Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CF557_RS07185 Genome accession   NZ_CP114281
Coordinates   1528448..1528909 (-) Length   153 a.a.
NCBI ID   WP_094871620.1    Uniprot ID   -
Organism   Gallibacterium anatis strain ESV200     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1523448..1533909
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CF557_RS07155 (CF557_07155) - 1523956..1524228 (-) 273 WP_039148365.1 GNAT family N-acetyltransferase -
  CF557_RS07160 (CF557_07160) yacG 1524314..1524532 (-) 219 WP_018345915.1 DNA gyrase inhibitor YacG -
  CF557_RS07165 (CF557_07165) coaE 1524522..1525154 (-) 633 WP_094871613.1 dephospho-CoA kinase -
  CF557_RS07170 (CF557_07170) - 1525169..1525801 (-) 633 WP_256963499.1 prepilin peptidase -
  CF557_RS07175 (CF557_07175) - 1525798..1527006 (-) 1209 WP_094871616.1 type II secretion system F family protein -
  CF557_RS07180 (CF557_07180) pilB 1527022..1528431 (-) 1410 WP_094871618.1 GspE/PulE family protein Machinery gene
  CF557_RS07185 (CF557_07185) pilA 1528448..1528909 (-) 462 WP_094871620.1 prepilin peptidase-dependent pilin Machinery gene
  CF557_RS07190 (CF557_07190) ampD 1529014..1529568 (+) 555 WP_039086466.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CF557_RS07195 (CF557_07195) - 1529655..1529990 (+) 336 WP_013746603.1 hypothetical protein -
  CF557_RS07200 (CF557_07200) - 1530099..1531493 (+) 1395 WP_094871623.1 chloride channel protein -
  CF557_RS07205 (CF557_07205) dusA 1531474..1532454 (+) 981 WP_094871625.1 tRNA dihydrouridine(20/20a) synthase DusA -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 16359.71 Da        Isoelectric Point: 8.0868

>NTDB_id=765355 CF557_RS07185 WP_094871620.1 1528448..1528909(-) (pilA) [Gallibacterium anatis strain ESV200]
MKKREQLKSNYAGFTLIELMIVIAIIAVLATIAIPSYQNYTKKAAISELIQASSPYKAEVELCIYNTKSKASCNAGQNGI
QAKPSNMDKFKYLKEVNVTAGVISVSGKPSLDGISYTMTPSDGNNGELIDWSVTCSGDNGLFPAGFCSNTSAN

Nucleotide


Download         Length: 462 bp        

>NTDB_id=765355 CF557_RS07185 WP_094871620.1 1528448..1528909(-) (pilA) [Gallibacterium anatis strain ESV200]
ATGAAAAAACGTGAACAACTTAAGTCGAATTATGCCGGATTTACTTTAATCGAATTGATGATCGTCATTGCGATTATTGC
TGTGTTGGCAACCATTGCCATTCCCTCTTATCAAAATTACACCAAAAAGGCGGCAATATCAGAATTGATTCAGGCCTCCT
CACCCTATAAGGCGGAAGTTGAACTCTGTATTTATAACACTAAGAGTAAAGCAAGCTGTAACGCCGGTCAAAACGGCATT
CAGGCGAAGCCGTCTAATATGGATAAATTTAAATATCTTAAAGAGGTTAATGTAACAGCTGGCGTTATTTCTGTTTCTGG
TAAGCCAAGTCTAGATGGGATTAGTTATACAATGACCCCAAGTGACGGTAATAACGGTGAATTAATTGATTGGAGTGTAA
CTTGTAGCGGTGATAATGGGCTGTTTCCGGCAGGATTTTGTAGCAATACTTCTGCAAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(9-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

54.667

98.039

0.536
  pilA Haemophilus influenzae Rd KW20

52

98.039

0.51
  pilA Haemophilus influenzae 86-028NP

55.882

88.889

0.497