate dehydrogenase accessory protein FdhE -
  CF557_RS07155 (CF557_07155) - 1523956..1524228 (-) 273 WP_039148365.1 GNAT family N-acetyltransferase -
  CF557_RS07160 (CF557_07160) yacG 1524314..1524532 (-) 219 WP_018345915.1 DNA gyrase inhibitor YacG -
  CF557_RS07165 (CF557_07165) coaE 1524522..1525154 (-) 633 WP_094871613.1 dephospho-CoA kinase -
  CF557_RS07170 (CF557_07170) - 1525169..1525801 (-) 633 WP_256963499.1 prepilin peptidase -
  CF557_RS07175 (CF557_07175) - 1525798..1527006 (-) 1209 WP_094871616.1 type II secretion system F family protein -
  CF557_RS07180 (CF557_07180) pilB 1527022..1528431 (-) 1410 WP_094871618.1 GspE/PulE family protein Machinery gene
  CF557_RS07185 (CF557_07185) pilA 1528448..1528909 (-) 462 WP_094871620.1 prepilin peptidase-dependent pilin Machinery gene
  CF557_RS07190 (CF557_07190) ampD 1529014..1529568 (+) 555 WP_039086466.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CF557_RS07195 (CF557_07195) - 1529655..1529990 (+) 336 WP_013746603.1 hypothetical protein -
  CF557_RS07200 (CF557_07200) - 1530099..1531493 (+) 1395 WP_094871623.1 chloride channel protein -
  CF557_RS07205 (CF557_07205) dusA 1531474..1532454 (+) 981 WP_094871625.1 tRNA dihydrouridine(20/20a) synthase DusA -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 52788.22 Da        Isoelectric Point: 5.4692

>NTDB_id=765354 CF557_RS07180 WP_094871618.1 1527022..1528431(-) (pilB) [Gallibacterium anatis strain ESV200]
MGYCVKALDSEQLFEISELQWQKNQQQYELLLRYLAVPLQEQAQQLWLAVESFDNLAACETFAFLSGKQIEPVAIGQTAL
KQLLQQLQPGTATNQVQESQLHSYQQNDIVAKSDEIAEVDAQEPIIQLFNQLLESGLQYSVSDIHLEPLRQEFQIRFRID
GVLQRHQVLTLSIGQRLNSRLKLLAKLDINELRLPQDGRFRFKTAFGETLDIRLSSLPTQYGEKIVLRLQPNTPVFDDFT
ELGMTNEQAVLFRQALSQPQGLILVTGPTGSGKSLTLYSGLHFLNQESRHILTAEDPIELQLNGIIQTQANAAIGLTFAT
LLRSFLRQDPDVIMVGEIRDQETAEIALRAAQTGHLVLSTLHTNTAIGAISRLQQLGIAQHELENSLLLVVAQRLVRKRC
TLCQGDKSQQCDCVGGYRGRTGVYQLLHYQQQQIQMDYPTLLAAAQLKLQQRITTTAEVERVFGKTENS

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=765354 CF557_RS07180 WP_094871618.1 1527022..1528431(-) (pilB) [Gallibacterium anatis strain ESV200]
ATGGGCTATTGTGTAAAGGCATTGGATTCGGAGCAGCTGTTTGAAATTTCAGAATTGCAATGGCAGAAGAATCAACAGCA
ATATGAACTGTTATTGCGTTATTTAGCTGTACCGTTGCAGGAACAAGCGCAACAGTTATGGTTAGCGGTTGAGTCTTTCG
ATAATTTAGCAGCCTGCGAAACCTTCGCTTTTCTTTCCGGCAAACAGATTGAGCCTGTTGCGATCGGACAAACTGCACTG
AAACAGTTATTACAGCAGCTGCAACCCGGAACGGCGACAAATCAAGTGCAAGAATCACAACTGCATAGTTATCAGCAAAA
TGATATTGTCGCAAAATCGGATGAAATTGCGGAAGTTGATGCACAAGAGCCGATTATTCAACTATTCAACCAATTATTGG
AATCAGGTTTGCAATATTCCGTTTCTGATATTCATCTTGAGCCGTTACGTCAGGAGTTCCAGATCCGCTTTAGAATTGAC
GGCGTATTACAACGGCATCAGGTACTGACGTTGTCAATAGGTCAACGCTTAAACTCACGCCTAAAATTGTTGGCAAAACT
GGATATTAACGAATTACGCTTGCCGCAGGATGGTCGTTTTCGTTTTAAAACCGCATTCGGTGAAACATTAGATATTCGGT
TATCTTCTCTACCGACACAATATGGTGAAAAAATCGTATTACGATTGCAACCTAACACACCGGTGTTTGATGACTTTACC
GAACTGGGAATGACAAACGAGCAGGCTGTGCTGTTCCGGCAAGCGTTATCTCAACCGCAAGGATTAATCTTGGTTACCGG
ACCGACCGGAAGTGGTAAAAGTTTGACATTATATAGCGGTTTACATTTTTTAAATCAGGAAAGTCGCCACATTCTTACAG
CGGAAGATCCGATAGAACTGCAATTAAACGGCATTATTCAGACTCAAGCAAATGCCGCAATCGGTTTAACTTTTGCAACA
TTATTGCGGAGTTTTTTACGCCAAGATCCGGATGTCATTATGGTTGGGGAAATTCGAGATCAGGAAACGGCAGAGATAGC
ATTACGCGCGGCACAGACGGGGCATTTAGTGTTATCGACACTGCATACCAATACGGCAATCGGTGCAATTTCCCGATTAC
AACAGTTGGGAATTGCACAACACGAATTGGAAAATTCATTATTGCTGGTGGTGGCACAACGTTTAGTCCGCAAACGTTGC
ACCTTATGCCAAGGTGATAAATCTCAGCAATGCGATTGTGTTGGCGGTTATCGCGGACGAACCGGCGTTTATCAACTGCT
ACATTATCAGCAACAACAAATTCAAATGGATTATCCGACATTATTGGCGGCAGCACAGCTTAAATTACAGCAACGGATCA
CGACAACAGCAGAGGTTGAACGCGTCTTCGGCAAGACGGAGAACAGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-91)

Type II/IV secretion system protein (T2SSE)

(131-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

59.43

97.228

0.578
  pilB Haemophilus influenzae 86-028NP

59.43

97.228

0.578
  pilB Glaesserella parasuis strain SC1401

53.632

99.787

0.535
  pilB Vibrio parahaemolyticus RIMD 2210633

38.878

100

0.414
  pilB Vibrio cholerae strain A1552

40.041

100

0.412
  pilB/pilB1 Synechocystis sp. PCC 6803

36.923

100

0.409
  pilB Vibrio campbellii strain DS40M4

39.627

100

0.407
  pilB Legionella pneumophila strain ERS1305867

36.157

100

0.373
  pilB Acinetobacter baylyi ADP1

39.503

94.456

0.373
  pilB Acinetobacter baumannii D1279779

39.13

93.177

0.365
  pilF Neisseria gonorrhoeae MS11

35.356

100

0.36